Name: Anti-MUC2 antibody [EPR23479-47]
SKU: ab272692
Rating: 5 (7 reviews)

Anti-MUC2 antibody [EPR23479-47] is a rabbit recombinant monoclonal antibody that is used to detect MUC2 in IHC-Fr, IHC-P, Western blot, mIHC. Suitable for Human, Mouse, Rat samples.

- Cited in over 35 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency

Images

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (AB272692), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	mIHC	IP	Flow Cyt	WB	ICC/IF	IHC-Fr	IHC-P
Human	Tested	Not recommended	Not recommended	Tested	Not recommended	Expected	Tested
Mouse	Tested	Not recommended	Not recommended	Tested	Not recommended	Tested	Tested
Rat	Tested	Not recommended	Not recommended	Tested	Not recommended	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/2000 - 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Species Rat	Dilution info 1/500	Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information MUC2

Function

Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed:17058067, PubMed:19432394, PubMed:33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed:19432394, PubMed:33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed:33031746, PubMed:36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed:36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites: Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed:36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed:36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed:33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed:33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon: a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).

Alternative names

SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR23479-47
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-MUC2 antibody [EPR23479-47] (ab272692) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-Fr, IHC-P, WB, mIHC in human, mouse, rat samples.
Anti-MUC2 antibody [EPR23479-47] (ab272692) specifically detects MUC2 (UniProt ID: Q02817; Molecular weight: 549kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-MUC2 antibody [EPR23479-47] (ab272692) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-MUC2 antibody [EPR23479-47] (ab272692) has been cited over 40 times in peer reviewed journals and is trusted by the scientific community.
Anti-MUC2 antibody [EPR23479-47] (ab272692) has 4 independent reviews from customers.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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16 product images

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). Positive staining on rat colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling MUC2 with ab272692 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). Positive staining on mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor^® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MUC2 is highly glycosylated.
The C-terminal fragment alpha-MUC2C2 (140-170 kDa) is observed. The molecular weight is consistent with what have been described in literature (PMID: 12582180; 16754877). Exposure time: 3 seconds.
All lanes: Western blot - Anti-MUC2 antibody [EPR23479-47] (ab272692) at 1/1000 dilution
Lane 1: Human colon cancer tissue lysate at 20 µg
Lane 2: Mouse small intestine tissue lysate at 20 µg
Lane 3: Rat small intestine tissue lysate at 20 µg
Lane 4: Rat colon tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 540 kDa
Observed band size: 140-170 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on rat colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on mouse colon is observed. The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on human bladder cancer (PMID: 25197366). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MUC2 with ab272692 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Postive staining on human colon (PMID: 28693267). The section was incubated with ab272692 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Fluorescence multiplex immunohistochemical analysis of the human jejunum (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Muc2 (ab272692, magenta; Opal™690), anti-Eph receptor B2 (Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, green; Opal™520) and anti-ErbB3 / HER3 (Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, red; Opal™570) on human jejunum. Panel B: anti-Muc2 stained on goblet cells. Panel C: anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D: anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of ab272692 at 1/2000 dilution (0.252 μg/ml), Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/1000 dilution (0.527 μg/ml), and Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections).

Panel A: merged staining of anti-Muc2 (ab272692, magenta; Opal™690), anti-Eph receptor B2 (Anti-Eph receptor B2 antibody [EPR22427-268] ab252935, green; Opal™520) and anti-ErbB3 / HER3 (Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220, red; Opal™570) on human colon. Panel B: anti-Muc2 stained on goblet cells. Panel C: anti-Eph receptor B2 stained on intestinal stem and progenitor cells. Panel D: anti-ErbB3 / HER3 stained on epithelial cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

The section was incubated in three rounds of staining: in the order of ab272692 at 1/2000 dilution (0.252 μg/ml), Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1/1000 dilution (0.527 μg/ml), and Anti-ErbB3 / HER3 antibody [SP71] - BSA and Azide free ab236220 at 1/3000 dilution (0.75 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
MUC2 Multiplex immunohistochemistry staining of Rat small intestine tissue using rabbit Anti-MUC2 antibody
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat small intestine.
Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat small intestine.
Panel C: anti-MUC2 staining goblet cells in rat small intestine.
Panel D: anti-Eph receptor B2 staining membrane of stem cells in rat small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Fluorescence multiplex immunohistochemical analysis of the human colon (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Villin (Anti-Villin antibody [SP145] - BSA and Azide free ab245749, gray; Opal™690), anti-liver FABP (Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401, green; Opal™520) and anti-MUC2 (ab272692, red; Opal™570) on human colon. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Villin stained on apical border. Panel D: anti-MUC2 stained on goblet cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Villin antibody [SP145] - BSA and Azide free ab245749 (1/1000 dilution), Anti-liver FABP antibody [EPR20464] - BSA and Azide free ab240401 (1/8000 dilution), and ab272692 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
MUC2 Multiplex immunohistochemistry staining of Rat colon tissue using rabbit Anti-MUC2 antibody
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections rat colon tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on rat colon.
Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in rat colon.
Panel C: anti-MUC2 staining goblet cells in rat colon.
Panel D: anti-Eph receptor B2 staining membrane of stem cells in rat colon.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
MUC2 Multiplex immunohistochemistry staining of Mouse small intestine tissue using rabbit Anti-MUC2 antibody
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse small intestine tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse small intestine.
Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse small intestine.
Panel C: anti-MUC2 staining goblet cells in mouse small intestine.
Panel D: anti-Eph receptor B2 staining membrane of stem cells in mouse small intestine.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
MUC2 Multiplex immunohistochemistry staining of Mouse colon tissue using rabbit Anti-MUC2 antibody
Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections mouse colon tissue labelling Sodium/Hydrogen Exchanger 3 with Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365 at 1:5000 dilution (B), MUC2 with ab272692 at 1:2000 dilution (C) Eph receptor B2 with Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 at 1:500 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and
DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-Sodium/Hydrogen Exchanger 3 (green; Opal™520), anti-MUC2 (magenta; Opal™690) and anti-Eph receptor B2 (gray; Opal™570) on mouse colon.
Panel B: anti-Sodium/Hydrogen Exchanger 3 staining apical and luminal facing edges of surface cells in mouse colon.
Panel C: anti-MUC2 staining goblet cells in mouse colon.
Panel D: anti-Eph receptor B2 staining membrane of stem cells in mouse colon.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody [EPR26951-3] ab307365, ab272692 and Anti-Eph receptor B2 antibody [EPR22427-268] ab252935 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human small intestine tissue staining MUC17 with Anti-MUC17 antibody [EPR28482-85] ab316971 at 1/500 dilution, ab272692 anti-MUC2 used at 1/2000 dilution and Anti-REG1B antibody [EPR28864-4] ab323315 anti-REG1B used at a 1/5000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-MUC17 (green; Opal^™570), anti-MUC2 (magenta; Opal^™690) and anti-REG1B (grey; Opal^™520) on human small intestine.

Panel B: anti-MUC17 staining epithelium in human small intestine.

Panel C: anti-MUC2 staining goblet cells in human small intestine.

Panel D: anti-REG1B staining Paneth cells in human small intestine.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-MUC17 antibody [EPR28482-85] ab316971, ab272692 and Anti-REG1B antibody [EPR28864-4] ab323315 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-MUC2 antibody [EPR23479-47] (ab272692)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse colon tissue staining GSDMC2 + GSDMC3 with Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] ab229896 at a 1:2000 (0.25 ug/ml) dilution; SARM1 with Anti-SARM1 antibody [EPR24834-80] ab309195 at 1:500 (0.964 ug/ml) dilution and MUC2 with ab272692 at 1:2000 (0.252 ug/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-SARM1 (green; Opal^™520), anti-MUC2 (gray; Opal^™570) and anti-GSDMC2/3 (magenta; Opal^™690)on mouse colon.

Panel B: anti-SARM1 staining myenteric nerve plexus in mouse colon.

Panel C: anti-MUC2 staining goblet cells in mouse colon.

Panel D: anti-GSDMC2/3 staining epithelium in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-SARM1 antibody [EPR24834-80] ab309195, ab272692 and Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] ab229896 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins