Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture)

• IP

Lab

Immunoprecipitation - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

MUC2 was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab281261 at 1/50 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281261 at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261) at 1/1000 dilution

Lane 1:

Human colon tissue lysate (Input) at 10 µg

Lane 2:

Human colon tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab281261 in Human colon tissue lysate (-)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 140-170 kDa

false

Exposure time: 41s

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh frozen) tissue labeling MUC2 with ab281261 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green).

Negative control : confocal image showing no staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab281261 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat colon (fresh frozen) tissue labeling MUC2 with ab281261 at 1/1000 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green).

Confocal image showing positive staining on rat colon. The nuclear counterstain was DAPI (Blue). The section was incubated with ab281261 for 60 mins at room temperature. The section was then mounted using Fluoromount^®.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

• WB

Lab

Western blot - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (ab281261) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 10 µg

Lane 2:

Human small intestine tissue lysate at 10 µg

Lane 3:

Rat colon tissue lysate at 10 µg

Lane 4:

Rat small intestine tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 140-170 kDa

false

Exposure time: 26s

• sELISA

Supplier Data

Sandwich ELISA - Anti-MUC2 antibody [EPR23479-88] - BSA and Azide free (Capture) (AB281261)

Example of Human MUC2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.