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AB134119

Anti-MUC2 antibody [EPR6145]

5

(2 Reviews)

|

(30 Publications)

Rabbit Recombinant Monoclonal MUC2 antibody. Suitable for IHC-P, WB, I-ELISA and reacts with Human, Synthetic peptide - Human samples. Cited in 30 publications.

View Alternative Names

SMUC, MUC2, Mucin-2, MUC-2, Intestinal mucin-2

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using unpurified ab134119 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab134119 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with unpurified ab134119 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab134119 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using unpurified ab134119 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [EPR6145] (AB134119)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using unpurified ab134119 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MUC2 antibody [EPR6145] (AB134119)
  • WB

Lab

Western blot - Anti-MUC2 antibody [EPR6145] (AB134119)

The 110kda band is the C-terminal cleaved fragment, formed by an autocatalytic mechanism triggered by low pH. (PMID : 12582180).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MUC2 antibody [EPR6145] (ab134119) at 1/2000 dilution

Lane 1:

Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Human small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 540 kDa

Observed band size: 110 kDa

false

Exposure time: 3min

Western blot - Anti-MUC2 antibody [EPR6145] (AB134119)
  • WB

Lab

Western blot - Anti-MUC2 antibody [EPR6145] (AB134119)

MUC2 could be cleaved into three fragments with the molecular weight 250, 130 and 110 KD as is described in PMID : 12582180.

All lanes:

Western blot - Anti-MUC2 antibody [EPR6145] (ab134119) at 1/10000 dilution

All lanes:

Human colon lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 540 kDa

Observed band size: 130 kDa

false

Indirect ELISA - Anti-MUC2 antibody [EPR6145] (AB134119)
  • I-ELISA

Unknown

Indirect ELISA - Anti-MUC2 antibody [EPR6145] (AB134119)

Indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/mL) and MUC2 antigen at 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.

OI-RD Scanning - Anti-MUC2 antibody [EPR6145] (AB134119)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MUC2 antibody [EPR6145] (AB134119)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6145

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/15000", "IHCP-species-notes": "<p>Or perform heat mediated antigen retrieval using <a href='/en-us/products/buffers/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab93684'>ab93684</a> (Tris/EDTA buffer, pH 9.0).</p><p><strong>For unpurified use at 1/250-1/500.</strong></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1 µg/mL", "IELISA-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Coats the epithelia of the intestines and other mucus membrane-containing organs to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces (PubMed : 17058067, PubMed : 19432394, PubMed : 33031746). Major constituent of the colon mucus, which is mainly formed by large polymeric networks of MUC2 secreted by goblet cells that cover the exposed surfaces of intestine (PubMed : 19432394, PubMed : 33031746). MUC2 networks form hydrogels that guard the underlying epithelium from pathogens and other hazardous matter entering from the outside world, while permitting nutrient absorption and gas exchange (PubMed : 33031746, PubMed : 36206754). Acts as a divalent copper chaperone that protects intestinal cells from copper toxicity and facilitates nutritional copper unptake into cells (PubMed : 36206754). Binds both Cu(2+) and its reduced form, Cu(1+), at two juxtaposed binding sites : Cu(2+), once reduced to Cu(1+) by vitamin C (ascorbate) or other dietary antioxidants, transits to the other binding site (PubMed : 36206754). MUC2-bound Cu(1+) is protected from oxidation in aerobic environments, and can be released for nutritional delivery to cells (PubMed : 36206754). Mucin gels store antimicrobial molecules that participate in innate immunity (PubMed : 33031746). Mucin glycoproteins also house and feed the microbiome, lubricate tissue surfaces, and may facilitate the removal of contaminants and waste products from the body (PubMed : 33031746). Goblet cells synthesize two forms of MUC2 mucin that differ in branched chain O-glycosylation and the site of production in the colon : a (1) 'thick' mucus that wraps the microbiota to form fecal pellets is produced in the proximal, ascending colon (By similarity). 'Thick' mucus transits along the descending colon and is lubricated by a (2) 'thin' MUC2 mucus produced in the distal colon which adheres to the 'thick' mucus (By similarity).
See full target information MUC2
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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

ESMO gastrointestinal oncology 7: PubMed41069644

2025

Development and characterization of orthotopic patient-derived xenograft models of peritoneal metastatic mucinous appendiceal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ichiaki Ito,Vinay K Pattalachinti,Abdelrahman Mg Yousef,Saikat Chowdhury,Mohammad M Fanaeian,Emaan Haque,Betul Beyza Gunes,Mahmoud Yousef,Emma R Salle,Mohammad A Zeineddine,Shuangxi Ji,Ruonan Li,Wenyi Wang,Beth A Helmink,Melissa W Taggart,Michael G White,Keith F Fournier,Natalie W Fowlkes,John Paul Shen

Scientific reports 15:19813 PubMed40473694

2025

Automated tissue dissociation for the establishment of human intestinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Woo Jin Yang,Eva Blahusova,Komal Nayak,Róisín M Owens,Matthias Zilbauer

BMC biology 22:297 PubMed39716180

2024

A mucus layer derived from porcine intestinal organoid air-liquid interface monolayer attenuates swine enteric coronavirus infection by antiviral activity of Muc2.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Yang,Yang Li,Yifei Cai,Yuanyuan Liu,Yunhang Zhang,Yuguang Fu,Chen Tan,Luc Willems,Guangliang Liu

International journal of biological sciences 20:4476-4495 PubMed39247815

2024

Chronic Stress-induced Serotonin Impairs Intestinal Epithelial Cell Mitochondrial Biogenesis via the AMPK-PGC-1α Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Yang,Yan Sun,Pei Wen,Yaoxing Chen,Jing Cao,Xuelin Sun,Yulan Dong

Journal of virology 98:e0046124 PubMed38780247

2024

Transmissible gastroenteritis virus induces inflammatory responses via RIG-I/NF-κB/HIF-1α/glycolysis axis in intestinal organoids and .

Applications

Unspecified application

Species

Unspecified reactive species

Yunhang Zhang,Ning Yang,Yang Li,Chen Tan,Yifei Cai,Xue Rui,Yuanyuan Liu,Yuguang Fu,Guangliang Liu

Cell death and differentiation 31:618-634 PubMed38424148

2024

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Xu,Zhebin Hua,Yaosheng Wang,Wenbo Tang,Weijun Ou,Fangyuan Liu,Yiqing Yang,Wenjun Ding,Zhongchuan Wang,Long Cui,Wensong Ge,Yubei Gu,Xiaolei Wang,YingWei Chen,Chen-Ying Liu,Peng Du

Microbiome 12:38 PubMed38395946

2024

Multi-omics reveal mechanisms of high enteral starch diet mediated colonic dysbiosis via microbiome-host interactions in young ruminant.

Applications

Unspecified application

Species

Unspecified reactive species

Chunjia Jin,Shengru Wu,Ziqi Liang,Jun Zhang,Xinjian Lei,Hanxun Bai,Gaofeng Liang,Xiaodong Su,Xiaodong Chen,Peiyue Wang,Yue Wang,Leluo Guan,Junhu Yao

Nutrients 15: PubMed37375559

2023

OLL2712 Induces Autophagy via MYD88 and Strengthens Tight Junction Integrity to Promote the Barrier Function in Intestinal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yumiko Watanabe-Yasuoka,Ayako Gotou,Shigeomi Shimizu,Toshihiro Sashihara

Gut and liver 18:414-425 PubMed36860162

2023

GATA4 Forms a Positive Feedback Loop with CDX2 to Transactivate MUC2 in Bile Acids-Induced Gastric Intestinal Metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Yang,Ting Ye,Li Rong,Hong Peng,Jin Tong,Xiao Xiao,Xiaoqiang Wan,Jinjun Guo

Communications biology 6:85 PubMed36690709

2023

Single-cell profiling reveals differences between human classical adenocarcinoma and mucinous adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Jie Hu,Ying-Jie Li,Li Zhang,Deng-Bo Ji,Xin-Zhi Liu,Yong-Jiu Chen,Lin Wang,Ai-Wen Wu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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