Anti-MUC4 antibody [5B12]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [5B12] (AB60720)

MUC4 antibody (ab60720) used in immunohistochemistry at 3ug/mL on formalin fixed and paraffin embedded human stomach.

This image was generated using the ascites version of the product.

• Flow Cyt

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Flow Cytometry - Anti-MUC4 antibody [5B12] (AB60720)

Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-MUC4 antibody [5B12] (AB60720)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-MUC4 antibody [5B12] (ab60720) at 1 µg/mL

All lanes:

HeLa cell lysate at 25 µg

Predicted band size: 232 kDa

Observed band size: 120 kDa,52 kDa,65 kDa

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• WB

CiteAb

Western blot - Anti-MUC4 antibody [5B12] (AB60720)

MUC4 western blot using anti-MUC4 antibody [5B12] ab60720. Publication image and figure legend from Tang, J., Zhu, Y., et al., 2016, J Exp Clin Cancer Res, PubMed 27287498.

ab60720 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab60720 please see the product overview.

MUC4/Y and MUC4/Y-AMOP domain-deletion constructs and their stable expression in PANC-1 cells. a The structure diagram of the MUC4, MUC4/Y and MUC4/Y-AMOP△. Different domains present in MUC4 protein are the central large tandem repeat domain, NIDO, AMOP, vWD, and 3 carboxyl-terminal located EGF domains. b MUC4/Y expression was analysed by real-time PCR in different PANC-1 derived sub-lines. GAPDH was used as an internal control. c Western blot was conducted to analyse the protein level of MUC4/Y in different groups. GAPDH was probed as an internal control. d Cell confocal immunofluorescence assay showed that the MUC4/Y and the truncated protein (MUC4/Y-AMOPΔ) could anchor on the cytomembrane and cytoplasm.**p < 0.01

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