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Rabbit Recombinant Monoclonal MUC4 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (AB307547), expandable thumbnail
  • Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (AB307547), expandable thumbnail
  • Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (AB307547), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (AB307547), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (AB307547), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.

Alternative names

Mucin-4, MUC-4, Ascites sialoglycoprotein, Pancreatic adenocarcinoma mucin, Testis mucin, Tracheobronchial mucin, ASGP, MUC4

Recommended products

Rabbit Recombinant Monoclonal MUC4 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27199-56
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using 307546, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: skeletal muscle.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    The identity of the bands below 75 kDa are unknown.

    The detected signal is the beta chain of MUC4.

    All lanes: Western blot - Anti-MUC4 antibody [EPR27199-56] (Anti-MUC4 antibody [EPR27199-56] ab307546) at 1/1000 dilution

    Lane 1: Human stomach tissue lysate 20 μg

    Lane 2: Human skeletal muscle cytoplasma tissue lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 80 kDa

    Observed band size: 100 kDa, 170 kDa

    Exposure time: 180s

  • Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Western blot - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using 307546, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Negative control: MIA PaCa-2 (PMID: 21886786), PANC-1 (PMID: 23152882)

    The detected signal is the beta chain of MUC4.

    All lanes: Western blot - Anti-MUC4 antibody [EPR27199-56] (Anti-MUC4 antibody [EPR27199-56] ab307546) at 1/1000 dilution

    Lane 1: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 20 μg

    Lane 2: MIA PaCa-2 (human pancreas epithelial cell) whole cell lysate 20 μg

    Lane 3: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 80 kDa

    Observed band size: 100 kDa, 170 kDa

    Exposure time: 147s

  • Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using Anti-MUC4 antibody [EPR27199-56] ab307546, the same antibody clone in a different buffer formulation.

    MUC4 was immunoprecipitated from 0.35 mg Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-MUC4 antibody [EPR27199-56] ab307546 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MUC4 antibody [EPR27199-56] ab307546 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ABAB307546 IP in Capan-1 whole cell lysate.

    Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ABXXXX in Capan-1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 93 seconds.

    All lanes: Immunoprecipitation - Anti-MUC4 antibody [EPR27199-56] (Anti-MUC4 antibody [EPR27199-56] ab307546) at 1/1000 dilution

    Lane 1: Capan-1 (human pancreas adenocarcinoma epithelial cell) whole cell lysate 10 μg

    Lane 2: Capan-1 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 80 kDa

    Observed band size: 100 kDa, 170 kDa

    Exposure time: 93s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using Anti-MUC4 antibody [EPR27199-56] ab307546, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling MUC4 with Anti-MUC4 antibody [EPR27199-56] ab307546 at 1/500 (0.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on human skeletal muscle. The section was incubated with Anti-MUC4 antibody [EPR27199-56] ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using Anti-MUC4 antibody [EPR27199-56] ab307546, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MUC4 with Anti-MUC4 antibody [EPR27199-56] ab307546 at 1/500 (0.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human colon. The section was incubated with Anti-MUC4 antibody [EPR27199-56] ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC4 antibody [EPR27199-56] - BSA and Azide free (ab307547)

    This data was developed using Anti-MUC4 antibody [EPR27199-56] ab307546, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cervical carci tissue labeling MUC4 with Anti-MUC4 antibody [EPR27199-56] ab307546 at 1/500 (0.96 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on human cervical carcinoma. The section was incubated with Anti-MUC4 antibody [EPR27199-56] ab307546 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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