Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody that is used to detect MUC5B in Western Blot, IHC-P, IHC-Fr, ELISA. Suitable for Human, Monkey, Mouse samples.
- Over 20 publications
- Trusted since 2009
Images
![Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995), expandable thumbnail](https://content.abcam.com/products/images/muc5b-antibody-194e-ab77995--immunohistochemistry-frozen-sections-img82347.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)")
Publications
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- Frontiers in pharmacology 13:8513742022Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Iwashita,Hikari Maeda,Momo Ishimura,Jun MurataPubMed 36188610
- iScience 25:1046852022Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDinesh Devadoss,Arpan Acharya,Marko Manevski,Dominika Houserova,Michael D Cioffi,Kabita Pandey,Madhavan Nair,Prem Chapagain,Mehdi Mirsaeidi,Glen M Borchert,Siddappa N Byrareddy,Hitendra S ChandPubMed 35789750
- Scientific reports 12:87422022The normal and fibrotic mouse lung classified by spatial proteomic analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRoberta Ciccimarra,Maddalena M Bolognesi,Matteo Zoboli,Giorgio Cattoretti,Franco F Stellari,Francesca RavanettiPubMed 35610327
- Nature protocols 17:1658-16902022Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRajendra Kumar Gurumurthy,Stefanie Koster,Naveen Kumar,Thomas F Meyer,Cindrilla ChumduriPubMed 35546639
- Respiratory research 23:112022MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuan Huang,Weijie Guan,Bin Xiang,Wei Wang,Yanqing Xie,Jinping ZhengPubMed 35042537
- Cellular & molecular immunology 19:210-2212022SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenfeng Wang,Jiadi Lv,Pin Yu,Yajin Qu,Yabo Zhou,Li Zhou,Qiangqiang Zhu,Shunshun Li,Jiangping Song,Wei Deng,Ran Gao,Yuying Liu,Jiangning Liu,Wei-Min Tong,Chuan Qin,Bo HuangPubMed 34983944
- Biochemistry and biophysics reports 28:1011242021Integrin β1 subunit regulates cellular and secreted MUC5AC and MUC5B production in NCI-H292 human lung epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Iwashita,Jun MurataPubMed 34504957
- Nature nanotechnology 16:942-9512021Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhua Li,Zhenzhen Wang,Phuong-Uyen C Dinh,Dashuai Zhu,Kristen D Popowski,Halle Lutz,Shiqi Hu,Mark G Lewis,Anthony Cook,Hanne Andersen,Jack Greenhouse,Laurent Pessaint,Leonard J Lobo,Ke ChengPubMed 34140674
- Respiratory research 22:1722021Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKristina Andelid,Karolina Öst,Anders Andersson,Esha Mohamed,Zala Jevnikar,Lowie E G W Vanfleteren,Melker GöranssonPubMed 34098956
- Cell discovery 7:242021Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiadi Lv,Zhenfeng Wang,Yajin Qu,Hua Zhu,Qiangqiang Zhu,Wei Tong,Linlin Bao,Qi Lv,Ji Cong,Dan Li,Wei Deng,Pin Yu,Jiangping Song,Wei-Min Tong,Jiangning Liu,Yuying Liu,Chuan Qin,Bo HuangPubMed 33850112
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human MUC5B. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC84
Reactivity data
Select an application| ELISA | WB | IHC-P | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Expected | Expected | Predicted |
| Mouse | Expected | Expected | Expected | Tested |
| Monkey | Expected | Expected | Expected | Predicted |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Deglycosylating the tissue is critical to expose epitope. |
Species Monkey | Dilution info - | Notes Deglycosylating the tissue is critical to expose epitope. |
Species Human | Dilution info - | Notes Deglycosylating the tissue is critical to expose epitope. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Alternative names
MUC5, MUC5B, Mucin-5B, MUC-5B, Cervical mucin, High molecular weight salivary mucin MG1, Sublingual gland mucin
Recommended products
Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody that is used to detect MUC5B in Western Blot, IHC-P, IHC-Fr, ELISA. Suitable for Human, Monkey, Mouse samples.
- Over 20 publications
- Trusted since 2009
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human MUC5B. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC84
- Clone number
- 19.4E
- Purification technique
- Affinity purification Protein A
- Specificity
Does not react with human Mucin 5A or 5C.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Monkey, Mouse samples.
Trusted by the scientific community
Anti-MUC5B [19.4E] (ab77995) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (ab77995), expandable thumbnail](https://content.abcam.com/products/images/muc5b-antibody-194e-ab77995--immunohistochemistry-frozen-sections-img82347.jpg "Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (ab77995)")
Image courtesy of Dr Francois Daubeuf by customer review.Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (ab77995)
ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4μg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).
This image was generated using the ascites version of the product.
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