AB77995

Anti-MUC5B antibody [19.4E]

Name: Anti-MUC5B antibody [19.4E] (ab77995) | Abcam
Brand: Abcam Limited
SKU: AB77995
Rating: 5 (2 reviews)

(2 Reviews)

(30 Publications)

Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody detecting MUC5B in Western Blot, IHC-P, IHC-Fr, ELISA. Suitable for Human, Monkey, Mouse.

- Over 20 publications
- Trusted since 2009

View Alternative Names

MUC5, MUC5B, Mucin-5B, MUC-5B, Cervical mucin, High molecular weight salivary mucin MG1, Sublingual gland mucin

2 Images

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4μg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).

This image was generated using the ascites version of the product.

Image courtesy of Dr Francois Daubeuf by Abreview.

CiteAb

Western blot - Anti-MUC5B antibody [19.4E] (AB77995)

Western Blotting using Anti-MUC5B antibody [19.4E], ab77995. Publication image from Liu, Y. et al., 2020, Cell Res, 33159154. Legend direct from paper.

IFN-β and IFN-γ activated the Kyn-AhR pathway for mucin expression.a PAECs or BEAS-2B cells were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 48 h. The expression of IDO1 was determined by western blotting. b BEAS-2B cells were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 48 h. The levels of Kyn from cell lysates or the supernatants were determined by ELISA. c PAECs were isolated from AhR–/– mice were treated with IFN-β (1 ng/mL) or IFN-γ (10 ng/mL) for 24 h. The expression of Mucs 1, 2, 5A, 6, 13, 15 and 16 was determined by real-time PCR. d BEAS-2B cells were treated with Kyn (400 µM) for 24 h. The expression of mucins (MUCs 2, 5A, 6, 7, 13, 15, 16, 17, 18 and 20) was determined by real-time PCR. e Western blot analysis of the expression of mucins 1, 5A, 5B, 13 and 16 from BEAS-2B cells or PAECs treated with Kyn (400 µM) for 48 h. The data represent means ± SD. In b–d, n = 3 biological independent samples. Representative images are from three independent experiments (a and e). *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student’s t-test (d) or one-way ANOVA (b and c).

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

19.4E

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human, Monkey

Applications

ELISA, IHC-Fr, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human MUC5B. The exact immunogen used to generate this antibody is proprietary information.

Q9HC84

Specificity

Does not react with human Mucin 5A or 5C.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Deglycosylating the tissue is critical to expose epitope.", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-MUC5B antibody [19.4E] (ab77995) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Monkey, Mouse samples.

Trusted by the scientific community
Anti-MUC5B [19.4E] (ab77995) was first used in a scientific publication in 2009 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.

See full target information MUC5B

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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869396

2025

Liquiritin Suppresses Intracellular and Secreted MUC5AC and MUC5B in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoma Yoshio,Jun Iwashita

ImmunoTargets and therapy 13:789-803 PubMed39712952

2024

Lipopolysaccharide in Bile Promotes the Neutrophil Extracellular Traps-Induced Gallstone Formation by Activating the Gallbladder Immune Barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Yu,Ziang Meng,Xuxu Liu,Yi Zheng,Dongbo Xue,Chenjun Hao,Liyi Wang

International journal of chronic obstructive pulmonary disease 19:1635-1647 PubMed39045541

2024

Cigarette Smoke Extract Induces MUC5AC Expression Through the ROS/ IP3R/Ca Pathway in Calu-3 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wu,Guoyue Zhang,Xianzhi Du

International journal of molecular medicine 53: PubMed38240085

2024

NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Zhou,Qun Zhang,Chenyang Liu,Jiahao Fan,Wen Huang,Nan Li,Mingxia Yang,Hong Wang,Weiping Xie,Hui Kong

Nature cardiovascular research 2:449-466 PubMed39196043

2023

Three-dimensional morphologic and molecular atlases of nasal vasculature.

Applications

Unspecified application

Species

Unspecified reactive species

Seon Pyo Hong,Myung Jin Yang,Jung Hyun Bae,Du Ri Choi,Young-Chan Kim,Myeon-Sik Yang,Byungkwan Oh,Kyung Won Kang,Sang-Myeong Lee,Bumseok Kim,Yong-Dae Kim,Ji Hoon Ahn,Gou Young Koh

Frontiers in pharmacology 13:851374 PubMed36188610

2022

Type IV collagen reduces MUC5AC secretion in the lungs of ovalbumin-sensitized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Iwashita,Hikari Maeda,Momo Ishimura,Jun Murata

iScience 25:104685 PubMed35789750

2022

Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Dinesh Devadoss,Arpan Acharya,Marko Manevski,Dominika Houserova,Michael D Cioffi,Kabita Pandey,Madhavan Nair,Prem Chapagain,Mehdi Mirsaeidi,Glen M Borchert,Siddappa N Byrareddy,Hitendra S Chand

Scientific reports 12:8742 PubMed35610327

2022

The normal and fibrotic mouse lung classified by spatial proteomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Ciccimarra,Maddalena M Bolognesi,Matteo Zoboli,Giorgio Cattoretti,Franco F Stellari,Francesca Ravanetti

Nature protocols 17:1658-1690 PubMed35546639

2022

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.

Applications

Unspecified application

Species

Unspecified reactive species

Rajendra Kumar Gurumurthy,Stefanie Koster,Naveen Kumar,Thomas F Meyer,Cindrilla Chumduri

Respiratory research 23:11 PubMed35042537

2022

MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Huang,Weijie Guan,Bin Xiang,Wei Wang,Yanqing Xie,Jinping Zheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MUC5B antibody [19.4E]

Immunohistochemistry (Frozen sections) - Anti-MUC5B antibody [19.4E] (AB77995)

Western blot - Anti-MUC5B antibody [19.4E] (AB77995)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (30)

Product promise