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AB317125

Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026]

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Rabbit Monoclonal antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MUC4.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)

ab317125 detects Mucin 4 X + Mucin 4 Y protein at cell membrane by immunofluorescent analysis.
Sample : NCI-H441 cells were fixed in ice-cold MeOH for 5 min.
Green : Mucin 4 X + Mucin 4 Y stained by ab317125 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)
  • WB

Supplier Data

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317125 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (ab317125) at 1/1000 dilution

Lane 1:

NCI-H441 whole cell extracts at 30 µg

Lane 2:

CFPAC-1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)
  • WB

Supplier Data

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)

Various tissue extracts (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317125 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (ab317125) at 1/1000 dilution

Lane 1:

Human colon tissue extracts at 30 µg

Lane 2:

Human liver tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)
  • WB

Supplier Data

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (AB317125)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE and the membrane was blotted with ab317125 diluted at 1 : 100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Mucin 4 X + Mucin 4 Y antibody [HL2026] (ab317125) at 1/100000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

GFP-tagged Munc4 X transfected (+) 293T whole cell extracts

Lane 3:

GFP-tagged Munc4 Y transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2026

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MUC4.

Q99102

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Membrane-bound mucin, a family of highly glycosylated proteins that constitute the major component of the mucus, the slimy and viscous secretion covering epithelial surfaces (PubMed:10880978). These glycoproteins play important roles in the protection of the epithelium and are implicated in epithelial renewal and differentiation (PubMed:10880978). Regulates cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and its ability to act as an intramembrane ligand for ERBB2. Plays an important role in proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. In polarized epithelial cells, segregates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. Its ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation.

Additional targets

MUC4
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Product promise

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