AB24071

Anti-Mucin 5AC antibody [2-11M1]

Name: Anti-Mucin 5AC antibody [2-11M1] (ab24071) | Abcam
Brand: Abcam Limited
SKU: AB24071

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(27 Publications)

Mouse Monoclonal Mucin 5AC antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 27 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human MUC5AC.

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

1 Images

Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (AB24071)

Flow Cyt

Unknown

Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (AB24071)

Overlay histogram showing A549 cells stained with ab24071 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab24071, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2-11M1

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human MUC5AC.

P98088

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Reactivity data

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Product details

This antibody reacts with peptide core of gastric mucin restricted to the surface gastric epithelium. It characterised the M1-b epitope, completely destroyed by b-mercapto-ethano. The M1-b epitope is found located on a 58 kD and 40 kD peptide after trypsin treatment of native M1 mucin. This M1-b epitope coprecipites with the M1-f epitope encoded by Mucin 5AC gene. It is probably associated with the gastric mucin encoded by Mucin 5AC gene or with a gastric mucin encoded by another gastric MUC gene which polymerized with the M1/Mucin 5AC gastric mucin.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).

See full target information MUC5AC

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Publications (27)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 325:L689-L708 PubMed37642665

2023

Caveolin-1-derived peptide attenuates cigarette smoke-induced airway and alveolar epithelial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Durgesh Nandini Das,Bijesh Puthusseri,Venkadesaperumal Gopu,Venugopal Krishnan,Ashoka Kumar Bhagavath,Sudhir Bolla,Yogesh Saini,Gerald J Criner,Nathaniel Marchetti,Hua Tang,Nagarjun V Konduru,Liang Fan,Sreerama Shetty

Dose-response : a publication of International Hormesis Society 20:15593258221117349 PubMed36003321

2022

Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Wol Soon Jo,Sohi Kang,Soo Kyung Jeong,Min Ji Bae,Chang Geun Lee,Yeonghoon Son,Hae-June Lee,Min Ho Jeong,Sung Ho Kim,Chongjong Moon,In Sik Shin,Joong Sun Kim

Frontiers in physiology 13:884166 PubMed35574458

2022

Muc5b Contributes to Mucus Abnormality in Rat Models of Cystic Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Johnathan D Keith,Alexander G Henderson,Courtney M Fernandez-Petty,Joy M Davis,Ashley M Oden,Susan E Birket

Translational cancer research 10:4047-4056 PubMed35116702

2022

Tumor necrosis factor-α promotes airway mucus hypersecretion by repressing miR-146a-5p and miR-134-5p levels in human airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Ting Fu,Yan Zhang,Ping Zhang,Huan Wu,Xuan-Qiu Sun,Shu-Yang Shen,Dan-Bo Dou

Scientific reports 12:1540 PubMed35087167

2022

A medium composition containing normal resting glucose that supports differentiation of primary human airway cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel Morgan,Candela Manfredi,Kristen F Easley,Lionel D Watkins,William R Hunt,Steven L Goudy,Eric J Sorscher,Michael Koval,Samuel A Molina

Respiratory research 23:11 PubMed35042537

2022

MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Huang,Weijie Guan,Bin Xiang,Wei Wang,Yanqing Xie,Jinping Zheng

Cellular & molecular immunology 19:210-221 PubMed34983944

2022

SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfeng Wang,Jiadi Lv,Pin Yu,Yajin Qu,Yabo Zhou,Li Zhou,Qiangqiang Zhu,Shunshun Li,Jiangping Song,Wei Deng,Ran Gao,Yuying Liu,Jiangning Liu,Wei-Min Tong,Chuan Qin,Bo Huang

Cell discovery 7:24 PubMed33850112

2021

Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Jiadi Lv,Zhenfeng Wang,Yajin Qu,Hua Zhu,Qiangqiang Zhu,Wei Tong,Linlin Bao,Qi Lv,Ji Cong,Dan Li,Wei Deng,Pin Yu,Jiangping Song,Wei-Min Tong,Jiangning Liu,Yuying Liu,Chuan Qin,Bo Huang

Respiratory research 22:65 PubMed33622325

2021

Olodaterol exerts anti-inflammatory effects on COPD airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Yang,Gurpreet K Singhera,Yi Xuan Yan,Michael P Pieper,Janice M Leung,Don D Sin,Delbert R Dorscheid

Cell research 30:1078-1087 PubMed33159154

2020

Mucus production stimulated by IFN-AhR signaling triggers hypoxia of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Liu,Jiadi Lv,Jiangning Liu,Man Li,Jing Xie,Qi Lv,Wei Deng,Nannan Zhou,Yabo Zhou,Jiangping Song,Peng Wang,Chuan Qin,Wei-Min Tong,Bo Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mucin 5AC antibody [2-11M1]

Flow Cytometry - Anti-Mucin 5AC antibody [2-11M1] (AB24071)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (27)

Product promise