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Anti-Mucin 5AC antibody 45M1 is a mouse monoclonal antibody that is used in Mucin 5AC IHC, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Recombinant format ensures unrivaled batch-to-batch consistency
- Cited in over 145 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (AB3649), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (AB3649), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (AB3649), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (AB3649), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (AB3649), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PmIHCIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Tested
Expected
Tested
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/5000.00000
Notes

-

Species
Rat
Dilution info
1/100.00000 - 1/5000.00000
Notes

-

Species
Human
Dilution info
1/100.00000 - 1/5000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

11 products for Alternative Product

Target data

Function

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).

Alternative names

Recommended products

Anti-Mucin 5AC antibody 45M1 is a mouse monoclonal antibody that is used in Mucin 5AC IHC, ICC/IF. Suitable for Human, Mouse, Rat samples.

- Recombinant format ensures unrivaled batch-to-batch consistency
- Cited in over 145 publications

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
45M1
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product has switched from a hybridoma to recombinant production method on 8th March 2021.

Product Specifications
Anti-Mucin 5AC antibody [45M1] (ab3649) is a mouse recombinant monoclonal antibody and is validated for use in ICC/IF, IHC-Fr, IHC-P, mIHC in human, mouse, rat samples.
Anti-Mucin 5AC antibody [45M1] (ab3649) specifically detects Mucin 5AC (UniProt ID: P98088; Molecular weight: 583kDa) and is sold in a convenient trial size to enable initial testing (20 µg) and larger sizes for subsequent scaling up experiments (100 µg and 1 mg).

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Mucin 5AC antibody [45M1] (ab3649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Mucin 5AC antibody [45M1] (ab3649) has been cited over 146 times in peer reviewed journals and is trusted by the scientific community.
Anti-Mucin 5AC antibody [45M1] (ab3649) has 6 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 45M1 - Anti-PCNA antibody [PC10] - BSA and Azide free ab264494.
Antibody clone 45M1 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (Alexa Fluor® 488 Anti-Mucin 5AC antibody [45M1] ab309610, Alexa Fluor® 647 Anti-Mucin 5AC antibody [45M1] ab309611).

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail
    Image from Kikuchi M et al., BMC Gastroenterol. 2010 Jun 18;10:65. doi: 10.1186/1471-230X-10-65.; Fig 5.; 18 June 2010, BMC Gastroenterology 2010, 10:65

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    This image was generated from the hybridoma version of the product.

    Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.

    Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.

  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunocytochemistry analysis of A549 (human lung carcinoma cell line) cells labeling Mucin 5AC with ab3649 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (Anti-beta Tubulin antibody [EPR16774] ab179513) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    Negative control 1: ab3649 (Mouse monoclonal antibody to Mucin 5AC at 1/100 dilution) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 (anti-Rabbit secondary Alexa Fluor® 594 at 1/1000 dilution)

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 (Rabbit monoclonal antibody to beta Tubulin at 1/200 dilution) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 (anti-Mouse secondary Alexa Fluor® 488 at 1/1000 dilution)

  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemistry (Frozen sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Multiplex immunohistochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (ab3649, gray; Opal™690), anti-MUC-6 (Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab3649 (1/5000 dilution), Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846 (1/1000 dilution), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

  • Immunocytochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunocytochemistry analysis of paraformaldehyde-fixed 0.5% Triton in PBS-permeabilized human HBE cells staining with ab3649 at 1/300 dilution. Secondary antibody was Alexa Fluor® 594 Goat Thermo A-11005 at 1/250 dilution. Red: Actin Magenta: MUC5AC Blue: DNA. Samples were incubated with the primary antibody with 1% BSA in PBS for 16 hours at 4°C.

  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Calu-3 (human lung adenocarcinoma epithelial cell) labelling (A) MUC5B with Anti-MUC5B antibody [EPR27200-159] ab315330 at 1/500 (1.008 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green), or (B) MUC5AC with ab3649 at 1/100 (10.45 ug/ml) followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    This antibody does not cross-react with human MUC5AC.

    (A) No staining of MUC5B in Calu-3 cell line.

    (B) Positive staining of MUC5AC (ab3649, green) in Calu-3 cell line.

    Images were taken with a confocal microscope (Leica-Microsystems, TCS SP8).
    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemical analysis of paraffin-embedded (A, C) A-549 (human lung carcinoma epithelial cell) cell pellet (B, D) Calu-3 (human lung adenocarcinoma epithelial cell) cell pellets.

    A & B: whole cell pellet labelling of Mucin 5B with Anti-MUC5B antibody [EPR27200-159] ab315330 at 1/100 dilution (5.04 ug/ml). C & D: whole cell pellet labelling of Mucin 5A with ab3649 at 1/5000 dilution (0.211 ug/ml).

    This antibody recognizes human MUC5B but not human MUC5AC.

    Positive staining on (A) A-549 cell pellet. No staining on (B) Calu-3 cell pellet. The sections were incubated with Anti-MUC5B antibody [EPR27200-159] ab315330 or ab3649 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

    Immunohistochemical analysis of formalin fixed paraffin embedded human stomach labelling Mucin 5AC with ab3649 at a concentration of 0.05µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    Anti-Mucin 5AC antibody [45M1] ab3649 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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