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Mouse Recombinant Monoclonal Mucin 5AC antibody. Carrier free. Suitable for mIHC, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 11 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (AB212636), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PICC/IFIHC-Fr
Human
Tested
Tested
Tested
Expected
Mouse
Expected
Tested
Expected
Tested
Rat
Expected
Tested
Expected
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

1 products for Alternative Version

Target data

Function

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).

Alternative names

Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM, MUC5AC, MUC5

Recommended products

Mouse Recombinant Monoclonal Mucin 5AC antibody. Carrier free. Suitable for mIHC, IHC-P, ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

Alternative names

Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM, MUC5AC, MUC5

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

45M1

Purification technique

Affinity purification Protein A

Epitope

Located in the C-terminal cysteine rich part of the peptide core of MUC5AC.

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

This product has switched from a hybridoma to recombinant production method on 8th March 2021.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

Associated diseases and disorders

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with Anti-Mucin 5AC antibody [45M1] ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunocytochemistry analysis of A549 (human lung carcinoma cell line) cells labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (Anti-beta Tubulin antibody [EPR16774] ab179513) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

    Negative control 1: Anti-Mucin 5AC antibody [45M1] ab3649 (Mouse monoclonal antibody to Mucin 5AC at 1/100 dilution) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 (anti-Rabbit secondary Alexa Fluor® 594 at 1/1000 dilution)

    Negative control 2: Anti-beta Tubulin antibody [EPR16774] ab179513 (Rabbit monoclonal antibody to beta Tubulin at 1/200 dilution) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 (anti-Mouse secondary Alexa Fluor® 488 at 1/1000 dilution)

  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with Anti-Mucin 5AC antibody [45M1] ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Frozen sections) analysis of rat stomach tissue labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labeling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with Anti-Mucin 5AC antibody [45M1] ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Multiplex immunohistochemistry - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (Anti-Mucin 5AC antibody [45M1] ab3649, gray; Opal™690), anti-MUC-6 (Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846, green; Opal™520) and anti-Rab3D (Anti-Rab3D antibody [EPR8106] ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Mucin 5AC antibody [45M1] ab3649 (1/5000 dilution), Anti-Gastric Mucin/MUC-6 antibody [EPR20623] ab223846 (1/1000 dilution), and Anti-Rab3D antibody [EPR8106] ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] - BSA and Azide free (ab212636)

    This data was developed using Anti-Mucin 5AC antibody [45M1] ab3649, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of formalin fixed paraffin embedded human stomach labelling Mucin 5AC with Anti-Mucin 5AC antibody [45M1] ab3649 at a concentration of 0.05µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    Anti-Mucin 5AC antibody [45M1] Anti-Mucin 5AC antibody [45M1] ab3649 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

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