AB198294

Anti-Mucin 5AC antibody [EPR16904]

RabMAb
Recombinant
What is this?

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(34 Publications)

Anti-Mucin 5AC antibody [EPR16904] (ab198294) is a rabbit monoclonal antibody detecting Mucin 5AC in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Mucin 5AC with ab198294 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasm staining on HeLa cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

The negative controls are as follows : -
-ve control 1 : ab198294 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Mucin 5AC with ab198294 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).

Cytoplasm staining on SW480 cell line is observed.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Mucin 5AC with ab198294 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on Human stomach tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Blocking/Dilution buffer : 5% NFDM/TBST.

This protein is highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. The expression profile observed is consistent with what has been described in the literature PMID : 15050369.

All lanes:

Western blot - Anti-Mucin 5AC antibody [EPR16904] (ab198294) at 1/20000 dilution

Lane 1:

Human stomach lysate at 20 µg

Lane 2:

Human colon cancer lysate at 20 µg

Lane 3:

Human small intestine lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 586 kDa

Observed band size: 250-600 kDa

false

Exposure time: 5s

617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Mucin 5AC antibody [EPR16904]
HRP

HRP Anti-Mucin 5AC antibody [EPR16904]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904]
Carrier free

Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free
Carrier free

Anti-Mucin 5AC antibody [EPR16904] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16904

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-Mucin 5AC antibody [EPR16904] (ab198294) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Mucin 5AC?
Anti-Mucin 5AC [EPR16904] (ab198294) specifically detects a band for Mucin 5AC (UniProt: P98088) at a molecular weight of 527kDa.

Trusted by the scientific community
Anti-Mucin 5AC [EPR16904] (ab198294) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR16904] also available for your convenience: ab198294, Alexa Fluor® 594 - ab218363, HRP - ab218713, Alexa Fluor® 555 - ab218714, Carrier free - ab229451, Carrier free - ab271960

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed : 14535999, PubMed : 14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed : 14535999).

See full target information MUC5AC

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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40801617

2025

Structure of the Secretory Compartments in Goblet Cells in the Colon and Small Intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander A Mironov,Irina S Sesorova,Pavel S Vavilov,Roberto Longoni,Paola Briata,Roberto Gherzi,Galina V Beznoussenko

Journal of biomedical science 32:63 PubMed40634947

2025

Goblet cell breakdown: transcriptomics reveals Acinetobacter baumannii early and robust inflammatory response in differentiated human bronchial epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Scribano,Claudia Tito,Astri D Tagueha,Martina Pasqua,Luciana De Angelis,Francesco Fazi,Dolores Limongi,Marta De Angelis,Lucia Nencioni,Anna Teresa Palamara,Cecilia Ambrosi

Frontiers in immunology 16:1532144 PubMed39981254

2025

Comparison of air-liquid interface transwell and airway organoid models for human respiratory virus infection studies.

Applications

Unspecified application

Species

Unspecified reactive species

Camilla T Ekanger,Nilima Dinesh Kumar,Rosanne W Koutstaal,Fan Zhou,Martin Beukema,Joanna Waldock,Simon P Jochems,Noa Mulder,Cécile A C M van Els,Othmar G Engelhardt,Nathalie Mantel,Kevin P Buno,Karl Albert Brokstad,Agnete S T Engelsen,Rebecca J Cox,Barbro N Melgert,Anke L W Huckriede,Puck B van Kasteren

Cell & bioscience 15:18 PubMed39920853

2025

Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhu,Wenhao Yang,Jiaxin Luo,Danli Lu,Yanan Hu,Rui Zhang,Yan Li,Li Qiu,Zelian Chen,Lina Chen,Hanmin Liu

International journal of biological sciences 21:17-39 PubMed39744426

2025

Advanced Lung-on-a-Chip Technology: Mimicking the Complex Human Lung Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Kyung Min,Choon-Mi Lee,Soo-Rim Kim,Jin Woo Lee,Chan Hum Park,Byung-Chul Oh,YunJae Jung,Hwa-Yong Lee

Biology direct 19:114 PubMed39533404

2024

DEPTOR attenuates asthma progression by suppressing endoplasmic reticulum stress through SOD1.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Lei Zhang,Yunxiao Shang

BMJ open respiratory research 11: PubMed39521608

2024

Pim1 inactivating induces RUNX3 upregulation that improves/alleviates airway inflammation and mucus hypersecretion in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Fang,Zhen Guo,Lanzhi Zhou,Juan Zhang,Haiyan Li,Jumei Hao

Theranostics 14:6185-6201 PubMed39431015

2024

Spatial transcriptomic sequencing reveals immune microenvironment features of granulomas in lung and omentum.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochen Qiu,Pengfei Zhong,Liang Yue,Chaofan Li,Zhimin Yun,Guangqian Si,Mengfan Li,Zhi Chen,Yingxia Tan,Pengtao Bao

Stem cell research & therapy 15:286 PubMed39256871

2024

Connexin 25 maintains self-renewal and functions of airway basal cells for airway regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyuan Zhang,Shaoyang Wang,Zeyu Liu,Cheng Zhong,Yuqiong Lei,Qi Zheng,Yongle Xu,Shan Shan,Hao He,Tao Ren

PloS one 19:e0306058 PubMed38935605

2024

Assessment of polymeric mucin-drug interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Hisanao Kishimoto,Caroline Ridley,Katsuhisa Inoue,David J Thornton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mucin 5AC antibody [EPR16904]

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

Western blot - Anti-Mucin 5AC antibody [EPR16904] (AB198294)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (34)

Product promise