Anti-Mucin 5AC antibody [EPR16904] (ab198294) is a rabbit monoclonal antibody that is used to detect Mucin 5AC in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (AB198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44319.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (AB198294)")
![Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunocytochemistry-immunofluorescence-img76840.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)")
![Western blot - Anti-Mucin 5AC antibody [EPR16904] (AB198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--western-blot-img107580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Mucin 5AC antibody [EPR16904] (AB198294)")
![Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunocytochemistry-immunofluorescence-img69465.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (AB198294)")
Publications
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- Children (Basel, Switzerland) 10:2023Airway Epithelial Cultures of Children with Esophageal Atresia as a Model to Study Respiratory Tract Disorders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHenriette H M Dreyer,Eleonora Sofie van Tuyll van Serooskerken,Lisa W Rodenburg,Arnold J N Bittermann,Hubertus G M Arets,Ellen M B P Reuling,Johannes W Verweij,Eric G Haarman,David C van der Zee,Stefaan H A J Tytgat,Cornelis K van der Ent,Jeffrey M Beekman,Gimano D Amatngalim,Maud Y A LindeboomPubMed 37371252
- Experimental and therapeutic medicine 25:2392023A novel type of serum‑free medium for culturing human airway epithelium cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaotian Dai,Qi Zheng,Shan Shan,Yuqiong Lei,Jingyuan Zhang,Cheng Zhong,Yongle Xu,Zeyu Liu,Tao RenPubMed 37114176
- Life science alliance 5:2022Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGimano D Amatngalim,Lisa W Rodenburg,Bente L Aalbers,Henriette Hm Raeven,Ellen M Aarts,Dounia Sarhane,Sacha Spelier,Juliet W Lefferts,Iris Al Silva,Wilco Nijenhuis,Sacha Vrendenbarg,Evelien Kruisselbrink,Jesse E Brunsveld,Cornelis M van Drunen,Sabine Michel,Karin M de Winter-de Groot,Harry G Heijerman,Lukas C Kapitein,Magarida D Amaral,Cornelis K van der Ent,Jeffrey M BeekmanPubMed 35922154
- Journal of inflammation research 15:3661-36752022Moderate Dose Irradiation Induces DNA Damage and Impairments of Barrier and Host Defense in Nasal Epithelial Cells in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue-Ying Yang,Jing Liu,Yi-Tong Liu,Hsiao-Hui Ong,Qian-Min Chen,Ce-Belle Chen,Mark Thong,Xinni Xu,Sui-Zi Zhou,Qian-Hui Qiu,De-Yun WangPubMed 35783248
- Nature communications 13:13092022Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Hong,Shan Shan,Ye Gu,Haidong Huang,Quncheng Zhang,Yang Han,Yongpin Dong,Zeyu Liu,Moli Huang,Tao RenPubMed 35288560
- Bioengineered 13:1198-12082022Taxifolin improves inflammatory injury of human bronchial epithelial cells by inhibiting matrix metalloproteinase (MMP) 10 via Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouhua Chen,Yan Mei,Lu Yang,Weibin Li,Yu Zhou,Surong He,Jie LiangPubMed 35000533
- mSphere 6:e00848212021NanI Sialidase Enhances the Action of Clostridium perfringens Enterotoxin in the Presence of Mucus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMauricio A Navarro,Jihong Li,Juliann Beingesser,Bruce A McClane,Francisco A UzalPubMed 34908460
- Nucleic acids research 49:12502-125162021Inhibition of SARS-CoV-2 coronavirus proliferation by designer antisense-circRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristina Pfafenrot,Tim Schneider,Christin Müller,Lee-Hsueh Hung,Silke Schreiner,John Ziebuhr,Albrecht BindereifPubMed 34850109
- Molecular medicine reports 24:2021Formononetin ameliorates IL‑13‑induced inflammation and mucus formation in human nasal epithelial cells by activating the SIRT1/Nrf2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuanjuan Huang,Xianfeng Chen,Aihua XiePubMed 34590155
- International journal of molecular sciences 22:2021Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara I Van Acker,Bert Van den Bogerd,Zoë P Van Acker,Agnė Vailionytė,Michel Haagdorens,Carina Koppen,Sorcha Ní Dhubhghaill,Darlene A Dartt,Isabel PintelonPubMed 34638869
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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1 product for Alternative Version
Target data
Function
Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Alternative names
MUC5, MUC5AC, Mucin-5AC, MUC-5AC, Gastric mucin, Major airway glycoprotein, Tracheobronchial mucin, TBM
Recommended products
Anti-Mucin 5AC antibody [EPR16904] (ab198294) is a rabbit monoclonal antibody that is used to detect Mucin 5AC in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 10 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR16904
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Mucin 5AC antibody [EPR16904] (ab198294) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.
What is the molecular weight of Mucin 5AC?
Anti-Mucin 5AC [EPR16904] (ab198294) specifically detects a band for Mucin 5AC (UniProt: P98088) at a molecular weight of 527kDa.
Recommended positive controls
WB: Human stomach lysate.IHC-P: Human stomach tissue.ICC/IF: HeLa and SW480 cells.
Trusted by the scientific community
Anti-Mucin 5AC [EPR16904] (ab198294) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products
We have a range of other formats of antibody clone [EPR16904] also available for your convenience:
ab198294, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Mucin 5AC antibody [EPR16904] ab218363, HRP - HRP Anti-Mucin 5AC antibody [EPR16904] ab218713, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Mucin 5AC antibody [EPR16904] ab218714, Carrier free - Anti-Mucin 5AC antibody [EPR16904] - Low endotoxin, Azide free ab229451, Carrier free - Anti-Mucin 5AC antibody [EPR16904] - BSA and Azide free ab271960
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.
- Biological function summary
The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.
- Pathways
The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.
- Associated diseases and disorders
MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (ab198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44319.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (ab198294)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [EPR16904] (ab198294)
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Mucin 5AC with ab198294 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Cytoplasm staining on Human stomach tissue is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunocytochemistry-immunofluorescence-img76840.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294)")
Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Mucin 5AC with ab198294 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on HeLa cell line is observed.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198294 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Western blot - Anti-Mucin 5AC antibody [EPR16904] (ab198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--western-blot-img107580.jpg "Western blot - Anti-Mucin 5AC antibody [EPR16904] (ab198294)")
Western blot - Anti-Mucin 5AC antibody [EPR16904] (ab198294)
Blocking/Dilution buffer: 5% NFDM/TBST.
This protein is highly expressed in surface mucosal cells of respiratory tract and stomach epithelia. The expression profile observed is consistent with what has been described in the literature PMID: 15050369.
All lanes: Western blot - Anti-Mucin 5AC antibody [EPR16904] (ab198294) at 1/20000 dilution
Lane 1: Human stomach lysate at 20 µg
Lane 2: Human colon cancer lysate at 20 µg
Lane 3: Human small intestine lysate at 20 µg
Lane 4: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 586 kDa
Observed band size: 250-600 kDa
Exposure time: 5s
![Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294), expandable thumbnail](https://content.abcam.com/products/images/mucin-5ac-antibody-epr16904-ab198294--immunocytochemistry-immunofluorescence-img69465.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294)")
Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [EPR16904] (ab198294)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Mucin 5AC with ab198294 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on SW480 cell line is observed.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1: ab198294 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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