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Mouse Monoclonal Mucin 5AC antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MUC5AC.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [MUC5AC/917] (AB218466), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human MUC5AC. The exact immunogen used to generate this antibody is proprietary information. Database link P98088

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Rat
Not recommended

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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1 product for Alternative Version

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Target data

Function

Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).

Alternative names

Recommended products

Mouse Monoclonal Mucin 5AC antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MUC5AC.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human MUC5AC. The exact immunogen used to generate this antibody is proprietary information. Database link P98088
Clone number

MUC5AC/917

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...
Purification notes

ab218466 is purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Mucin 5AC also known simply as MUC5AC is a high molecular weight glycoprotein with a mass in the megadaltons typically found in the human respiratory gastrointestinal and reproductive tracts. It is a major component of mucus which plays a critical role in protecting and lubricating the epithelial surfaces of these organs. MUC5AC is primarily expressed in the goblet cells of the respiratory epithelium and the stomach lining. Due to its significance researchers often use a MUC5AC ELISA or other detection methods such as tagging with Alexa Fluor 555 to measure its levels in various tissues.

Biological function summary

The functions of MUC5AC are central to the maintenance of the protective mucus barrier on epithelial surfaces preventing pathogen invasion and facilitating the clearance of inhaled particles. It is a part of the gel-forming mucin family and often forms complex structures by polymerizing with other mucins like MUC2 and MUC5B contributing to the viscoelastic properties of mucus. These interactions ensure the formation of a stable and robust mucosal barrier.

Pathways

The regulation of MUC5AC production involves several signaling pathways including the EGFR (epidermal growth factor receptor) pathway and the IL-13/STAT6 pathway. These pathways influence mucin production and secretion particularly in response to growth factors and inflammatory cytokines. Proteins such as 13M1 and 11M1 interact with MUC5AC within these pathways modulating its expression and function. This regulation is essential for normal mucus homeostasis and response to environmental challenges.

Associated diseases and disorders

MUC5AC has a strong association with respiratory conditions like asthma and chronic obstructive pulmonary disease (COPD). In these diseases the overproduction or altered composition of MUC5AC contributes to airway obstruction and reduced pulmonary function. MUC5AC levels can be indicative of disease severity and are often measured via tests like the 5AC blood test. In these contexts proteins such as 5AC protein and 45M1 are often studied for their roles in facilitating disease progression and as potential therapeutic targets to modulate mucin levels in affected individuals.

Product promise

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [MUC5AC/917] (ab218466), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [MUC5AC/917] (ab218466)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling Mucin 5AC with ab218466 at 2 μg/ml.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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