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AB222845

Anti-Multicilin antibody

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(1 Publication)

Rabbit Polyclonal Multicilin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCIDAS aa 1-150.

View Alternative Names

IDAS, MCI, MCIN, MCIDAS, Multicilin, Multiciliate differentiation and DNA synthesis-associated cell cycle protein, Protein Idas, McIdas protein

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Multicilin antibody (AB222845)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Multicilin antibody (AB222845)

Paraffin embedded human prostate cancer tissue stained for Multicilin with ab222845 at a 1/100 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCIDAS aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

D6RGH6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Multicilin also known as multiciliate differentiation protein MCIDAS functions as a transcription co-activator that regulates the formation of multiciliated cells. With an approximate molecular weight of 34 kDa Multicilin mainly expresses in ciliated cell lineages especially within the respiratory and reproductive systems. This protein influences the expression of various genes necessary for the development of motile cilia playing a role in the differentiation process of epithelial cells into multiciliated cells.
Biological function summary

Multicilin plays a significant role by cooperating with the E2F4/5-DP1 transcription factor complex to induce multiciliogenesis. It promotes the transcription of genes essential for basal body formation and ciliogenesis essential steps in the development of motile cilia. When interacting with components like the Geminin coiled-coil domain-containing protein (GMNC) Multicilin triggers the transcriptional network that specifies the ciliated cell fate. Through this complex it contributes to generating multiple motile cilia on the surface of cells.

Pathways

Multicilin is integral to the Notch signaling pathway and the ciliogenesis pathway. In the Notch signaling context Multicilin functions by repressing Notch targets facilitating multiciliate cell differentiation. It works alongside proteins like E2F4/5 and brings about an upregulation of genes necessary for cilia. Multicilin as part of these pathways influences cellular processes that determine cell development and specialization through changes in the transcriptional landscape.

Multicilin's activity relates closely to primary ciliary dyskinesia (PCD) and respiratory conditions like chronic obstructive pulmonary disease (COPD). Dysregulation in Multicilin function affects cilia formation leading to PCD which results in impaired mucus clearance and chronic respiratory infections. The protein's connection to GMNC affects its role in PCD as disruptions in their interaction lead to ciliary defects. Understanding these interactions provides insights into therapeutic targets for respiratory disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription regulator specifically required for multiciliate cell differentiation (PubMed : 25048963). Acts in a multiprotein complex containing E2F4 and E2F5 that binds and activates genes required for centriole biogenesis. Required for the deuterosome-mediated acentriolar pathway (PubMed : 25048963). Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Modulates GMNN activity by reducing its affinity for CDT1 (PubMed : 21543332, PubMed : 24064211).
See full target information MCIDAS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Lung cellular and molecular physiology 324:L799-L814 PubMed37039368

2023

Age-associated differences in the human lung extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Maunick Lefin Koloko Ngassie,Maaike De Vries,Theo Borghuis,Wim Timens,Don D Sin,David Nickle,Philippe Joubert,Peter Horvatovich,György Marko-Varga,Jacob J Teske,Judith M Vonk,Reinoud Gosens,Y S Prakash,Janette K Burgess,Corry-Anke Brandsma
View all publications
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