Mouse Monoclonal NCAP antibody. Suitable for I-ELISA, WB and reacts with Mumps orthorubulavirus, Recombinant full length protein - Mumps orthorubulavirus samples. Cited in 13 publications.
Images
![Western blot - Anti-Mumps nucleoprotein antibody [7B10] (AB9880), expandable thumbnail](https://content.abcam.com/products/images/mumps-nucleoprotein-antibody-7b10-ab9880--western-blot-img129553.gif "Western blot - Anti-Mumps nucleoprotein antibody [7B10] (AB9880)")
Publications
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- The Journal of experimental medicine 219:2022Life-threatening viral disease in a novel form of autosomal recessive IFNAR2 deficiency in the Arctic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristopher J A Duncan,Morten K Skouboe,Sophie Howarth,Anne K Hollensen,Rui Chen,Malene L Børresen,Benjamin J Thompson,Jarmila Stremenova Spegarova,Catherine F Hatton,Frederik F Stæger,Mette K Andersen,John Whittaker,Søren R Paludan,Sofie E Jørgensen,Martin K Thomsen,Jacob G Mikkelsen,Carsten Heilmann,Daniela Buhas,Nina F Øbro,Jakob T Bay,Hanne V Marquart,M Teresa de la Morena,Joseph A Klejka,Matthew Hirschfeld,Line Borgwardt,Isabel Forss,Tania Masmas,Anja Poulsen,Francisco Noya,Guy Rouleau,Torben Hansen,Sirui Zhou,Anders Albrechtsen,Reza Alizadehfar,Eric J Allenspach,Sophie Hambleton,Trine H MogensenPubMed 35442417
- Frontiers in immunology 12:7960652021Rubella Virus Infected Macrophages and Neutrophils Define Patterns of Granulomatous Inflammation in Inborn and Acquired Errors of Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLudmila Perelygina,Raeesa Faisthalab,Emily Abernathy,Min-Hsin Chen,LiJuan Hao,Lionel Bercovitch,Diana K Bayer,Lenora M Noroski,Michael T Lam,Maria Pia Cicalese,Waleed Al-Herz,Arti Nanda,Joud Hajjar,Koen Vanden Driessche,Shari Schroven,Julie Leysen,Misha Rosenbach,Philipp Peters,Johannes Raedler,Michael H Albert,Roshini S Abraham,Hemalatha G Rangarjan,David Buchbinder,Lisa Kobrynski,Anne Pham-Huy,Julie Dhossche,Charlotte Cunningham Rundles,Anna K Meyer,Amy Theos,T Prescott Atkinson,Amy Musiek,Mehdi Adeli,Ute Derichs,Christoph Walz,Renate Krüger,Horst von Bernuth,Christoph Klein,Joseph Icenogle,Fabian Hauck,Kathleen E SullivanPubMed 35003119
- Frontiers in immunology 12:5804542021Characterization of an Antiviral Component in Human Seminal Plasma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRan Chen,Wenjing Zhang,Maolei Gong,Fei Wang,Han Wu,Weihua Liu,Yunxiao Gao,Baoxing Liu,Song Chen,Wei Lu,Xiaoqin Yu,Aijie Liu,Ruiqin Han,Yongmei Chen,Daishu HanPubMed 33679733
- Virologica Sinica 36:521-5362020Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoqiang Hao,Yilong Wang,Mengying Zhu,Dongming Zhou,Rongxian Liu,Bin Wang,Yao-Wei Huang,Zhengyan ZhaoPubMed 33284397
- Vaccines 8:2020Protective Malaria Vaccine in Mice Based on the Circumsporozoite Protein Fused with the Mumps Nucleocapsid Protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodolfo F Marques et. al.PubMed 32325874
- FASEB journal : official publication of the Federa 33:12528-125402019Mumps virus infection disrupts blood-testis barrier through the induction of TNF-α in Sertoli cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHan Wu et. al.PubMed 31450968
- IEEE journal of solid-state circuits 53:2054-20642018High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander C Sun et. al.PubMed 30559530
- Scientific reports 7:433952017GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael K Lo,Robert Jordan,Aaron Arvey,Jawahar Sudhamsu,Punya Shrivastava-Ranjan,Anne L Hotard,Mike Flint,Laura K McMullan,Dustin Siegel,Michael O Clarke,Richard L Mackman,Hon C Hui,Michel Perron,Adrian S Ray,Tomas Cihlar,Stuart T Nichol,Christina F SpiropoulouPubMed 28262699
- Vaccine 34:4166-41712016Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSigrid Gouma et. al.PubMed 27372154
- Journal of immunological methods 431:52-92016Development of an IFNγ ELISPOT for the analysis of the human T cell response against mumps virus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWanda G H Han et. al.PubMed 26872407
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| I-ELISA | WB | |
|---|---|---|
| Mumps orthorubulavirus | Expected | Predicted |
| Recombinant full length protein - Mumps orthorubulavirus | Not recommended | Tested |
I-ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mumps orthorubulavirus | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mumps orthorubulavirus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mumps orthorubulavirus | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mumps orthorubulavirus | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
NP, Nucleocapsid protein, Nucleoprotein
Recommended products
Mouse Monoclonal NCAP antibody. Suitable for I-ELISA, WB and reacts with Mumps orthorubulavirus, Recombinant full length protein - Mumps orthorubulavirus samples. Cited in 13 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 7B10
- Purification technique
- Affinity purification Protein A
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR293636-4 and GR137414-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mumps nucleoprotein sometimes referred to as N protein plays an important role in the replication and transcription of the mumps virus acting as a structural element in the formation of viral ribonucleoprotein (RNP) complexes. The nucleoprotein is a large protein with a molecular mass of approximately 60 kDa and is expressed in cells that are infected by the mumps virus. It facilitates encapsidation of viral RNA and ensures the stability of the viral genome which is essential for virus survival and propagation.
- Biological function summary
The mumps nucleoprotein functions as a part of the mumps virus replication complex. It interacts with other viral proteins such as the phosphoprotein (P protein) and the large protein (L protein) to initiate and regulate the replication of viral RNA. These interactions are critical for the stabilization of the entire complex ensuring the efficient production of new viral particles within host cells. The nucleoprotein is primarily located in the cytoplasm of infected cells where it associates with newly synthesized viral RNA strands.
- Pathways
The mumps nucleoprotein is involved in the viral replication and transcription pathways specific to the mumps virus. These pathways facilitate viral spread and infection contributing to the virulence of the virus. The nucleoprotein works closely with L and P proteins which serve vital roles in the RNA polymerase complex guiding the synthesis of viral RNA. These interactions are part of the broader negative-strand RNA virus replication pathway shared by other viruses in the Paramyxoviridae family.
- Associated diseases and disorders
The mumps nucleoprotein is directly associated with the mumps disease a contagious viral infection that can lead to severe complications such as encephalitis and orchitis. Antibodies targeting the nucleoprotein can help in diagnosing mumps infections. The nucleoprotein also has implications in mumps-related complications where it interacts with immune system components. Research into this protein helps in understanding the immunological response to the mumps virus potentially informing vaccine development and antiviral therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)
Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
Lane 1 - prestained standard proteins
Lane 2 - mumps NP
Antibody 7B10 at a concentration of 0.5 μg/ml.Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
Lane 1 - prestained standard proteins
Lane 2 - mumps NP
Antibody 7B10 at a concentration of 0.5 μg/ml.All lanes: Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)
Predicted band size: 66 kDa
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