AB109113

Anti-Munc 13-4 antibody [EPR4914]

RabMAb
Recombinant
20ul selling size
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Munc 13-4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

Protein unc-13 homolog D, Munc13-4, UNC13D

2 Images

Flow Cytometry (Intracellular) - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

Intracellular flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.

Unknown

Western blot - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

All lanes:

Western blot - Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Molt-4 cell lysate at 10 µg

Lane 5:

RAW264.7 cell lysate at 10 µg

Predicted band size: 123 kDa

false

Carrier free

Anti-Munc 13-4 antibody [EPR4914] - BSA and Azide free
660 APC

APC Anti-Munc 13-4 antibody [EPR4914]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Munc 13-4 antibody [EPR4914]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Munc 13-4 antibody [EPR4914]
578 PE

PE Anti-Munc 13-4 antibody [EPR4914]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4914

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc13-4 also known as UNC13D acts as a critical mediator in the fusion of vesicles particularly in the exocytosis of cytotoxic granules in immune cells. This protein weighs approximately 120 kDa and resides in various immune cell types such as cytotoxic T lymphocytes and natural killer cells. Munc13-4 exists in the cytosol and relocates to the cell membrane upon activation supporting its role in exocytosis.

Biological function summary

Munc13-4 serves an essential function in immune response by facilitating granule-mediated cytotoxicity. This protein does not operate alone; it acts within a complex network with other members of the Unc13 protein family. The synergy between these proteins ensures the precise docking and priming of secretory vesicles aiding in effective immune responses against infected or malignant cells.

Pathways

Munc13-4 participates in the granule exocytosis pathway a central component of immune system functionality. Within this pathway Munc13-4 acts alongside proteins such as Syntaxin 11 and Rab27a influencing the regulation and release of cytolytic granules. Another vital pathway involving Munc13-4 is the calcium-dependent signaling pathway which modulates its activity and highlights its exocytotic role.

Defects in Munc13-4 have a strong association with familial hemophagocytic lymphohistiocytosis type 3 (FHL3) a severe immunological disorder. The impaired function of Munc13-4 results in defective cytotoxic granule exocytosis leading to improper immune regulation. Additionally connections exist between Munc13-4 dysfunction and systemic lupus erythematosus (SLE) which implicates proteins such as Perforin and Rab27a in altered immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.

See full target information UNC13D

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed33093239

2020

Unc-13 homolog D mediates an antiviral effect of the chromosome 19 microRNA cluster miR-517a.

Applications

Unspecified application

Species

Unspecified reactive species

Kamil Krawczynski,Yingshi Ouyang,Jean-Francois Mouillet,Tianjiao Chu,Carolyn B Coyne,Yoel Sadovsky

Human gene therapy 31:626-638 PubMed32253931

2020

Lentiviral Gene Therapy for Familial Hemophagocytic Lymphohistiocytosis Type 3, Caused by Genetic Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah E Takushi,Na Yoon Paik,Andrew Fedanov,Chengyu Prince,Christopher B Doering,H Trent Spencer,Shanmuganathan Chandrakasan

Molecular medicine reports 18:1015-1024 PubMed29767240

2018

Munc13‑4 mediates human neutrophil elastase‑induced airway mucin5AC hypersecretion by interacting with syntaxin2.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xu,Jia Zhou,Xiang-Dong Zhou,Qi Li,Juliy M Perelman,Victor P Kolosov

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Munc 13-4 antibody [EPR4914]

Flow Cytometry (Intracellular) - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

Western blot - Anti-Munc 13-4 antibody [EPR4914] (AB109113)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise