Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free

18 product images

• 

  Western blot - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Negative control: liver (PMID: 24309898), testis (PMID: 24309898).

  Lysates were freshly made and used immediately to minimize protein degradation.

  The identity of the lower MW band at approximately 100 kDa is unknown.

  This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

  All lanes: Western blot - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate at 20 µg

  Lane 2: Rat liver tissue lysate at 20 µg

  Lane 3: Rat testis tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Developed using the ECL technique.

  Observed band size: 200 kDa

  Exposure time: 48s

• 

  Immunoprecipitation - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Munc13-1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Rat brain tissue lysate 10 ug
  Lane 2: ABAB307511 IP in Rat brain tissue lysate
  Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Munc13-1 antibody [EPR27022-47] ab307511 in rat brain tissue lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 41 seconds

  All lanes: Immunoprecipitation - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate 10 μg

  Lane 2: Rat brain tissue lysate

  Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 200 kDa

  Exposure time: 41s

• 

  Immunoprecipitation - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Munc13-1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse brain tissue lysate 10 ug
  Lane 2: ABAB307511 IP in Mouse brain tissue lysate
  Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Munc13-1 antibody [EPR27022-47] ab307511 in mouse brain tissue lysate
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  Exposure time: 10 seconds

  All lanes: Immunoprecipitation - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate 10 μg

  Lane 2: Mouse brain tissue lysate

  Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 200 kDa

  Exposure time: 10s

• 

  Western blot - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using 307511, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Negative control: adrenal gland (PMID: 26575293).
  
  In lanes 1-6, the lysates were stored at -80Ўж prior to Western Blotting. The bands beneath the target band (200 kDa) are likely to be degradation products. In lane 7, the lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.
  
  The identity of the lower MW band at approximately 100 kDa is unknown.
  
  Exposure time:

  All lanes: Western blot - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate 20 μg

  Lanes 2 and 7: Mouse hippocampus tissue lysate 20 μg

  Lane 3: Mouse adrenal gland tissue lysate 20 μg

  Lane 4: Rat brain tissue lysate 20 μg

  Lane 5: Rat hippocampus tissue lysate 20 μg

  Lane 6: Rat adrenal gland tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 200 kDa

  Exposure time: 26s

• 

  Western blot - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using 307511, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: Negative control: liver (PMID: 24309898 ), testis (PMID: 24309898), spleen (PMID: 24309898).
  
  Lysates were freshly made and used immediately to minimize protein degradation.
  
  The identity of the lower MW band at approximately 100 kDa is unknown.
  15 seconds
  Exposure time:

  All lanes: Western blot - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate 20 μg

  Lane 2: Mouse liver tissue lysate 20 μg

  Lane 3: Mouse testis tissue lysate 20 μg

  Lane 4: Mouse spleen tissue lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 200 kDa

  Exposure time: 15s

• 

  Western blot - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using 307511, the same antibody clone in a different buffer formulation.
  Blocking and diluting buffer and concentration: This antibody does not cross-react with mouse Unc13b.
  Lanes 1-3: 26 seconds, Lanes 4-6: 59 seconds, Lane 7: 15 seconds.
  Exposure time:

  All lanes: Western blot - Anti-Munc13-1 antibody [EPR27022-47] (Anti-Munc13-1 antibody [EPR27022-47] ab307511) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containi a myc-his tag whole cell lysate 20 μg

  Lane 2: 293T cells transfected with a mouse Unc13a expression vector containi a his tag whole cell lysate 20 μg

  Lane 3: 293T cells transfected with a mouse Unc13b expression vector containi a myc-his tag whole cell lysate 20 μg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 200 kDa

  Exposure time: 3s

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/4000 (0.134 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on rat liver. The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/4000 (0.134 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat hippocampus. The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/4000 (0.134 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on mouse liver. The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/4000 (0.134 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse hippocampus. The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat liver (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat hippocampus (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat adrenal gland (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on rat adrenal gland (PMID:?26575293). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse liver (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse hippocampus (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse adrenal gland (fresh) tissue lABeling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 (10.72 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Low expression: confocal image showing no staining on mouse adrenal gland (PMID:?26575293). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Munc13-1 antibody [EPR27022-47] ab307511 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

• 

  Flow Cytometry (Intracellular) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Rat primary neuron cells lABelling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

• 

  Flow Cytometry (Intracellular) - Anti-Munc13-1 antibody [EPR27022-47] - BSA and Azide free (ab307512)

  This data was developed using Anti-Munc13-1 antibody [EPR27022-47] ab307511, the same antibody clone in a different buffer formulation.
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized Mouse primary neuron cells lABelling Munc13-1 with Anti-Munc13-1 antibody [EPR27022-47] ab307511 at 1/50 dilution (1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.