AB3451

Anti-Munc18-1 antibody

Name: Anti-Munc18-1 antibody (ab3451) | Abcam
Brand: Abcam Limited
SKU: AB3451
Price: 585 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(13 Publications)

Rabbit Polyclonal Munc18-1 antibody. Suitable for ICC, WB and reacts with Human, Mouse, Rat samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human STXBP1 aa 50-100.

View Alternative Names

UNC18A, STXBP1, Syntaxin-binding protein 1, MUNC18-1, N-Sec1, Protein unc-18 homolog 1, Protein unc-18 homolog A, p67, Unc18-1, Unc-18A

5 Images

AbReview41009****

Western blot - Anti-Munc18-1 antibody (AB3451)

All lanes:

Western blot - Anti-Munc18-1 antibody (ab3451) at 1/5000 dilution

All lanes:

Mouse hippocampal homogenate at 5 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/unavailable/goat-rabbit-igg-hl-hrp-preadsorbed-ab97069'>ab97069</a>) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 67 kDa

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Exposure time: 1min

This image is courtesy of an anonymous Abreview

Supplier Data

Immunocytochemistry - Anti-Munc18-1 antibody (AB3451)

Immunocytochemistry analysis of 70% confluent log phase U-87 MG cells labeling Munc18-1 with ab3451 at 2μg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor^® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a : green). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Nuclei (Panel b : blue) were stained with SlowFade^® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor^® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supplier Data

Western blot - Anti-Munc18-1 antibody (AB3451)

All lanes:

Western blot - Anti-Munc18-1 antibody (ab3451) at 2 µg/mL

All lanes:

Mouse brain tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution

Predicted band size: 68 kDa

Observed band size: 67 kDa

false

Supplier Data

Western blot - Anti-Munc18-1 antibody (AB3451)

Detected by chemiluminescence

All lanes:

Western blot - Anti-Munc18-1 antibody (ab3451) at 2 µg/mL

Lane 1:

Mouse brain tissue lysate at 30 µg

Lane 2:

Rat brain tissue lysate at 30 µg

Lane 3:

Rat liver tissue lysate at 30 µg

Lane 4:

Rat kidney tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal™ at 1/4000 dilution

Predicted band size: 68 kDa

Observed band size: 67 kDa

false

Supplier Data

Western blot - Anti-Munc18-1 antibody (AB3451)

Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
1. Extract only.
2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.

All lanes:

Western blot - Anti-Munc18-1 antibody (ab3451)

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human STXBP1 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P61764

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract." }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract." }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract." }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: 99% PBS, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.

Biological function summary

This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.

Pathways

Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.

Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1 : 1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synaptic membranes (By similarity). May play a role in determining the specificity of intracellular fusion reactions.

See full target information STXBP1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Neurochemical research 48:791-803 PubMed36335177

2022

Munc18-1 Contributes to Hippocampal Injury in Septic Rats Through Regulation of Syntanxin1A and Synaptophysin and Glutamate Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Fajuan Tang,Lin Chen,Hu Gao,Yupeng Lei,Linli Pan,Dongqiong Xiao,Xihong Li

Molecular therapy : the journal of the American Society of Gene Therapy 30:2163-2175 PubMed35283272

2022

Base-edited cynomolgus monkeys mimic core symptoms of STXBP1 encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zongyang Lu,Siting He,Jian Jiang,Ling Zhuang,Yan Wang,Guang Yang,Xiaoyu Jiang,Yanhong Nie,Jiqiang Fu,Xiaotong Zhang,Yong Lu,Xinyan Bian,Hung-Chun Chang,Zhiqi Xiong,Xingxu Huang,Zhen Liu,Qiang Sun

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:7329-7339 PubMed34290081

2021

Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Chieh Wang,Christopher Weyrer,Diasynou Fioravante,Pascal S Kaeser,Wade G Regehr

Scientific reports 11:8656 PubMed33883618

2021

CAPS1 is involved in hippocampal synaptic plasticity and hippocampus-associated learning.

Applications

Unspecified application

Species

Unspecified reactive species

Chiaki Ishii,Natsumi Shibano,Mio Yamazaki,Tomoki Arima,Yuna Kato,Yuki Ishii,Yo Shinoda,Yugo Fukazawa,Tetsushi Sadakata,Yoshitake Sano,Teiichi Furuichi

Life (Basel, Switzerland) 10: PubMed32911644

2020

Extracellular Alpha-Synuclein Promotes a Neuroinhibitory Secretory Phenotype in Astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Bruno Di Marco Vieira,Rowan A W Radford,Junna Hayashi,Emma D Eaton,Ben Greenaway,Mark Jambas,Eugen B Petcu,Roger S Chung,Dean L Pountney

eLife 9: PubMed32073399

2020

haploinsufficiency impairs inhibition and mediates key neurological features of encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wu Chen,Zhao-Lin Cai,Eugene S Chao,Hongmei Chen,Colleen M Longley,Shuang Hao,Hsiao-Tuan Chao,Joo Hyun Kim,Jessica E Messier,Huda Y Zoghbi,Jianrong Tang,John W Swann,Mingshan Xue

Science signaling 12: PubMed30783011

2019

Disabling the Gβγ-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Zack Zurawski,Analisa D Thompson Gray,Lillian J Brady,Brian Page,Emily Church,Nicholas A Harris,Michael R Dohn,Yun Young Yim,Karren Hyde,Douglas P Mortlock,Carrie K Jones,Danny G Winder,Simon Alford,Heidi E Hamm

Journal of neurochemistry 138:525-31 PubMed27246255

2016

Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-β levels in brain.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin W Henderson,Erik G Gentry,Travis Rush,Juan C Troncoso,Madhav Thambisetty,Thomas J Montine,Jeremy H Herskowitz

PLoS genetics 8:e1003067 PubMed23209433

2012

CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function.

Applications

WB, IHC-FoFr

Species

Mouse, Mouse

Jacy L Wagnon,Michael Briese,Wenzhi Sun,Connie L Mahaffey,Tomaž Curk,Gregor Rot,Jernej Ule,Wayne N Frankel

Journal of neurochemistry 122:775-88 PubMed22694054

2012

Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome.

Applications

Unspecified application

Species

Unspecified reactive species

Malgorzata Bajor,Piotr Michaluk,Peter Gulyassy,Adrienna Katalin Kekesi,Gabor Juhasz,Leszek Kaczmarek

View all publications

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Product promise

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munc18-1-antibody-epr4850-ab124920

(1 reviews)

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