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AB183484

Anti-Munc18-1 (phospho S241) antibody

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(2 Publications)

Rabbit Polyclonal Munc18-1 phospho S241 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Stxbp1 phospho S241.

View Alternative Names

Syntaxin-binding protein 1, Protein unc-18 homolog 1, Protein unc-18 homolog A, Unc18-1, Unc-18A, Stxbp1

1 Images
Western blot - Anti-Munc18-1 (phospho S241) antibody (AB183484)
  • WB

Supplier Data

Western blot - Anti-Munc18-1 (phospho S241) antibody (AB183484)

All lanes:

Western blot - Anti-Munc18-1 (phospho S241) antibody (ab183484) at 1/1000 dilution

Lane 1:

Mouse hippocampus lysate

Lane 2:

Mouse hippocampus lysate treated with lambda phosphatase

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Mouse Stxbp1 phospho S241. The exact immunogen used to generate this antibody is proprietary information.

O08599

Specificity

ab183484 is specific for the ~65kDa Munc18-1 protein phosphorylated at Ser241. Immunolabeling is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab183484 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.24% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18-1 also known as Syntaxin-binding protein 1 is an important protein involved in the regulation of neurotransmitter release at synapses. It has a molecular mass of approximately 67 kDa. This protein is highly expressed in the brain where it plays a significant role in synaptic vesicle exocytosis. Munc18-1 interacts with components of the SNARE complex facilitating the docking and fusion of synaptic vesicles with the presynaptic membrane.
Biological function summary

This protein is important for the proper release of neurotransmitters into the synaptic cleft. Munc18-1 functions as a part of the neuronal exocytosis complex where it regulates the interaction with syntaxin-1 a core SNARE protein. This protein-protein interaction stabilizes an intermediate state necessary for vesicle priming and subsequent fusion ensuring efficient synaptic transmission. By controlling vesicle movement Munc18-1 influences communication between neurons.

Pathways

Munc18-1 integrates into the neurotransmitter release pathway which is central to synaptic function. It closely associates with the SNARE complex involving proteins such as SNAP-25 and synaptobrevin. Another important pathway is the synaptic vesicle cycle where Munc18-1 plays a role in vesicle docking and priming stages. Through these interactions it ensures that synaptic vesicles are ready and able to release their contents upon stimulation.

Alterations in Munc18-1 expression or function connect to neurological conditions like epilepsy and intellectual disability. In epilepsy dysregulation of neurotransmitter release due to modified Munc18-1 function disrupts synaptic communication leading to seizure activity. In the context of these disorders Munc18-1 interacts functionally with proteins such as VAMP2 contributing to the pathological state when disrupted. These interactions highlight the importance of Munc18-1 in maintaining normal neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins (By similarity). Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1 : 1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synaptic membranes (PubMed : 21445306, PubMed : 28821673). May play a role in determining the specificity of intracellular fusion reactions (By similarity).
See full target information Stxbp1 phospho S241
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Psychiatry investigation 22:10-25 PubMed39885788

2025

Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Cai,Seong Mi Lee,Yura Choi,Bomlee Lee,Soo Jung Im,Dong Hyeon Kim,Hyung Jun Choi,Jin Hee Kim,Yeni Kim,Boo Ahn Shin,Songhee Jeon

Bioengineered 13:2194-2206 PubMed35030977

2022

Enabled homolog (ENAH) regulated by RNA binding protein splicing factor 3b subunit 4 (SF3B4) exacerbates the proliferation, invasion and migration of hepatocellular carcinoma cells via Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoming Deng,Yufeng Luo,Yaoming Zhang,Jinfeng Zhang,Zongyun He
View all publications
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