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AB103976

Anti-Munc18-2 antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal Munc18-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STXBP2 aa 300-550.

View Alternative Names

UNC18B, STXBP2, Syntaxin-binding protein 2, Protein unc-18 homolog 2, Protein unc-18 homolog B, Unc18-2, Unc-18B

1 Images
Western blot - Anti-Munc18-2 antibody (AB103976)
  • WB

Unknown

Western blot - Anti-Munc18-2 antibody (AB103976)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-Munc18-2 antibody (ab103976) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HeLa whole cell lysate at 30 µg

Predicted band size: 100 kDa,30 kDa,33 kDa,35 kDa,37 kDa,39 kDa,45 kDa,51 kDa,57 kDa,62 kDa,63 kDa,66 kDa,67 kDa,71 kDa,74 kDa

Observed band size: 40 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human STXBP2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information.

Q15833

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  • 5
  • 52
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18-2 also known as Syntaxin-binding protein 2 is a 65 kDa protein that plays a role in vesicle transport machinery. It interacts with syntaxins to regulate the docking and fusion of vesicles with their target membranes. Munc18-2 is mainly expressed in hematopoietic cells where it facilitates regulated secretion.
Biological function summary

This protein facilitates the formation of secretory vesicle complexes. Munc18-2 is a part of the SNARE complex working synergistically with other components to ensure efficient vesicular transport and release. In immune cells this supports processes such as the exocytosis of lytic granules and cytokine release essential for immune response functions.

Pathways

Munc18-2 plays a role in the exocytosis and vesicular fusion pathways. It is closely associated with the SNARE proteins like VAMPs (vesicle-associated membrane proteins) and SNAPs (synaptosomal-associated proteins). In the immune system it is part of the machinery that mediates the transport and release pathways of granules and other vesicular contents.

Munc18-2 dysfunction is linked to familial hemophagocytic lymphohistiocytosis (FHL). This condition involves defective immune response due to hindered vesicular secretion leading to uncontrolled inflammation. Munc18-2 mutations affect the interaction with syntaxin 11 a SNARE protein which disturbs immune cell function contributing to the pathogenesis of this disorder.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells.
See full target information STXBP2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 14:295-310 PubMed35421597

2022

A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghong Li,Zhe Zhou,Yue Sun,Chang Sun,Karin Klappe,Sven C D van IJzendoorn

JCI insight 2: PubMed28724787

2017

Disrupted apical exocytosis of cargo vesicles causes enteropathy in FHL5 patients with Munc18-2 mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Georg F Vogel,Jorik M van Rijn,Iris M Krainer,Andreas R Janecke,Carsten Posovszky,Marta Cohen,Claire Searle,Prevost Jantchou,Johanna C Escher,Natalie Patey,Ernest Cutz,Thomas Müller,Sabine Middendorp,Michael W Hess,Lukas A Huber

The Journal of cell biology 211:587-604 PubMed26553929

2015

Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3.

Applications

Unspecified application

Species

Unspecified reactive species

Georg F Vogel,Katharina M C Klee,Andreas R Janecke,Thomas Müller,Michael W Hess,Lukas A Huber
View all publications
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Product promise

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