Rabbit Polyclonal Munc18-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STXBP2 aa 300-550.
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Publications
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- Cellular and molecular gastroenterology and hepatology 14:295-3102022A Functional Relationship Between UNC45A and MYO5B Connects Two Rare Diseases With Shared Enteropathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQinghong Li,Zhe Zhou,Yue Sun,Chang Sun,Karin Klappe,Sven C D van IJzendoornPubMed 35421597
- JCI insight 2:2017Disrupted apical exocytosis of cargo vesicles causes enteropathy in FHL5 patients with Munc18-2 mutations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorg F Vogel et. al.PubMed 28724787
- The Journal of cell biology 211:587-6042015Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGeorg F Vogel,Katharina M C Klee,Andreas R Janecke,Thomas Müller,Michael W Hess,Lukas A HuberPubMed 26553929
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human STXBP2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q15833
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Cow | Predicted |
| Dog | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog | Dilution info - | Notes - |
Target data
Function
Involved in intracellular vesicle trafficking and vesicle fusion with membranes. Contributes to the granule exocytosis machinery through interaction with soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins that regulate membrane fusion. Regulates cytotoxic granule exocytosis in natural killer (NK) cells.
Alternative names
UNC18B, STXBP2, Syntaxin-binding protein 2, Protein unc-18 homolog 2, Protein unc-18 homolog B, Unc18-2, Unc-18B
Recommended products
Rabbit Polyclonal Munc18-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human STXBP2 aa 300-550.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human STXBP2 aa 300-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q15833
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Munc18-2 also known as Syntaxin-binding protein 2 is a 65 kDa protein that plays a role in vesicle transport machinery. It interacts with syntaxins to regulate the docking and fusion of vesicles with their target membranes. Munc18-2 is mainly expressed in hematopoietic cells where it facilitates regulated secretion.
- Biological function summary
This protein facilitates the formation of secretory vesicle complexes. Munc18-2 is a part of the SNARE complex working synergistically with other components to ensure efficient vesicular transport and release. In immune cells this supports processes such as the exocytosis of lytic granules and cytokine release essential for immune response functions.
- Pathways
Munc18-2 plays a role in the exocytosis and vesicular fusion pathways. It is closely associated with the SNARE proteins like VAMPs (vesicle-associated membrane proteins) and SNAPs (synaptosomal-associated proteins). In the immune system it is part of the machinery that mediates the transport and release pathways of granules and other vesicular contents.
- Associated diseases and disorders
Munc18-2 dysfunction is linked to familial hemophagocytic lymphohistiocytosis (FHL). This condition involves defective immune response due to hindered vesicular secretion leading to uncontrolled inflammation. Munc18-2 mutations affect the interaction with syntaxin 11 a SNARE protein which disturbs immune cell function contributing to the pathogenesis of this disorder.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Munc18-2 antibody (ab103976)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-Munc18-2 antibody (ab103976) at 1/1000 dilution
Lane 1: H1299 whole cell lysate at 30 µg
Lane 2: HeLa whole cell lysate at 30 µg
Predicted band size: 100 kDa, 30 kDa, 33 kDa, 35 kDa, 37 kDa, 39 kDa, 45 kDa, 51 kDa, 57 kDa, 62 kDa, 63 kDa, 66 kDa, 67 kDa, 71 kDa, 74 kDa
Observed band size: 40 kDa
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