AB224625

Anti-Munc18c antibody

Name: Anti-Munc18c antibody (ab224625) | Abcam
Brand: Abcam Limited
SKU: AB224625

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(1 Publication)

Rabbit Polyclonal Munc18c antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Syntaxin-binding protein 3 aa 250-550.

View Alternative Names

Syntaxin-binding protein 3, Platelet Sec1 protein, Protein unc-18 homolog 3, Protein unc-18 homolog C, PSP, Unc18-3, Unc-18C, STXBP3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18c antibody (AB224625)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18c antibody (AB224625)

Paraffin-embedded human adrenal gland tissue stained for Munc18c using ab224625 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Munc18c antibody (AB224625)

All lanes:

Western blot - Anti-Munc18c antibody (ab224625) at 1/500 dilution

All lanes:

Mouse kidney tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Syntaxin-binding protein 3 aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

O00186

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18c also known as Syntaxin 1B or STXBP3 is a member of the Sec1/Munc18 family of proteins with a molecular mass of approximately 67 kDa. It is expressed in a variety of tissues but primarily appears in the pancreas adipose tissue and skeletal muscle. Mechanically Munc18c interacts with syntaxin proteins to facilitate the docking and fusion of vesicles playing a critical role in the transport of proteins and lipids across cellular membranes.

Biological function summary

The protein participates in cellular processes like membrane fusion and exocytosis and involves components of the SNARE complex. Munc18c supports the assembly of this complex by binding to syntaxin 4 a partner protein. By stabilizing syntaxin 4 Munc18c ensures the proper transmission of vesicles within cells impacting processes such as insulin-stimulated glucose uptake in muscle and fat cells.

Pathways

Munc18c plays an integral role in the insulin signaling pathway and is also significant in the GLUT4 trafficking pathway. In these pathways the protein associates with proteins such as synaptosome-associated protein 23 and Vesicle-associated membrane protein 2 (VAMP2). This involvement is essential for facilitating the movement of glucose transporter 4 (GLUT4) to the cell surface an important step in glucose homeostasis.

Defects in Munc18c function have been associated with type 2 diabetes and metabolic syndrome. Impaired Munc18c activity can lead to deficient insulin-stimulated glucose transport contributing to insulin resistance. The protein interacts with other molecules affected in these conditions such as Rab GTPase-activating proteins (RabGAPs) whose dysfunction further exacerbates metabolic irregularities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.

See full target information Syntaxin-binding protein 3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 61:16 PubMed32053730

2020

Electroretinographic Abnormalities and Sex Differences Detected with Mesopic Adaptation in a Mouse Model of Schizophrenia: A and B Wave Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalia Torres Jimenez,Justin W Lines,Rachel B Kueppers,Paulo Kofuji,Henry Wei,Amy Rankila,Joseph T Coyle,Robert F Miller,Linda K McLoon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Munc18c antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18c antibody (AB224625)

Western blot - Anti-Munc18c antibody (AB224625)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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