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Rabbit Polyclonal Munc18c antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human STXBP3 aa 250-550.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human STXBP3 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link O00186
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Expected
Tested
Mouse
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/500.00000 - 1/5000.00000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20.00000 - 1/200.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.

Alternative names

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Rabbit Polyclonal Munc18c antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human STXBP3 aa 250-550.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human STXBP3 aa 250-550. The exact immunogen used to generate this antibody is proprietary information. Database link O00186
Purification technique

Affinity purification Protein G

Concentration
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Purification notes

Purity >95%

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The protein participates in cellular processes like membrane fusion and exocytosis and involves components of the SNARE complex. Munc18c supports the assembly of this complex by binding to syntaxin 4 a partner protein. By stabilizing syntaxin 4 Munc18c ensures the proper transmission of vesicles within cells impacting processes such as insulin-stimulated glucose uptake in muscle and fat cells.

Activity summary

Munc18c also known as Syntaxin 1B or STXBP3 is a member of the Sec1/Munc18 family of proteins with a molecular mass of approximately 67 kDa. It is expressed in a variety of tissues but primarily appears in the pancreas adipose tissue and skeletal muscle. Mechanically Munc18c interacts with syntaxin proteins to facilitate the docking and fusion of vesicles playing a critical role in the transport of proteins and lipids across cellular membranes.

Pathways

Munc18c plays an integral role in the insulin signaling pathway and is also significant in the GLUT4 trafficking pathway. In these pathways the protein associates with proteins such as synaptosome-associated protein 23 and Vesicle-associated membrane protein 2 (VAMP2). This involvement is essential for facilitating the movement of glucose transporter 4 (GLUT4) to the cell surface an important step in glucose homeostasis.

Associated diseases and disorders

Defects in Munc18c function have been associated with type 2 diabetes and metabolic syndrome. Impaired Munc18c activity can lead to deficient insulin-stimulated glucose transport contributing to insulin resistance. The protein interacts with other molecules affected in these conditions such as Rab GTPase-activating proteins (RabGAPs) whose dysfunction further exacerbates metabolic irregularities.

Product promise

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2 product images

  • Western blot - Anti-Munc18c antibody (ab224625), expandable thumbnail

    Western blot - Anti-Munc18c antibody (ab224625)

    All lanes: Western blot - Anti-Munc18c antibody (AB224625) at 1/500 dilution

    All lanes: Mouse kidney tissue lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18c antibody (ab224625), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Munc18c antibody (ab224625)

    Paraffin-embedded human adrenal gland tissue stained for Munc18c using ab224625 at 1/100 dilution in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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