AB182004

Anti-Munc18c antibody [EPR12446]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Munc18c antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Syntaxin-binding protein 3, Platelet Sec1 protein, Protein unc-18 homolog 3, Protein unc-18 homolog C, PSP, Unc18-3, Unc-18C, STXBP3

3 Images

Flow Cytometry (Intracellular) - Anti-Munc18c antibody [EPR12446] (AB182004)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Munc18c antibody [EPR12446] (AB182004)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedK562 cells labeling Munc18cwith ab182004 at 1/300 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-Munc18c antibody [EPR12446] (AB182004)

All lanes:

Western blot - Anti-Munc18c antibody [EPR12446] (ab182004) at 1/1000 dilution

All lanes:

K562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Supplier Data

Western blot - Anti-Munc18c antibody [EPR12446] (AB182004)

All lanes:

Western blot - Anti-Munc18c antibody [EPR12446] (ab182004) at 1/5000 dilution

All lanes:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Carrier free

Anti-Munc18c antibody [EPR12446] - BSA and Azide free
660 APC

APC Anti-Munc18c antibody [EPR12446]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Munc18c antibody [EPR12446]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Munc18c antibody [EPR12446]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Munc18c antibody [EPR12446]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Munc18c antibody [EPR12446]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Munc18c antibody [EPR12446]
578 PE

PE Anti-Munc18c antibody [EPR12446]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12446

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18c also known as Syntaxin 1B or STXBP3 is a member of the Sec1/Munc18 family of proteins with a molecular mass of approximately 67 kDa. It is expressed in a variety of tissues but primarily appears in the pancreas adipose tissue and skeletal muscle. Mechanically Munc18c interacts with syntaxin proteins to facilitate the docking and fusion of vesicles playing a critical role in the transport of proteins and lipids across cellular membranes.

Biological function summary

The protein participates in cellular processes like membrane fusion and exocytosis and involves components of the SNARE complex. Munc18c supports the assembly of this complex by binding to syntaxin 4 a partner protein. By stabilizing syntaxin 4 Munc18c ensures the proper transmission of vesicles within cells impacting processes such as insulin-stimulated glucose uptake in muscle and fat cells.

Pathways

Munc18c plays an integral role in the insulin signaling pathway and is also significant in the GLUT4 trafficking pathway. In these pathways the protein associates with proteins such as synaptosome-associated protein 23 and Vesicle-associated membrane protein 2 (VAMP2). This involvement is essential for facilitating the movement of glucose transporter 4 (GLUT4) to the cell surface an important step in glucose homeostasis.

Defects in Munc18c function have been associated with type 2 diabetes and metabolic syndrome. Impaired Munc18c activity can lead to deficient insulin-stimulated glucose transport contributing to insulin resistance. The protein interacts with other molecules affected in these conditions such as Rab GTPase-activating proteins (RabGAPs) whose dysfunction further exacerbates metabolic irregularities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.

See full target information Syntaxin-binding protein 3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 135: PubMed34878135

2022

The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Tischer,Jing Yang,David Barford

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Munc18c antibody [EPR12446]

Flow Cytometry (Intracellular) - Anti-Munc18c antibody [EPR12446] (AB182004)

Western blot - Anti-Munc18c antibody [EPR12446] (AB182004)

Western blot - Anti-Munc18c antibody [EPR12446] (AB182004)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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