AB175238

Anti-Munc18c antibody [EPR12447]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Munc18c antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Syntaxin-binding protein 3, Platelet Sec1 protein, Protein unc-18 homolog 3, Protein unc-18 homolog C, PSP, Unc18-3, Unc-18C, STXBP3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (AB175238)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (AB175238)

Immunofluorescent analysis of K562 cells labeling Munc18c with ab175238 at 1/100 (red) and DAPI staining (blue).

Supplier Data

Western blot - Anti-Munc18c antibody [EPR12447] (AB175238)

All lanes:

Western blot - Anti-Munc18c antibody [EPR12447] (ab175238) at 1/10000 dilution

Lane 1:

Human fetal kidney tissue lysate at 10 µg

Lane 2:

Human spleen lysate at 10 µg

Lane 3:

K562 lysate at 10 µg

Predicted band size: 68 kDa

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Supplier Data

Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (AB175238)

Immunoprecipitation of Munc18c from Human fetal kidney lysate using ab175238 at 1/10

All lanes:

Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12447

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Munc18c also known as Syntaxin 1B or STXBP3 is a member of the Sec1/Munc18 family of proteins with a molecular mass of approximately 67 kDa. It is expressed in a variety of tissues but primarily appears in the pancreas adipose tissue and skeletal muscle. Mechanically Munc18c interacts with syntaxin proteins to facilitate the docking and fusion of vesicles playing a critical role in the transport of proteins and lipids across cellular membranes.

Biological function summary

The protein participates in cellular processes like membrane fusion and exocytosis and involves components of the SNARE complex. Munc18c supports the assembly of this complex by binding to syntaxin 4 a partner protein. By stabilizing syntaxin 4 Munc18c ensures the proper transmission of vesicles within cells impacting processes such as insulin-stimulated glucose uptake in muscle and fat cells.

Pathways

Munc18c plays an integral role in the insulin signaling pathway and is also significant in the GLUT4 trafficking pathway. In these pathways the protein associates with proteins such as synaptosome-associated protein 23 and Vesicle-associated membrane protein 2 (VAMP2). This involvement is essential for facilitating the movement of glucose transporter 4 (GLUT4) to the cell surface an important step in glucose homeostasis.

Defects in Munc18c function have been associated with type 2 diabetes and metabolic syndrome. Impaired Munc18c activity can lead to deficient insulin-stimulated glucose transport contributing to insulin resistance. The protein interacts with other molecules affected in these conditions such as Rab GTPase-activating proteins (RabGAPs) whose dysfunction further exacerbates metabolic irregularities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.

See full target information Syntaxin-binding protein 3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed38136609

2023

Phosphorylation of Syntaxin 4 by the Insulin Receptor Drives Exocytic SNARE Complex Formation to Deliver GLUT4 to the Cell Surface.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Kioumourtzoglou,Hannah L Black,Mohammed Al Tobi,Rachel Livingstone,John R Petrie,James G Boyle,Gwyn W Gould,Nia J Bryant

Molecular cancer research : MCR 20:434-445 PubMed34876482

2021

Syntaxin4-Munc18c Interaction Promotes Breast Tumor Invasion and Metastasis by Regulating MT1-MMP Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Megan I Brasher,Shawn C Chafe,Paul C McDonald,Oksana Nemirovsky,Genya Gorshtein,Zachary J Gerbec,Wells S Brown,Olivia R Grafinger,Matthew Marchment,Esther Matus,Shoukat Dedhar,Marc G Coppolino

The Journal of biological chemistry 292:16199-16210 PubMed28798239

2017

Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Megan I Brasher,David M Martynowicz,Olivia R Grafinger,Andrea Hucik,Emma Shanks-Skinner,James Uniacke,Marc G Coppolino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Munc18c antibody [EPR12447]

Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (AB175238)

Western blot - Anti-Munc18c antibody [EPR12447] (AB175238)

Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (AB175238)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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