Rabbit Recombinant Monoclonal MURF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 60 publications.
Images
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img110746.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)")
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img126387.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)")
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img64400.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)")
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img59423.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)")
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img91908.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)")
Publications
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- Biomolecules 13:2023Preventive Effects of Collagen-Derived Dipeptide Prolyl-Hydroxyproline against Dexamethasone-Induced Muscle Atrophy in Mouse C2C12 Skeletal Myotubes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshifumi Kimira,Konosuke Osawa,Yoshihiro Osawa,Hiroshi ManoPubMed 38002299
- Cancers 15:2023Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeili Tao,Ze Ouyang,Zhiqi Liao,Lu Li,Yujie Zhang,Jiali Gao,Li Ma,Shiying YuPubMed 37190306
- RMD open 9:2023Immunoproteasome subunit β5i promotes perifascicular muscle atrophy in dermatomyositis by upregulating RIG-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Zhang,Qisheng Xia,Wenli Li,Qingyan Liu,Lining Zhang,Xiaolan Tian,Lifang Ye,Guochun Wang,Qinglin PengPubMed 36854567
- Nutrients 15:2023The Inhibition of Autophagy and Pyroptosis by an Ethanol Extract of Leaf Contributes to the Amelioration of Dexamethasone-Induced Muscle Atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunji Park,Hojung Choi,Cao-Sang Truong,Hee-Sook JunPubMed 36839161
- Antioxidants (Basel, Switzerland) 12:2023Bavachin and Corylifol A Improve Muscle Atrophy by Enhancing Mitochondria Quality Control in Type 2 Diabetic Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyeong-Hoon Yeon,Eunhui Seo,Jong-Han Lee,Hee-Sook JunPubMed 36671000
- Nutrients 15:2022Quercetin Improved Muscle Mass and Mitochondrial Content in a Murine Model of Cancer and Chemotherapy-Induced Cachexia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrandon N VanderVeen,Thomas D Cardaci,Patrice Cunningham,Sierra J McDonald,Brooke M Bullard,Daping Fan,E Angela Murphy,Kandy T VelázquezPubMed 36615760
- Phytotherapy research : PTR 37:809-8192022Saikosaponin D alleviates cancer cachexia by directly inhibiting STAT3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin-Lin Chen,Liu-Yuan Xia,Jun-Ping Zhang,Yan Wang,Jian-Yu Chen,Cheng Guo,Wei-Heng XuPubMed 36447385
- Advances in rheumatology (London, England) 62:272022Vitamin D supplementation modulates autophagy in the pristane-induced lupus model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuela Dos Santos,Jordana Miranda de Souza Silva,Bárbara Jonson Bartikoski,Eduarda Correa Freitas,Amanda Busatto,Rafaela Cavalheiro do Espírito Santo,Odirlei Andre Monticielo,Ricardo Machado XavierPubMed 35869515
- Frontiers in oncology 12:8807872022Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMayra Tardelli de Jesus Testa,Paola Sanches Cella,Poliana Camila Marinello,Fernando Tadeu Trevisan Frajacomo,Camila de Souza Padilha,Patricia Chimin Perandini,Felipe Arruda Moura,José Alberto Duarte,Rubens Cecchini,Flavia Alessandra Guarnier,Rafael DeminicePubMed 35847939
- Frontiers in physiology 13:8975592022Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaoping Li,Guanguan Luo,Rong Zeng,Lian Lin,Xingnan Zou,Yu Yan,Haoli Ma,Jian Xia,Yan Zhao,Xianlong ZhouPubMed 35832486
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/5000 | Notes - |
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Function
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Additional Targets
TRIM55, TRIM54
Alternative names
IRF, MURF1, RNF28, SMRZ, TRIM63, E3 ubiquitin-protein ligase TRIM63, Iris RING finger protein, Muscle-specific RING finger protein 1, RING finger protein 28, RING-type E3 ubiquitin transferase TRIM63, Striated muscle RING zinc finger protein, Tripartite motif-containing protein 63, MuRF-1, MuRF1
Recommended products
Rabbit Recombinant Monoclonal MURF1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 60 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR6431(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img110746.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1: Human skeletal muscle lysate at 20 µg
Lane 2: Mouse muscle lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Observed band size: 40 kDa
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img126387.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1: Rat muscle tissue lysate at 10 µg
Lane 2: Mouse muscle tissue lysate at 10 µg
Lane 3: Human skeletal muscle tissue lysate at 10 µg
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img64400.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
All lanes: His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa
Exposure time: 30s
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img59423.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
All lanes: His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 45 kDa
Exposure time: 180s
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img91908.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution
All lanes: His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa
Exposure time: 150s
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img28624.jpg "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution
All lanes: Mouse muscle lysate at 20 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 133 kDa, 134 kDa, 28 kDa, 29 kDa, 34 kDa, 45 kDa, 51 kDa, 55 kDa, 58 kDa, 67 kDa, 90 kDa
Observed band size: 135 kDa, 40 kDa, 45 kDa, 50 kDa, 55 kDa, 58 kDa, 6 kDa, 67 kDa
Exposure time: 150s
![Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479), expandable thumbnail](https://content.abcam.com/products/images/murf1-murf3-murf2-antibody-epr64312-ab172479--western-blot-img28624.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)")
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