AB172479

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

RabMAb
Recombinant
What is this?

(1 Review)

(72 Publications)

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) is a rabbit monoclonal antibody detecting MURF1 in Western Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

View Alternative Names

IRF, MURF1, RNF28, SMRZ, TRIM63, E3 ubiquitin-protein ligase TRIM63, Iris RING finger protein, Muscle-specific RING finger protein 1, RING finger protein 28, RING-type E3 ubiquitin transferase TRIM63, Striated muscle RING zinc finger protein, Tripartite motif-containing protein 63, MuRF-1, MuRF1

6 Images

Lab

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Observed band size: 40 kDa

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Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

Lane 1:

Rat muscle tissue lysate at 10 µg

Lane 2:

Mouse muscle tissue lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate at 10 µg

false

Lab

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution

All lanes:

Mouse muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 133 kDa,134 kDa,28 kDa,29 kDa,34 kDa,45 kDa,51 kDa,55 kDa,58 kDa,67 kDa,90 kDa

Observed band size: 135 kDa,40 kDa,45 kDa,50 kDa,55 kDa,58 kDa,6 kDa,67 kDa

false

Exposure time: 150s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

All lanes:

His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

All lanes:

His-Tagged human MURF3 (full length) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 45 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution

All lanes:

His-Tagged human MURF2 (full length) recombinant protein at 1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Exposure time: 150s

Carrier free

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6431(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of MURF1?
Anti-MURF1 + MURF3 + MURF2 [EPR6431(2)] (ab172479) specifically detects a band for MURF1 (UniProt: Q969Q1) at a molecular weight of 40kDa.

Trusted by the scientific community
Anti-MURF1 + MURF3 + MURF2 [EPR6431(2)] (ab172479) was first used in a scientific publication in 2013 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

See full target information TRIM63

Additional targets

TRIM55,TRIM54

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Publications (72)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70542 PubMed40939109

2025

Combined effects of mechanical overload and high-intensity interval training on skeletal muscle hypertrophy in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hayato Shinkai,Takanaga Shirai,Kazuki Uemichi,Ryoto Iwai,Tomohiro Iwata,Riku Tanimura,Shunsuke Sugiyama,Tohru Takemasa

Food science & nutrition 13:e70624 PubMed40672545

2025

Extract of (Thunba.) Jussieu Fruit Reduces Muscle Atrophy in Cancer Cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Woo Woo,Pooreum Lim,Jihye Han,Ho Jin Kang,Jae Ho Shim,Jin Ju Lim,Seon-A Yoon,Hyejin Hyeon,Young-Min Ham,Ji-Gweon Park,Yong-Hwan Jung,Hyeon Soo Kim

Physiological reports 13:e70350 PubMed40323180

2025

Mild hyperbaric oxygen enhances recovery of the plantaris muscle atrophy induced by cast immobilization of the hindlimb in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ai Takemura,Tatsuro Egawa,Ryo Takagi,Ryota Iyama,Zhao Haiyu,Shinichiro Suzuki,Reika Fujino,Takuya Fukunaga,Tatsuya Hayashi,Satoshi Fujita

Cell biochemistry and biophysics 83:3069-3079 PubMed40089609

2025

Interaction of Programmed Obesity and Postnatal High Fat Diet but Not (-)-Epicatechin Treatment Modifies Muscle Atrophy Proteins Levels in Male Wistar Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Luisa Alvarez-Chávez,Sergio De Los Santos,Ramón Mauricio Coral-Vázquez,Luis Antonio Reyes-Castro,Elena Zambrano,Patricia Canto

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 133:e70014 PubMed40066528

2025

Faecalibacterium prausnitzii Suppresses Mitophagy to Alleviate Muscle Atrophy in Chronic Renal Failure With Protein-Energy Wasting.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Ni,Yuting Yin,Pan Liang,Yuanyuan Zheng,Yuying Li,Lvguang Pang,Xiaoshi Zhong,Jianguang Hu

Frontiers in physiology 15:1483594 PubMed39717825

2024

Entacapone alleviates muscle atrophy by modulating oxidative stress, proteolysis, and lipid aggregation in multiple mice models.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zeng,Hanbing Xu,Mingzheng Wu,Xianlong Zhou,Pan Lei,Jiangtao Yu,Pinyi Wang,Haoli Ma,Yan Zhao

Allergologia et immunopathologia 52:59-64 PubMed39278852

2024

Buzhong Yiqi decoction attenuates acquired myasthenia by regulating the JAK2/STAT3/AKT signaling pathway, inhibiting inflammation, and improving mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanfeng Zeng,Haihua Zeng,Xinghua Liu,Tingting Fan

Journal of cellular and molecular medicine 28:e18096 PubMed38149787

2023

Effects of resistance training on alleviating hypoxia-induced muscle atrophy: Focus on acetylation of FoxO1.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Fu,Rongxin Zhu,Weiyang Gao,Lijing Gong

Biomolecules 13: PubMed38002299

2023

Preventive Effects of Collagen-Derived Dipeptide Prolyl-Hydroxyproline against Dexamethasone-Induced Muscle Atrophy in Mouse C2C12 Skeletal Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshifumi Kimira,Konosuke Osawa,Yoshihiro Osawa,Hiroshi Mano

Cancers 15: PubMed37190306

2023

Ursolic Acid Alleviates Cancer Cachexia and Prevents Muscle Wasting via Activating SIRT1.

Applications

Unspecified application

Species

Unspecified reactive species

Weili Tao,Ze Ouyang,Zhiqi Liao,Lu Li,Yujie Zhang,Jiali Gao,Li Ma,Shiying Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (72)

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise