Anti-Mus81 antibody

Target data

Catalytic subunit of two functionally distinct, structure-specific, heterodimeric DNA endonucleases MUS81-EME1 and MUS81-EME2 that are involved in the maintenance of genome stability (PubMed : 11741546, PubMed : 12374758, PubMed : 12686547, PubMed : 12721304, PubMed : 24371268, PubMed : 24733841, PubMed : 24813886, PubMed : 35290797, PubMed : 39015284). Both endonucleases have essentially the same substrate specificity though MUS81-EME2 is more active than its MUS81-EME1 counterpart. Both cleave 3'-flaps and nicked Holliday junctions, and exhibit limited endonuclease activity with 5' flaps and nicked double-stranded DNAs (PubMed : 24371268, PubMed : 24733841, PubMed : 35290797). MUS81-EME2 which is active during the replication of DNA is more specifically involved in replication fork processing (PubMed : 24813886). Replication forks frequently encounter obstacles to their passage, including DNA base lesions, DNA interstrand cross-links, difficult-to-replicate sequences, transcription bubbles, or tightly bound proteins. One mechanism for the restart of a stalled replication fork involves nucleolytic cleavage mediated by the MUS81-EME2 endonuclease. By acting upon the stalled fork, MUS81-EME2 generates a DNA double-strand break (DSB) that can be repaired by homologous recombination, leading to the restoration of an active fork (PubMed : 24813886). MUS81-EME2 could also function in telomere maintenance (PubMed : 24813886). MUS81-EME1, on the other hand, is active later in the cell cycle and functions in the resolution of mitotic recombination intermediates including the Holliday junctions, the four-way DNA intermediates that form during homologous recombination (PubMed : 11741546, PubMed : 12374758, PubMed : 14617801, PubMed : 15805243, PubMed : 24813886).