AB14387

Anti-Mus81 antibody [MTA30 2G10/3]

Name: Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) | Abcam
Brand: Abcam Limited
SKU: AB14387
Price: 360 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(66 Publications)

Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) is a mouse monoclonal antibody detecting Mus81 in Western Blot, Flow Cytometry. Suitable for Human.

- Over 50 publications
- Trusted since 2004

View Alternative Names

Crossover junction endonuclease MUS81, MUS81

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Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (AB14387)

All lanes:

Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 61 kDa

Observed band size: 64 kDa

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Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (AB14387)

Flow Cyt

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Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (AB14387)

Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MTA30 2G10/3

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Native Full Length Protein corresponding to Human Crossover junction endonuclease MUS81.

Q96NY9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Tested on both recombinant protein and whole cell extract." }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Tested on both recombinant protein and whole cell extract." } } }

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Product details

What is this antibody validated in?
Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Human samples.

What is the molecular weight of Mus81?
Anti-Mus81 [MTA30 2G10/3] (ab14387) specifically detects a band for Mus81 (UniProt: Q96NY9) at a molecular weight of 66kDa.

Trusted by the scientific community
Anti-Mus81 [MTA30 2G10/3] (ab14387) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Mus81 [MTA30 2G10/3] (ab14387) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

from TCS

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Mus81 also known as methyl methanesulfonate 81 plays a significant role in DNA repair and genome stability. As a part of the XPF family of endonucleases Mus81 cuts DNA structures that arise during DNA replication and repair processes. It has a molecular mass of approximately 79 kDa and expresses in a range of organisms including humans. Mus81 is found in tissues with high cell turnover where DNA repair is necessary for maintaining genetic integrity.

Biological function summary

Mus81 functions as a component of the Mus81-Eme1 complex which facilitates the resolution of DNA junctions such as Holliday junctions stalled replication forks and other intermediates. This activity is important for the successful completion of homologous recombination and DNA repair safeguarding against mutations that may lead to genomic instability. Additionally Mus81 helps maintain the correct number of chromosomes during cell division by preventing or resolving DNA entanglement.

Pathways

Mus81 integrates into the pathway coordinating DNA damage response and repair. It is involved in the homologous recombination repair pathway essential for repairing double-strand breaks. Mus81 interacts with proteins such as Rad51 a pivotal protein in the homologous recombination process playing a collaborative role in restructuring damaged DNA. Mus81 provides flexibility and adaptability in orchestrating efficient DNA repair responses through these interactions.

Mus81 links to cancer progression and genetic disorders where DNA repair is compromised. For instance alterations in Mus81 function can lead to increased susceptibility to cancers due to the buildup of genomic instability. Additionally deficiencies in the Mus81 pathway can contribute to disorders such as Fanconi anemia which involves defective DNA repair. Mus81 also connects with proteins like BRCA2 another critical component in the DNA repair machinery highlighting its potential impact on cancer therapy strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Plays an essential role in mitosis for the processing of stalled or collapsed replication forks (PubMed : 28575661).

See full target information Crossover junction endonuclease MUS81

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Publications (66)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7214 PubMed40764480

2025

The RING finger E3 ligase RNF25 protects DNA replication forks independently of its canonical roles in ubiquitin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lilly F Chiou,Gaith N Droby,Deepika Jayaprakash,Jay R Anand,Xingyuan Zhang,Yang Yang,C Allie Mills,Thomas S Webb,Natalie K Barker,Jialiu Xie,Di Wu,Laura E Herring,Junya Tomida,Jessica L Bowser,Cyrus Vaziri

The EMBO journal 44:4525-4551 PubMed40624280

2025

TERRA R-loops trigger a switch in telomere maintenance towards break-induced replication and PRIMPOL-dependent repair.

Applications

Unspecified application

Species

Unspecified reactive species

Suna In,Patricia Renck Nunes,Rita Valador Fernandes,Joachim Lingner

Nature communications 16:1302 PubMed39900600

2025

RECQ4-MUS81 interaction contributes to telomere maintenance with implications to Rothmund-Thomson syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Raghib Ashraf,Hana Polasek-Sedlackova,Victoria Marini,Jana Prochazkova,Zdenka Hasanova,Magdalena Zacpalova,Michala Boudova,Lumir Krejci

Cell death discovery 11:31 PubMed39875375

2025

PARP inhibition-associated heterochromatin confers increased DNA replication stress and vulnerability to ATR inhibition in SMARCA4-deficient cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kimiyoshi Yano,Megumi Kato,Syoju Endo,Taichi Igarashi,Ryoga Wada,Takashi Kohno,Astrid Zimmermann,Heike Dahmen,Frank T Zenke,Bunsyo Shiotani

Molecular cell 84:2036-2052.e7 PubMed38688279

2024

Unprocessed genomic uracil as a source of DNA replication stress in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sneha Saxena,Christopher S Nabel,Turner W Seay,Parasvi S Patel,Ajinkya S Kawale,Caroline R Crosby,Helene Tigro,Eugene Oh,Matthew G Vander Heiden,Aaron N Hata,Zucai Suo,Lee Zou

Cell reports 43:114116 PubMed38625790

2024

RAD52-dependent mitotic DNA synthesis is required for genome stability in Cyclin E1-overexpressing cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia Audrey,Yannick P Kok,Shibo Yu,Lauren de Haan,Bert van de Kooij,Nathalie van den Tempel,Mengting Chen,H Rudolf de Boer,Bert van der Vegt,Marcel A T M van Vugt

Journal of molecular biology 435:168294 PubMed37777152

2023

Mitotic DNA Synthesis in Untransformed Human Cells Preserves Common Fragile Site Stability via a FANCD2-Driven Mechanism That Requires HELQ.

Applications

Unspecified application

Species

Unspecified reactive species

Emma L Traband,Sarah R Hammerlund,Mohammad Shameem,Ananya Narayan,Sanjiv Ramana,Anika Tella,Alexandra Sobeck,Naoko Shima

Cell reports 42:112792 PubMed37454295

2023

ATR protects ongoing and newly assembled DNA replication forks through distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Wendy Leung,Antoine Simoneau,Sneha Saxena,Jessica Jackson,Parasvi S Patel,Mangsi Limbu,Alessandro Vindigni,Lee Zou

BMC biology 21:110 PubMed37194054

2023

MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Marini,Fedor Nikulenkov,Pounami Samadder,Sissel Juul,Birgitta R Knudsen,Lumir Krejci

Nature communications 14:2428 PubMed37105990

2023

Pathway choice in the alternative telomere lengthening in neoplasia is dictated by replication fork processing mediated by EXD2's nuclease activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ronan Broderick,Veronica Cherdyntseva,Jadwiga Nieminuszczy,Eleni Dragona,Maria Kyriakaki,Theodora Evmorfopoulou,Sarantis Gagos,Wojciech Niedzwiedz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mus81 antibody [MTA30 2G10/3]

Western blot - Anti-Mus81 antibody [MTA30 2G10/3] (AB14387)

Flow Cytometry - Anti-Mus81 antibody [MTA30 2G10/3] (AB14387)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (66)

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