Anti-Musashi 1 / Msi1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB21628)

IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human small intestine* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB21628)

IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human hippocampus* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

Unknown

Western blot - Anti-Musashi 1 / Msi1 antibody (AB21628)

ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).

All lanes:

Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/mL

Lane 1:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (<a href='/en-us/products/unavailable/46c-mouse-neural-progenitor-selected-for-sox1-expression-cell-line-whole-cell-lysate-ab27205'>ab27205</a>) at 10 µg

Lane 3:

E14 Mouse Embryo Brain Tissue Lysate at 10 µg

Lane 4:

E10 Mouse Embryo Brain Tissue Lysate at 10 µg

Lane 5:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (<a href='/en-us/products/unavailable/human-musashi-1-msi1-peptide-ab23870'>ab23870</a>)

Lane 6:

46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (<a href='/en-us/products/unavailable/46c-mouse-neural-progenitor-selected-for-sox1-expression-cell-line-whole-cell-lysate-ab27205'>ab27205</a>) at 10 µg with Human Musashi 1 / Msi1 peptide (<a href='/en-us/products/unavailable/human-musashi-1-msi1-peptide-ab23870'>ab23870</a>)

Lane 7:

E14 Mouse Embryo Brain Tissue Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (<a href='/en-us/products/unavailable/human-musashi-1-msi1-peptide-ab23870'>ab23870</a>)

Lane 8:

E10 Mouse Embryo Brain Tissue Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (<a href='/en-us/products/unavailable/human-musashi-1-msi1-peptide-ab23870'>ab23870</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 39 kDa

Observed band size: 34 kDa,39 kDa

false