Rabbit Polyclonal Musashi 1 / Msi1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 34 publications.
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- Biological procedures online 25:262023Comparison of two protocols for the generation of iPSC-derived human astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrycja Mulica,Carmen Venegas,Zied Landoulsi,Katja Badanjak,Sylvie Delcambre,Maria Tziortziou,Soraya Hezzaz,Jenny Ghelfi,Semra Smajic,Jens Schwamborn,Rejko Krüger,Paul Antony,Patrick May,Enrico Glaab,Anne Grünewald,Sandro L PereiraPubMed 37730545
- Scientific reports 12:125132022Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoão Brás,Daniel Henriques,Ricardo Moreira,Magda M Santana,Rita Silva-Pedrosa,Diana Adão,Sandra Braz,Ana Rita Álvaro,Luís Pereira de Almeida,Liliana S MendonçaPubMed 35869235
- Experimental biology and medicine (Maywood, N.J.) 247:868-8792022Inhibition of Musashi-1 enhances chemotherapeutic sensitivity in gastric cancer patient-derived xenografts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Liu,Huan Yang,Xinyu Zhang,Xianglin Sun,Jiamin Zhou,Yuan Li,Yifei Liu,Zhixiang Zhuang,Guohua WangPubMed 35135374
- Environmental pollution (Barking, Essex : 1987) 285:1176542021The pyriproxyfen metabolite, 4'-OH-PPF, disrupts thyroid hormone signaling in neural stem cells, modifying neurodevelopmental genes affected by ZIKA virus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPieter Vancamp,Petra Spirhanzlova,Anthony Sébillot,Lucile Butruille,Jean-David Gothié,Sébastien Le Mével,Michelle Leemans,Karn Wejaphikul,Marcel Meima,Bilal B Mughal,Pierre Roques,Sylvie Remaud,Jean-Baptiste Fini,Barbara A DemeneixPubMed 34289950
- Frontiers in cellular and infection microbiology 11:5248512021The Effects of Fecal Microbiota Transplantation on the Symptoms and the Duodenal Neurogenin 3, Musashi 1, and Enteroendocrine Cells in Patients With Diarrhea-Predominant Irritable Bowel Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTarek Mazzawi,Magdy El-Salhy,Gülen Arslan Lied,Trygve HauskenPubMed 34055657
- Oncogenesis 10:292021Musashi-2 (MSI2) regulates epidermal growth factor receptor (EGFR) expression and response to EGFR inhibitors in EGFR-mutated non-small cell lung cancer (NSCLC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesPetr Makhov,Igor Bychkov,Bulat Faezov,Alexander Deneka,Alexander Kudinov,Emmanuelle Nicolas,Rohan Brebion,Eleanor Avril,Kathy Q Cai,Leonid V Kharin,Mark Voloshin,Elena Frantsiyants,Nikolay Karnaukhov,Oleg I Kit,Iuliia Topchu,Rushaniya Fazliyeva,Anna S Nikonova,Ilya G Serebriiskii,Hossein Borghaei,Martin Edelman,Essel Dulaimi,Erica A Golemis,Yanis BoumberPubMed 33723247
- Stem cell research & therapy 12:1692021Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiaoying Jia,Yan Cui,Zhigang Tan,Min Liu,Yugang JiangPubMed 33691791
- Endocrinology 162:2020Control of the Anterior Pituitary Cell Lineage Regulator POU1F1 by the Stem Cell Determinant Musashi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelody Allensworth-James,Jewel Banik,Angela Odle,Linda Hardy,Alex Lagasse,Ana Rita Silva Moreira,Jordan Bird,Christian L Thomas,Nathan Avaritt,Michael G Kharas,Christopher J Lengner,Stephanie D Byrum,Melanie C MacNicol,Gwen V Childs,Angus M MacNicolPubMed 33373440
- Cancer management and research 12:11257-112692020Down-Regulation of Circ_0032833 Sensitizes Colorectal Cancer to 5-Fluorouracil and Oxaliplatin Partly Depending on the Regulation of miR-125-5p and MSI1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShouchao Li et. al.PubMed 33177876
- Cells 9:2020Using High-Content Screening to Generate Single-Cell Gene-Corrected Patient-Derived iPS Clones Reveals Excess Alpha-Synuclein with Familial Parkinson's Disease Point Mutation A30P.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeter Barbuti et. al.PubMed 32927687
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC/IF | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Expected |
| Rat | Not recommended | Expected | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Alternative names
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Recommended products
Rabbit Polyclonal Musashi 1 / Msi1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 34 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Several customers have found that this antibody gives good results in rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
- Biological function summary
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
- Pathways
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
- Associated diseases and disorders
Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (ab21628)
IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human hippocampus* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (ab21628)
IHC image of Musashi 1 / Msi1 staining in a section of formalin-fixed paraffin-embedded normal human small intestine* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab21628, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628)
ab21628 recognized a band corresponding to the expected size of Musashi1/Msi1. The band was blocked using the immunising peptide (ab23870).
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody (ab21628) at 1 µg/mL
Lane 1: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 2: 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg
Lane 3: E14 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 4: E10 Mouse Embryo Brain Tissue Lysate at 10 µg
Lane 5: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (ab23870)
Lane 6: 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg with Human Musashi 1 / Msi1 peptide (ab23870)
Lane 7: E14 Mouse Embryo Brain Tissue Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (ab23870)
Lane 8: E10 Mouse Embryo Brain Tissue Lysate at 10 µg with Human Musashi 1 / Msi1 peptide (ab23870)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Predicted band size: 39 kDa
Observed band size: 34 kDa, 39 kDa
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