AB218189

Anti-Musashi 1 / Msi1 antibody

Name: Anti-Musashi 1 / Msi1 antibody (ab218189) | Abcam
Brand: Abcam Limited
SKU: AB218189

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(1 Publication)

Rabbit Polyclonal Musashi 1 / Msi1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MSI1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB218189)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB218189)

Formalin-fixed, paraffin-embedded mouse brain tissue stained for Musashi 1 / Msi1 using ab218189 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB218189)

Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for Musashi 1 / Msi1 using ab218189 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human MSI1 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43347

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.

Biological function summary

In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.

Pathways

The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.

Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

See full target information MSI1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 13:1112104 PubMed37124502

2023

Clustering analysis and prognostic model based on PI3K/AKT-related genes in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Deng,Xu He,Zehua Yang,Jing Huang,Lin Zhao,Min Wen,Xiyuan Hu,Zizheng Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Musashi 1 / Msi1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB218189)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody (AB218189)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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