Name: Anti-Musashi 1 / Msi1 antibody [EP1302]
SKU: ab52865
Rating: 4 (18 reviews)

Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit monoclonal antibody that is used to detect Musashi 1 / Msi1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Chicken, Human, Mouse, Quail samples.

- Specificity confirmed with Musashi 1 / Msi1 knockout cell line validation

Related conjugates and formulations

ab210418
Conjugated
PE Anti-Musashi 1 / Msi1 antibody [EP1302]
ab199781
Conjugated
Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302]
ab199838
Conjugated
Alexa Fluor® 647 Anti-Musashi 1 / Msi1 antibody [EP1302]
ab313185
Conjugated
Alexa Fluor® 555 Anti-Musashi 1 / Msi1 antibody [EP1302]
ab311702
Conjugated
Alexa Fluor® 594 Anti-Musashi 1 / Msi1 antibody [EP1302]
ab310871
Conjugated
APC Anti-Musashi 1 / Msi1 antibody [EP1302]
ab312977
Conjugated
Alexa Fluor® 568 Anti-Musashi 1 / Msi1 antibody [EP1302]
ab221797
Carrier free
Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free
ab321668
Conjugated
Alexa Fluor® 750 Anti-Musashi 1 / Msi1 antibody [EP1302]

Images

Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (AB52865), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Expected	Not recommended	Expected	Tested	Expected
Chicken	Expected	Not recommended	Tested	Expected	Expected
Quail	Tested	Not recommended	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Quail	Dilution info 1/50 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/50 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Chicken, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Quail, Chicken, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Chicken	Dilution info 1/1000	Notes For unpurified use at 1/2000.
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/2000.

Expected
Expected

Species	Dilution info	Notes
Species Quail, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250 - 1/500	Notes -
Species Human	Dilution info 1/250 - 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Quail, Chicken	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/80	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/40.

Expected
Expected

Species	Dilution info	Notes
Species Quail, Chicken, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

See full target information MSI1

Function

RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).

Alternative names

RNA-binding protein Musashi homolog 1, Musashi-1, MSI1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1302
Purification technique: Affinity purification Protein A
Specificity: Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

What is this antibody validated in?

Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Chicken, Human, Mouse, Quail samples.

What is the molecular weight of Musashi 1 / Msi1?

Anti-Musashi 1 / Msi1 [EP1302] (ab52865) specifically detects a band for Musashi 1 / Msi1 (UniProt: O43347) at a molecular weight of 39kDa.

Recommended positive controls

IHC-P: Human lung carcinoma tissue.Flow Cyt (intra): SH-SY5Y cells.ICC/IF: SH-SY5Y, Neuro-2a and HAP1-MSI1 cells.WB: SH-SY5Y cell lysate.

Trusted by the scientific community

Anti-Musashi 1 / Msi1 [EP1302] (ab52865) was first used in a scientific publication in 2007 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists

Anti-Musashi 1 / Msi1 [EP1302] (ab52865) has over 15 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed

The specificity of Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) has been confirmed by Western blot testing in MSI1 Knockout HAP1 cells.

Other related products

We have a range of other formats of antibody clone [EP1302] also available for your convenience:
ab52865, Alexa Fluor® 488 - ab199781, Alexa Fluor® 647 - ab199838, PE - ab210418, Carrier free - ab221797, APC - ab310871, Alexa Fluor® 594 - ab311702, Alexa Fluor® 568 - ab312977, Alexa Fluor® 555 - ab313185, Alexa Fluor® 750 - ab321668

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.

Biological function summary

In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.

Pathways

The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.

Associated diseases and disorders

Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
ab52865 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab52865 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Lanes 1 - 3: Merged signal (red and green). Green - ab52865 observed at 39 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. ab52865 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution
Lane 1: Wild-type HAP1 whole cell lysate
Lane 2: MSI1 knockout HAP1 whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Predicted band size: 39 kDa
Observed band size: 39 kDa
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 2: UMNSAH/DF-1 (chicken embryo fibroblast) whole cell lysates at 20 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1/80 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1:200. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution
All lanes: SH-SY-5Y cell lysate at 10 µg/mL
Secondary
All lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
This image is courtesy of a customer review submitted by Carl Hobbs.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
This image is courtesy of a customer review submitted by Jennifer Moore.
Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
Intracellular Intracellular Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC (Neural stem cells derived from human embryonic). The cells were fixed and permeabilized . The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.