Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit monoclonal antibody that is used to detect Musashi 1 / Msi1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Chicken, Human, Mouse, Quail samples.
- Specificity confirmed with Musashi 1 / Msi1 knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Tested | Tested |
Mouse | Expected | Not recommended | Expected | Tested | Expected |
Chicken | Expected | Not recommended | Tested | Expected | Expected |
Quail | Tested | Not recommended | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Quail | Dilution info 1/50 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/50 - 1/500 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Quail, Chicken, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken | Dilution info 1/1000 | Notes For unpurified use at 1/2000. |
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/2000. |
Species | Dilution info | Notes |
---|---|---|
Species Quail, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 - 1/500 | Notes - |
Species Human | Dilution info 1/250 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Quail, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/80 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/40. |
Species | Dilution info | Notes |
---|---|---|
Species Quail, Chicken, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) is a rabbit monoclonal antibody that is used to detect Musashi 1 / Msi1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Chicken, Human, Mouse, Quail samples.
- Specificity confirmed with Musashi 1 / Msi1 knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab52865 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab52865 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Lanes 1 - 3: Merged signal (red and green). Green - ab52865 observed at 39 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. ab52865 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution
Lane 1: Wild-type HAP1 whole cell lysate
Lane 2: MSI1 knockout HAP1 whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Predicted band size: 39 kDa
Observed band size: 39 kDa
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 2: UMNSAH/DF-1 (chicken embryo fibroblast) whole cell lysates at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1/80 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution
All lanes: SH-SY-5Y cell lysate at 10 µg/mL
All lanes: goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Intracellular Intracellular Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC (Neural stem cells derived from human embryonic). The cells were fixed and permeabilized . The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.
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