Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Quail, Human, Chicken, Mouse samples. Cited in 11 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img180462.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797)")
![Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--western-blot-img139969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111534.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797)")
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--flow-cytometry-intracellular-img2771.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797)")
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img14174.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (AB221797)")
Publications
Filter by
- Proceedings of the National Academy of Sciences of 113:6955-602016Musashi-2 (MSI2) supports TGF-β signaling and inhibits claudins to promote non-small cell lung cancer (NSCLC) metastasis.Applications:WBReactive species:Unspecified reactive speciesAlexander E Kudinov et. al.PubMed 27274057
- The Journal of endocrinology 226:135-432015Enhanced intestinal epithelial cell proliferation in diabetic rats correlates with β-catenin accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatiana Dorfman,Yulia Pollak,Rima Sohotnik,Arnold G Coran,Jacob Bejar,Igor SukhotnikPubMed 26297291
- PloS one 9:e869902014Multiple tumor suppressor microRNAs regulate telomerase and TCF7, an important transcriptional regulator of the Wnt pathway.Applications:WBReactive species:Unspecified reactive speciesRadmila Hrdličková et. al.PubMed 24551047
- World journal of gastroenterology 19:6637-442013Expression and significance of Musashi-1 in gastric cancer and precancerous lesions.Applications:IHC-PReactive species:HumanRong-Guang Kuang,Yan Kuang,Qing-Feng Luo,Cheng-Jun Zhou,Rui Ji,Jian-Wen WangPubMed 24151393
- BMC cancer 13:482013Expression of LGR-5, MSI-1 and DCAMKL-1, putative stem cell markers, in the early phases of 1,2-dimethylhydrazine-induced rat colon carcinogenesis: correlation with nuclear β-catenin.
- In vivo (Athens, Greece) 26:693-7022012Metallothionein colon crypt immuno-positivity as a rapid in vivo essay for drug efficacy studies.Applications:IHC-PReactive species:MouseFederica Mori,Carlo Della Rocca,Domenico LazzaroPubMed 22773584
- Translational oncology 5:141-542012A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles.
- Molecular cancer research : MCR 10:143-552012The oncogenic RNA-binding protein Musashi1 is regulated by HuR via mRNA translation and stability in glioblastoma cells.Applications:WBReactive species:HumanDat T Vo,Kotb Abdelmohsen,Jennifer L Martindale,Mei Qiao,Kumiko Tominaga,Tarea L Burton,Jonathan A L Gelfond,Andrew J Brenner,Vyomesh Patel,Daniel Trageser,Björn Scheffler,Myriam Gorospe,Luiz O F PenalvaPubMed 22258704
- Journal of clinical pathology 64:771-52011Lymphoid aggregates may contribute to the migration and epithelial commitment of bone marrow-derived cells in colonic mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGábor Valcz et. al.PubMed 21653659
- Stem cells (Dayton, Ohio) 29:154-612011Epithelial-connective tissue interactions induced by thyroid hormone receptor are essential for adult stem cell development in the Xenopus laevis intestine.Applications:IHC-PReactive species:Xenopus laevisTakashi Hasebe,Daniel R Buchholz,Yun-Bo Shi,Atsuko Ishizuya-OkaPubMed 21280164
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested | Tested |
| Mouse | Expected | Not recommended | Expected | Tested | Expected |
| Chicken | Expected | Not recommended | Tested | Expected | Expected |
| Quail | Tested | Not recommended | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Quail | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Quail, Chicken, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Quail, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Quail, Chicken | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Quail, Chicken, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
6 products for Alternative Version
Target data
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Alternative names
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Recommended products
Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Quail, Human, Chicken, Mouse samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP1302
- Purification technique
- Affinity purification Protein A
- Specificity
Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221797 is the carrier-free version of Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
- Biological function summary
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
- Pathways
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
- Associated diseases and disorders
Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img180462.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865)
Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 at 1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
![Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--western-blot-img139969.jpg "Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Lanes 1 - 3: Merged signal (red and green). Green - Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 observed at 39 kDa. Red - loading control, Anti-Vinculin antibody [VIN-54] ab130007, observed at 130 kDa.
Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 and Anti-Vinculin antibody [VIN-54] ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797) at 1/2000 dilution
Lane 1: Wild-type HAP1 whole cell lysate
Lane 2: MSI1 knockout HAP1 whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111534.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--flow-cytometry-intracellular-img2771.jpg "Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 at 1/80 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img14174.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img14893.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90038.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
This image is courtesy of a customer review submitted by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--flow-cytometry-intracellular-img137431.jpg "Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
This image is courtesy of a customer review submitted by Jennifer Moore.Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
Intracellular Intracellular Flow Cyt image of Musashi1 (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865)using Accutase digested single cell suspension of hESC. the cells were fixed adn permeabilized . The cells were incubated with unpurified Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Musashi 1 / Msi1 antibody [EP1302] ab52865).
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunocytochemistry-immunofluorescence-img14893.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img90038.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-ep1302-bsa-and-azide-free-ab221797--flow-cytometry-intracellular-img137431.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com