Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403486.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171)")
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403495.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171)")
![Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunoprecipitation-img403492.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171)")
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403496.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171)")
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--flow-cytometry-intracellular-img403485.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (AB305171)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Expected |
| Mouse | Tested | Tested | Not recommended | Tested | Tested |
| Rat | Tested | Tested | Not recommended | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes This antibody is not suitable for human species in IHC-P application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Alternative names
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Recommended products
Rabbit Recombinant Monoclonal Musashi 1 / Msi1 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26106-92
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human IHC-P.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
- Biological function summary
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
- Pathways
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
- Associated diseases and disorders
Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403486.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on mouse cerebellum (PMID: 11588182). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.Positive staining on mouse cerebellum (PMID: 11588182). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403495.jpg "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression tissues: kidney, heart (PMID: 8660864).
Exposure time: 3 minutesAll lanes: Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) at 1/1000 dilution
Lane 1: Mouse P0 brainstem tissue lysate at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Mouse heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunoprecipitation-img403492.jpg "Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Musashi 1 / Msi1 was immunoprecipitated from 0.35 mg Mouse P0 brainstem tissue lysate 10 ug with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse P0 brainstem tissue lysate 10 ug
Lane 2: Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 IP in Mouse P0 brainstem tissue lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 in mouse P0 brainstem tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 84 secondsAll lanes: Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) at 1/1000 dilution
Lane 1: Mouse P0 brainstem tissue lysate 10 ug
Lane 2: Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 IP in Mouse P0 brainstem tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 84s
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403496.jpg "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lysates at 20 µg per lane.
Performed under reducing conditions.
False colour image of Western blot: Anti-Musashi 1 / Msi1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 was shown to bind specifically to Musashi 1 / Msi1. A band was observed at 39 kDa in wild-type HAP1 cell lysates with no signal observed at this size in Musashi 1 / Msi1 knockout cell line (knockout cell lysate). To generate this image, wild-type and Musashi 1 / Msi1 knockout HAP1 cell lysates were analyzed. First, samples were run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in in Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: Musashi 1 / Msi1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
SecondaryLanes 1 - 4: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Lanes 1 - 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Flow Cytometry (Intracellular) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--flow-cytometry-intracellular-img403485.jpg "Flow Cytometry (Intracellular) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Flow Cytometry (Intracellular) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast, Left) / Neuro-2a (mouse neuroblastoma neuroblast, Right) cells labelling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: NIH/3T3 (PMID: 11359897).![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403493.jpg "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Both recombinant proteins were made in-house and expressed from the E. coli expression systems.
This antibody does not cross-react with mouse Musashi 2 / Msi2.
Exposure time: 8 secondsAll lanes: Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) at 1/1000 dilution
Lane 1: His-tagged mouse Musashi 1 / Msi1 recombinant protein (aa52-251) at 10 ng
Lane 2: His-tagged mouse Musashi 2 / Msi2 recombinant protein (aa1-346) at 10 ng
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 8s
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403494.jpg "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: NIH/3T3 (PMID:11359897).
Exposure time: 59 secondsAll lanes: Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170) at 1/1000 dilution
Lane 1: Mouse embryonic brain tissue lysate at 20 µg
Lane 2: Rat embryonic brain tissue lysate at 20 µg
Lane 3: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 59s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403487.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Negative control: No staining on mouse kidney (PMID: 25717188). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403488.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on intestinal crypts of mouse (PMID: 12558601). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403489.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on mouse testis (PMID: 11804968). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403490.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on rat cerebellum (PMID: 11588182). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403491.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)
This data was developed using Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat small intestine tissue labeling Musashi 1 / Msi1 with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 at 1/500 (0.918 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection). Positive staining on intestinal crypts of rat (PMID: 12558601). The section was incubated with Anti-Musashi 1 / MSI1 antibody [EPR26106-92] ab305170 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403493.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--western-blot-img403494.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403487.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403488.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403489.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403490.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-epr26106-92-bsa-and-azide-free-ab305171--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img403491.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] - BSA and Azide free (ab305171)")
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