Mouse Monoclonal Musashi 1 / Msi1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--immunocytochemistry-immunofluorescence-img168538.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819)")
![Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--western-blot-img104840.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819)")
![Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--western-blot-img87652.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (AB129819)")
Publications
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- International journal of oncology 61:2022Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Qian,You Wang,Yunxiang Ji,Danmin Chen,Chuanfang Wang,Guilong Zhang,Yezhong WangPubMed 35929514
- Neuron 67:407-212010Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.Applications:IPReactive species:Unspecified reactive speciesKen-ichiro Kuwako,Kyoko Kakumoto,Takao Imai,Mana Igarashi,Takao Hamakubo,Shin-ichi Sakakibara,Marc Tessier-Lavigne,Hirotaka James Okano,Hideyuki OkanoPubMed 20696379
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
Alternative names
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1
Recommended products
Mouse Monoclonal Musashi 1 / Msi1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- Y2709
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Musashi 1 also known as Msi1 is an RNA-binding protein with a mass of about 39 kDa. Msi1 is present in high levels in neural tissue and certain stem cells. It works mechanically by recognizing and binding to specific RNA sequences impacting the fate of the mRNA. Musashi 1 regulates the translation and stability of its target mRNA affecting protein synthesis at the post-transcriptional level. Its expression is not limited to neural tissues but is also detected in other tissues where stem or progenitor cells are present.
- Biological function summary
In neural stem cells and other tissue types Musashi 1 plays an important role in maintaining stem cell identity. Musashi 1 forms part of a complex that controls the translation of mRNAs involved in cell fate decisions. It acts by repressing or activating the translation of key regulatory proteins that guide stem cell maintenance and differentiation processes. This protein is also involved in cellular proliferation by influencing the translation of mRNAs tied to cell cycle regulation.
- Pathways
The function of Musashi 1 lies within critical signaling pathways such as Notch and Wnt. It facilitates these pathways by regulating the translation of downstream effectors which are necessary for cellular communication and differentiation. Musashi 1 associates with proteins like Numb a known Notch signaling inhibitor and β-catenin from the Wnt pathway modulating their effects on cell cycle progression and stem cell fate.
- Associated diseases and disorders
Musashi 1’s abnormal expression is often linked to conditions like glioblastoma and colorectal cancer. High levels of Musashi 1 can promote tumor growth due to its role in cell proliferation and differentiation pathways. In glioblastoma its expression relates closely with other oncogenic proteins such as Numb and Notch altering normal regulatory mechanisms. In colorectal cancer Musashi 1’s interaction with β-catenin further drives tumorigenesis highlighting its potential as a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--immunocytochemistry-immunofluorescence-img168538.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)")
Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
ab129819 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129819 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
![Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--western-blot-img104840.jpg "Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)")
Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
Lanes 1 - 3: Merged signal (red and green). Green - ab129819 observed at 39 kDa. Red - loading control, Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866, observed at 50 kDa.
ab129819 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. ab129819 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 5 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/mL
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: MSI1 knockout HAP1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y whole cell lysate at 20 µg
Predicted band size: 39 kDa
Observed band size: 39 kDa
![Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819), expandable thumbnail](https://content.abcam.com/products/images/musashi-1-msi1-antibody-y2709-ab129819--western-blot-img87652.jpg "Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)")
Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819)
All lanes: Western blot - Anti-Musashi 1 / Msi1 antibody [Y2709] (ab129819) at 5 µg/mL
Lane 1: Brain (Human) Membrane Lysate - fetal normal tissue (ab29457) at 20 µg
Lane 2: E14 Rat Embryo Brain Tissue Lysate at 20 µg
Lane 3: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 20 µg
Lane 4: 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 20 µg
SecondaryAll lanes: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa
Exposure time: 12min
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