Goat Polyclonal Muscarinic Acetylcholine Receptor 1/CHRM1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CHRM1 aa 400 to C-terminus.
Images
Publications
Filter by
- Life science alliance 7:2023IMPA1 dependent regulation of phosphatidylinositol 4,5-bisphosphate and calcium signalling by lithium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSankhanil Saha,Harini Krishnan,Padinjat RaghuPubMed 38056909
- Frontiers in neural circuits 15:7953252022Muscarinic Acetylcholine Receptor Localization on Distinct Excitatory and Inhibitory Neurons Within the ACC and LPFC of the Rhesus Monkey.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Tsolias,Maria MedallaPubMed 35087381
- Investigative ophthalmology & visual science 62:192021Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin Dankis,Thomas Carlsson,Patrik Aronsson,Gunnar Tobin,Michael WinderPubMed 34546325
- Basic & clinical pharmacology & toxicology 121:257-2652017Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael Winder et. al.PubMed 28437032
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CHRM1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11229
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Dog | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Pig, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Target data
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Alternative names
Muscarinic acetylcholine receptor M1, CHRM1
Recommended products
Goat Polyclonal Muscarinic Acetylcholine Receptor 1/CHRM1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CHRM1 aa 400 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CHRM1 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P11229
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab77098 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Muscarinic Acetylcholine Receptor 1 (CHRM1) is a G protein-coupled receptor that plays an important role in transmitting acetylcholine-mediated cholinergic signals. It also goes by the names 'M1 receptor' or 'muscarinic acetylcholine receptors'. The protein has a mass of approximately 51 kDa and is largely expressed in the central nervous system particularly in the cerebral cortex and hippocampus. Its activation leads to intracellular signaling cascades involving the modulation of ion channels and influencing cellular responses.
- Biological function summary
CHRM1 participates in various neural functions by interacting with acetylcholine. It does not form part of a complex itself but has significant interactions with other proteins particularly in nerve cells influencing cognitive processes and memory. CHRM1 plays an essential role in modulating neurotransmission which affects neuronal excitability and synaptic plasticity contributing to learning and attention processes.
- Pathways
CHRM1 is involved in the phosphoinositide pathway and modulation of the MAPK/ERK pathway. The receptor interacts with proteins like Gq and G11 family members during signal transduction. These pathways help mediate the cellular actions initiated by neurotransmitter binding therefore facilitating long-term potentiation and contributing to cognitive function and higher brain activities.
- Associated diseases and disorders
CHRM1 is associated with Alzheimer's disease and schizophrenia. Altered signaling or expression of this receptor often correlates with cognitive decline in Alzheimer's disease suggesting a significant role in neurodegenerative processes. In schizophrenia dysfunctional CHRM1 signaling may impact thought processes and behavioral symptoms. In these contexts CHRM1 is connected to proteins such as beta-amyloid in Alzheimer's and dopamine receptors in schizophrenia pointing to its relevance in these conditions' molecular pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098)
All lanes: Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody (ab77098) at 0.3 µg/mL
All lanes: human brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 51 kDa
Observed band size: 48 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com