Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240]

• WB

Lab

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (AB325432)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CHRM3 and CHRM5.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36054350).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(30-300KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (ab325432) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human CHRM1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human CHRM3 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human CHRM5 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 30-300 kDa,36 kDa

false

Exposure time: 48s

• WB

Lab

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (AB325432)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, kidney, cerebellum.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36054350).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

Exposure time : Lanes 1-4 : 59 seconds, lanes 5-7 : 180 seconds

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (ab325432) at 1/1000 dilution

Lane 1:

Mouse cerebral cortex tissue lysate at 50 µg

Lane 2:

Mouse kidney tissue lysate at 50 µg

Lane 3:

Mouse spleen tissue lysate at 50 µg

Lane 4:

Mouse cerebellum tissue lysate at 50 µg

Lane 5:

Human cerebral cortex tissue lysate at 50 µg

Lane 6:

Human spleen tissue lysate at 50 µg

Lane 7:

Human cerebellum tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,124 kDa

false

• WB

Lab

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (AB325432)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 36054350).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor 1/CHRM1 antibody [EPR26494-240] (ab325432) at 1/1000 dilution

Lane 1:

Rat cerebral cortex tissue lysate at 50 µg

Lane 2:

Rat spleen tissue lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,124 kDa

false

Exposure time: 48s