AB167566

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566) | Abcam
Brand: Abcam Limited
SKU: AB167566
Price: 535 USD
Availability: InStock

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(1 Publication)

Mouse Polyclonal Muscarinic Acetylcholine Receptor M3/CHRM3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CHRM3.

View Alternative Names

Muscarinic acetylcholine receptor M3, CHRM3

2 Images

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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB167566)

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 66 kDa

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Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB167566)

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab167566) at 1 µg/mL

Lane 1:

Muscarinic Acetylcholine Receptor M3/CHRM3 transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 66 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CHRM3.

P20309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscarinic Acetylcholine Receptor M3 also known as CHRM3 or M3 receptor plays an important role in various physiological processes. This receptor is part of the G-protein coupled receptor family and binds acetylcholine. The mass of CHRM3 is around 65 kDa. CHRM3 is widely expressed in various tissues including smooth muscle exocrine glands and in some cases the central nervous system. This extensive expression indicates its significance in multiple bodily functions.

Biological function summary

CHRM3 mediates cellular responses such as smooth muscle contraction and glandular secretion. It operates as part of a larger receptor complex that facilitates the signal transduction process. When acetylcholine binds to CHRM3 it activates downstream signaling pathways involving various intracellular messengers. This activation influences activities such as bronchoconstriction and salivation emphasizing the importance of M3 receptor in autonomic nervous system regulation.

Pathways

Studies show that CHRM3 engages with the inositol phospholipid-calcium signaling pathway. This pathway mobilizes calcium from intracellular stores working together with proteins like G-proteins and protein kinase C. These interactions lead to physiological effects such as muscle contraction and secretion. Moreover CHRM3 links with the MAPK/ERK pathway conveying signals that influence cell growth and differentiation. The intertwining of these pathways highlights CHRM3’s role in regulating key cellular functions.

CHRM3’s malfunction associates with asthma and chronic obstructive pulmonary disease (COPD). Dysregulated CHRM3 signaling can exacerbate symptoms involving bronchoconstriction and mucus secretion. Furthermore CHRM3 connects to proteins affecting airway smooth muscle cells intensifying disease conditions. Its relevance in exocrine gland regulation also implicates CHRM3 in conditions like Sjögren's syndrome where it may impact salivary and lacrimal gland secretion. Understanding these connections aids in developing targeted therapies for these ailments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

See full target information CHRM3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 11:2292-8 PubMed25412212

2014

Cyclic stretch induces human bladder smooth muscle cell proliferation in vitro through muscarinic receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Dai,Ye Tian,De-Yi Luo,Romel Wazir,Xuan Yue,Hong Li,Kun-Jie Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB167566)

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB167566)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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