AB60981

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab60981) | Abcam
Brand: Abcam Limited
SKU: AB60981
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M3/CHRM3 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHRM3.

View Alternative Names

Muscarinic acetylcholine receptor M3, CHRM3

1 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB60981)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, staining Muscarinic Acetylcholine Receptor M3/CHRM3 with ab60981 at 10 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRM3. The exact immunogen used to generate this antibody is proprietary information.

P20309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab60981 was purified by peptide immunogen affinity column.

Storage buffer

pH: 7.7 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscarinic Acetylcholine Receptor M3 also known as CHRM3 or M3 receptor plays an important role in various physiological processes. This receptor is part of the G-protein coupled receptor family and binds acetylcholine. The mass of CHRM3 is around 65 kDa. CHRM3 is widely expressed in various tissues including smooth muscle exocrine glands and in some cases the central nervous system. This extensive expression indicates its significance in multiple bodily functions.

Biological function summary

CHRM3 mediates cellular responses such as smooth muscle contraction and glandular secretion. It operates as part of a larger receptor complex that facilitates the signal transduction process. When acetylcholine binds to CHRM3 it activates downstream signaling pathways involving various intracellular messengers. This activation influences activities such as bronchoconstriction and salivation emphasizing the importance of M3 receptor in autonomic nervous system regulation.

Pathways

Studies show that CHRM3 engages with the inositol phospholipid-calcium signaling pathway. This pathway mobilizes calcium from intracellular stores working together with proteins like G-proteins and protein kinase C. These interactions lead to physiological effects such as muscle contraction and secretion. Moreover CHRM3 links with the MAPK/ERK pathway conveying signals that influence cell growth and differentiation. The intertwining of these pathways highlights CHRM3’s role in regulating key cellular functions.

CHRM3’s malfunction associates with asthma and chronic obstructive pulmonary disease (COPD). Dysregulated CHRM3 signaling can exacerbate symptoms involving bronchoconstriction and mucus secretion. Furthermore CHRM3 connects to proteins affecting airway smooth muscle cells intensifying disease conditions. Its relevance in exocrine gland regulation also implicates CHRM3 in conditions like Sjögren's syndrome where it may impact salivary and lacrimal gland secretion. Understanding these connections aids in developing targeted therapies for these ailments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

See full target information CHRM3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of comparative neurology 529:853-884 PubMed32656849

2020

Mapping of the excitatory, inhibitory, and modulatory afferent projections to the anatomically defined active expiratory oscillator in adult male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Vivian Biancardi,Jashan Saini,Anileen Pageni,Hema Prashaad M,Gregory D Funk,Silvia Pagliardini

Journal of vascular research 54:259-271 PubMed28810262

2017

Fetal Sheep Mesenteric Resistance Arteries: Functional and Structural Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Julia J Müller,Matthias Schwab,Charles R Rosenfeld,Iwa Antonow-Schlorke,Peter W Nathanielsz,Florian Rakers,Harald Schubert,Otto W Witte,Sven Rupprecht

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB60981)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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