AB87199

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199) | Abcam
Brand: Abcam Limited
SKU: AB87199
Price: 475 USD
Availability: InStock
Rating: 1 (1 reviews)

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(1 Review)

(13 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M3/CHRM3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 13 publications.

View Alternative Names

Chrm-3, Muscarinic acetylcholine receptor M3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

IHC image of Muscarinic Acetylcholine Receptor M3/CHRM3 staining in Rat normal brain (Sagittal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87199, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Unknown

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

Muscarinic Acetylcholine Receptor M3/CHRM3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (ab87199) at 1 µg/mL

Lane 1:

Brain (Rat) Tissue Lysate at 10 µg

Lane 2:

Rat Forebrain Rat Tissue Lysate at 10 µg

Lane 3:

Rat Substantia Nigra Tissue Lysate at 10 µg

Lane 4:

Heart (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 66 kDa

Observed band size: 16 kDa,85 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscarinic Acetylcholine Receptor M3 also known as CHRM3 or M3 receptor plays an important role in various physiological processes. This receptor is part of the G-protein coupled receptor family and binds acetylcholine. The mass of CHRM3 is around 65 kDa. CHRM3 is widely expressed in various tissues including smooth muscle exocrine glands and in some cases the central nervous system. This extensive expression indicates its significance in multiple bodily functions.

Biological function summary

CHRM3 mediates cellular responses such as smooth muscle contraction and glandular secretion. It operates as part of a larger receptor complex that facilitates the signal transduction process. When acetylcholine binds to CHRM3 it activates downstream signaling pathways involving various intracellular messengers. This activation influences activities such as bronchoconstriction and salivation emphasizing the importance of M3 receptor in autonomic nervous system regulation.

Pathways

Studies show that CHRM3 engages with the inositol phospholipid-calcium signaling pathway. This pathway mobilizes calcium from intracellular stores working together with proteins like G-proteins and protein kinase C. These interactions lead to physiological effects such as muscle contraction and secretion. Moreover CHRM3 links with the MAPK/ERK pathway conveying signals that influence cell growth and differentiation. The intertwining of these pathways highlights CHRM3’s role in regulating key cellular functions.

CHRM3’s malfunction associates with asthma and chronic obstructive pulmonary disease (COPD). Dysregulated CHRM3 signaling can exacerbate symptoms involving bronchoconstriction and mucus secretion. Furthermore CHRM3 connects to proteins affecting airway smooth muscle cells intensifying disease conditions. Its relevance in exocrine gland regulation also implicates CHRM3 in conditions like Sjögren's syndrome where it may impact salivary and lacrimal gland secretion. Understanding these connections aids in developing targeted therapies for these ailments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

See full target information Chrm3

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 17: PubMed39458894

2024

A Dataset for Constructing the Network Pharmacology of Overactive Bladder and Its Application to Reveal the Potential Therapeutic Targets of Rhynchophylline.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Tie,Jihan Liu,Yushan Wu,Yining Qiang,Ge'Er Cai'Li,Pingxiang Xu,Ming Xue,Liping Xu,Xiaorong Li,Xuelin Zhou

PLoS biology 22:e3002865 PubMed39436946

2024

The development of hepatic steatosis depends on the presence of liver-innervating parasympathetic cholinergic neurons in mice fed a high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Hwang,Junichi Okada,Li Liu,Jeffrey E Pessin,Gary J Schwartz,Young-Hwan Jo

Clinics (Sao Paulo, Brazil) 79:100485 PubMed39316895

2024

Exploration of the mechanism underlying the therapeutic effect of electroacupuncture at chengshan acupoint on post-hemorrhoidectomy anal pain: Insights from the mAChRs/IP3-Ca-CaM signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Song,Yang Wang,Ming Li,Yujuan Wang,Tianshu Xu

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Scientific reports 11:18094 PubMed34508162

2021

Carotid smooth muscle contractility changes after severe burn.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin DeSpain,Charles R Rosenfeld,Ryan Huebinger,Xiaofu Wang,Jayson W Jay,Ravi S Radhakrishnan,Steven E Wolf,Juquan Song

International journal of molecular sciences 22: PubMed34210091

2021

Adipose-Derived Stem Cells and Their Derived Microvesicles Ameliorate Detrusor Overactivity Secondary to Bilateral Partial Iliac Arterial Occlusion-Induced Bladder Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bing-Juin Chiang,Chun-Hou Liao,Su-Han Mao,Chiang-Ting Chien

Frontiers in pharmacology 11:01082 PubMed33013355

2020

Shugan Decoction Alleviates Colonic Dysmotility in Female SERT-Knockout Rats by Decreasing M Receptor Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yinshu Wang,Ying Dong,Enkang Wang,Yangyang Meng,Zijuan Bi,Shuai Sun,Chaochao Zhang,Haiting Fan,Jianye Yuan

Theranostics 9:4241-4254 PubMed31281545

2019

A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury.

Applications

Unspecified application

Species

Unspecified reactive species

Solaiman Tarafder,Christopher Ricupero,Sumeet Minhas,Rebecca J Yu,Ashleigh D Alex,Chang H Lee

Neurourology and urodynamics 37:2560-2570 PubMed30252154

2018

I-Tiao-Gung extract through its active component daidzin improves cyclophosphamide-induced bladder dysfunction in rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Kung-Chieh Wu,Bing-Juin Chiang,Wen-Hsin Tsai,Shiu-Dong Chung,Chiang-Ting Chien

American journal of physiology. Renal physiology 315:F45-F56 PubMed29092846

2017

BDNF overexpression in the bladder induces neuronal changes to mediate bladder overactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Mahendra P Kashyap,Subrata K Pore,William C de Groat,Christopher J Chermansky,Naoki Yoshimura,Pradeep Tyagi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

Western blot - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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