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AB77956

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2]

5

(2 Reviews)

|

(6 Publications)

Mouse Monoclonal Muscarinic Acetylcholine Receptor M4/CHRM4 antibody. Suitable for IP, Flow Cyt, WB, IHC-Fr and reacts with Mouse, Rat, Monkey, Human samples. Cited in 6 publications.

View Alternative Names

Muscarinic acetylcholine receptor M4, CHRM4

1 Images
Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (AB77956)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (AB77956)

Overlay histogram showing SH-SY5Y cells stained with ab77956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77956, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

18C7.2

Isotype

IgG1

Carrier free

No

Reacts with

Human, Rat, Mouse, Monkey

Applications

IHC-Fr, WB, Flow Cyt, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab77956 reacts with Muscarinic Acetylcholine Receptor M4/CHRM4. There is no reactivity with the other subtypes.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 1.45% Sodium chloride, 0.0536% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M4 also known as M4 receptor or CHRM4 is a G protein-coupled receptor with a mass of about 60-70 kDa. It is part of the muscarinic receptor family which includes five subtypes. Expression of CHRM4 occurs broadly in the central nervous system particularly within the brain's striatum and cortex regions influencing neuronal activity. The M4 muscarinic receptor plays a critical role in modulating neurotransmission responding to the neurotransmitter acetylcholine.
Biological function summary

The M4 muscarinic receptor influences several important processes by coupling with G proteins primarily inhibiting adenylate cyclase activity which results in reduced cyclic AMP levels. The M4 receptor is not generally part of a larger protein complex; rather it functions through interaction with associated proteins like Gi/Go proteins. This negative regulation can lead to various downstream effects that impact neural signaling making M4 receptors important in synaptic plasticity cognition and motor control.

Pathways

M4 muscarinic receptors participate significantly in the dopaminergic and cholinergic signaling pathways. M4 receptors interact closely with dopamine D1 receptors affecting dopamine release in the brain. This integration into these pathways highlights M4's role in motor and reward circuits showing its involvement alongside proteins such as other muscarinic receptors and dopamine-related proteins. These pathway involvements make it a target for pharmacological research in neurological contexts.

Alterations in M4 receptor function have connections to schizophrenia and Parkinson's disease. In schizophrenia imbalances of muscarinic receptors including M4 relate to cognitive deficits and are a focus for therapeutic strategies. Meanwhile in Parkinson's disease M4 receptors have been associated with motor control issues due to their role in modulating dopaminergic activity. Additionally studies point to the involvement of other muscarinic receptors and dopaminergic proteins in these disorders making M4 an important player in understanding and potentially treating these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
See full target information Muscarinic acetylcholine receptor M4
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 9:4241-4254 PubMed31281545

2019

A Combination of Oxo-M and 4-PPBP as a potential regenerative therapeutics for tendon injury.

Applications

Unspecified application

Species

Unspecified reactive species

Solaiman Tarafder,Christopher Ricupero,Sumeet Minhas,Rebecca J Yu,Ashleigh D Alex,Chang H Lee

Neuron 95:550-563.e5 PubMed28772121

2017

Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie R Thomson,Sang S Seo,Stephanie A Barnes,Susana R Louros,Melania Muscas,Owen Dando,Caoimhe Kirby,David J A Wyllie,Giles E Hardingham,Peter C Kind,Emily K Osterweil

eLife 5: PubMed27671734

2016

Accelerated remyelination during inflammatory demyelination prevents axonal loss and improves functional recovery.

Applications

IHC-FoFr

Species

Mouse

Feng Mei,Klaus Lehmann-Horn,Yun-An A Shen,Kelsey A Rankin,Karin J Stebbins,Daniel S Lorrain,Kara Pekarek,Sharon A Sagan,Lan Xiao,Cory Teuscher,H-Christian von Büdingen,Jürgen Wess,J Josh Lawrence,Ari J Green,Stephen Pj Fancy,Scott S Zamvil,Jonah R Chan

The European journal of neuroscience 44:1761-70 PubMed26990801

2016

Muscarinic control of rostromedial tegmental nucleus GABA neurons and morphine-induced locomotion.

Applications

Unspecified application

Species

Unspecified reactive species

David I Wasserman,Joel M J Tan,Jun Chul Kim,John S Yeomans

Frontiers in cellular neuroscience 10:71 PubMed27047336

2016

Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons.

Applications

IHC

Species

Unspecified reactive species

Zhe Zhao,Kang Zhang,Xiaoyan Liu,Haitao Yan,Xiaoyun Ma,Shuzhuo Zhang,Jianquan Zheng,Liyun Wang,Xiaoli Wei

Behavioural brain research 279:202-10 PubMed25451552

2014

Decreased dopamine receptor 1 activity and impaired motor-skill transfer in Dyt1 ΔGAG heterozygous knock-in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fumiaki Yokoi,Mai T Dang,Jun Liu,Jason R Gandre,Kelly Kwon,Robert Yuen,Yuqing Li
View all publications
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Alternative Products

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Secondary Antibodies

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Primary Antibodies

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Product promise

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