AB189432

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab189432) | Abcam
Brand: Abcam Limited
SKU: AB189432
Price: 630 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M4/CHRM4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 200-300.

View Alternative Names

Muscarinic acetylcholine receptor M4, CHRM4

2 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB189432)

Immunofluorescent staining of LOVO cells labeling Muscarinic Acetylcholine Receptor M4/CHRM4 using ab189432 at 1/100 dilution. Right panel is treated with the synthesized peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB189432)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB189432)

Immunohistochemical staining of Human testis tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 using ab189432 at 20 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P08173

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "20 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M4 also known as M4 receptor or CHRM4 is a G protein-coupled receptor with a mass of about 60-70 kDa. It is part of the muscarinic receptor family which includes five subtypes. Expression of CHRM4 occurs broadly in the central nervous system particularly within the brain's striatum and cortex regions influencing neuronal activity. The M4 muscarinic receptor plays a critical role in modulating neurotransmission responding to the neurotransmitter acetylcholine.

Biological function summary

The M4 muscarinic receptor influences several important processes by coupling with G proteins primarily inhibiting adenylate cyclase activity which results in reduced cyclic AMP levels. The M4 receptor is not generally part of a larger protein complex; rather it functions through interaction with associated proteins like Gi/Go proteins. This negative regulation can lead to various downstream effects that impact neural signaling making M4 receptors important in synaptic plasticity cognition and motor control.

Pathways

M4 muscarinic receptors participate significantly in the dopaminergic and cholinergic signaling pathways. M4 receptors interact closely with dopamine D1 receptors affecting dopamine release in the brain. This integration into these pathways highlights M4's role in motor and reward circuits showing its involvement alongside proteins such as other muscarinic receptors and dopamine-related proteins. These pathway involvements make it a target for pharmacological research in neurological contexts.

Alterations in M4 receptor function have connections to schizophrenia and Parkinson's disease. In schizophrenia imbalances of muscarinic receptors including M4 relate to cognitive deficits and are a focus for therapeutic strategies. Meanwhile in Parkinson's disease M4 receptors have been associated with motor control issues due to their role in modulating dopaminergic activity. Additionally studies point to the involvement of other muscarinic receptors and dopaminergic proteins in these disorders making M4 an important player in understanding and potentially treating these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

See full target information Muscarinic acetylcholine receptor M4

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 62:19 PubMed34546325

2021

Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Dankis,Thomas Carlsson,Patrik Aronsson,Gunnar Tobin,Michael Winder

Basic & clinical pharmacology & toxicology 121:257-265 PubMed28437032

2017

Autonomic Receptor-mediated Regulation of Production and Release of Nitric Oxide in Normal and Malignant Human Urothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Winder,Renata Vesela,Patrik Aronsson,Bhavik Patel,Thomas Carlsson

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB189432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB189432)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise