Rabbit Polyclonal Muscarinic Acetylcholine Receptor M4/CHRM4 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08173
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes Use at 1/50 - 1/200 dilution if using a fluorescent secondary antibody. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Use at 1/50 - 1/200 dilution if using a fluorescent secondary antibody. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Alternative names
Muscarinic acetylcholine receptor M4, CHRM4
Recommended products
Rabbit Polyclonal Muscarinic Acetylcholine Receptor M4/CHRM4 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P08173
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Muscarinic Acetylcholine Receptor M4 also known as M4 receptor or CHRM4 is a G protein-coupled receptor with a mass of about 60-70 kDa. It is part of the muscarinic receptor family which includes five subtypes. Expression of CHRM4 occurs broadly in the central nervous system particularly within the brain's striatum and cortex regions influencing neuronal activity. The M4 muscarinic receptor plays a critical role in modulating neurotransmission responding to the neurotransmitter acetylcholine.
- Biological function summary
The M4 muscarinic receptor influences several important processes by coupling with G proteins primarily inhibiting adenylate cyclase activity which results in reduced cyclic AMP levels. The M4 receptor is not generally part of a larger protein complex; rather it functions through interaction with associated proteins like Gi/Go proteins. This negative regulation can lead to various downstream effects that impact neural signaling making M4 receptors important in synaptic plasticity cognition and motor control.
- Pathways
M4 muscarinic receptors participate significantly in the dopaminergic and cholinergic signaling pathways. M4 receptors interact closely with dopamine D1 receptors affecting dopamine release in the brain. This integration into these pathways highlights M4's role in motor and reward circuits showing its involvement alongside proteins such as other muscarinic receptors and dopamine-related proteins. These pathway involvements make it a target for pharmacological research in neurological contexts.
- Associated diseases and disorders
Alterations in M4 receptor function have connections to schizophrenia and Parkinson's disease. In schizophrenia imbalances of muscarinic receptors including M4 relate to cognitive deficits and are a focus for therapeutic strategies. Meanwhile in Parkinson's disease M4 receptors have been associated with motor control issues due to their role in modulating dopaminergic activity. Additionally studies point to the involvement of other muscarinic receptors and dopaminergic proteins in these disorders making M4 an important player in understanding and potentially treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab214458)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab214458)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab214458)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, PE conjugated secondary antibody at 1/200 dilution.
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