AB214458

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (ab214458) | Abcam
Brand: Abcam Limited
SKU: AB214458
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M4/CHRM4 antibody. Suitable for IHC-P and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Muscarinic acetylcholine receptor M4, CHRM4

3 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling Muscarinic Acetylcholine Receptor M4/CHRM4 with ab214458 at 1/200 dilution, followed by Goat Anti-Rabbit IgG, PE conjugated secondary antibody at 1/200 dilution.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Muscarinic acetylcholine receptor M4 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P08173

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 dilution if using a fluorescent secondary antibody.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>Use at 1/50 - 1/200 dilution if using a fluorescent secondary antibody.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M4 also known as M4 receptor or CHRM4 is a G protein-coupled receptor with a mass of about 60-70 kDa. It is part of the muscarinic receptor family which includes five subtypes. Expression of CHRM4 occurs broadly in the central nervous system particularly within the brain's striatum and cortex regions influencing neuronal activity. The M4 muscarinic receptor plays a critical role in modulating neurotransmission responding to the neurotransmitter acetylcholine.

Biological function summary

The M4 muscarinic receptor influences several important processes by coupling with G proteins primarily inhibiting adenylate cyclase activity which results in reduced cyclic AMP levels. The M4 receptor is not generally part of a larger protein complex; rather it functions through interaction with associated proteins like Gi/Go proteins. This negative regulation can lead to various downstream effects that impact neural signaling making M4 receptors important in synaptic plasticity cognition and motor control.

Pathways

M4 muscarinic receptors participate significantly in the dopaminergic and cholinergic signaling pathways. M4 receptors interact closely with dopamine D1 receptors affecting dopamine release in the brain. This integration into these pathways highlights M4's role in motor and reward circuits showing its involvement alongside proteins such as other muscarinic receptors and dopamine-related proteins. These pathway involvements make it a target for pharmacological research in neurological contexts.

Alterations in M4 receptor function have connections to schizophrenia and Parkinson's disease. In schizophrenia imbalances of muscarinic receptors including M4 relate to cognitive deficits and are a focus for therapeutic strategies. Meanwhile in Parkinson's disease M4 receptors have been associated with motor control issues due to their role in modulating dopaminergic activity. Additionally studies point to the involvement of other muscarinic receptors and dopaminergic proteins in these disorders making M4 an important player in understanding and potentially treating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

See full target information Muscarinic acetylcholine receptor M4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Gynecologic oncology 131:309-14 PubMed23938375

2013

MMR deficiency is common in high-grade endometrioid carcinomas and is associated with an unfavorable outcome.

Applications

Unspecified application

Species

Unspecified reactive species

Gregg S Nelson,Aaron Pink,Sandra Lee,Guangming Han,Don Morris,Travis Ogilvie,Máire A Duggan,Martin Köbel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody (AB214458)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise