AB150531

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab150531) | Abcam
Brand: Abcam Limited
SKU: AB150531

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(2 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M5/CHRM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CHRM5.

View Alternative Names

Muscarinic acetylcholine receptor M5, CHRM5

1 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB150531)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue (vessel) labeling Muscarinic Acetylcholine Receptor M5/CHRM5 with ab150531 at 6 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CHRM5. The exact immunogen used to generate this antibody is proprietary information.

P08912

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M5 also known as CHRM5 or the M5 receptor acts as a G protein-coupled receptor. This receptor binds acetylcholine a neurotransmitter and plays an important role in modulating neural activity. The M5 receptor has a molecular mass of approximately 61 kDa. Expression of the M5 receptor is found mainly in the brain areas such as the substantia nigra and midbrain dopaminergic neurons as well as in the vascular endothelium.

Biological function summary

The M5 receptor influences various physiological processes through its receptor activity. It affects functions like dopamine release which is important for neural pathways involved in reward and addiction. The M5 receptor does not operate as part of a larger receptor complex but interacts functionally with other muscarinic receptor subtypes contributing to the modulation of central nervous system activities.

Pathways

The M5 receptor plays a role in the dopaminergic signaling pathway and the phosphoinositide pathway. These pathways are important for regulating neurotransmission and vasodilation. Other proteins it interacts with in these pathways include D1-like dopamine receptors which collaborate in controlling neuronal excitability and blood flow.

The M5 receptor is linked to conditions such as addiction and schizophrenia. In addiction it interacts with the dopaminergic system while in schizophrenia altered M5 receptor function affects neural circuits. Furthermore its interaction with dopamine receptors highlights its potential involvement in these neurological disorders suggesting therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

See full target information CHRM5

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular oncology 18:386-414 PubMed37842807

2023

Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Yu Hsieh,Jia-Ling Du,Pei-Ming Yang

Oncology letters 16:775-784 PubMed29963145

2018

Muscarinic cholinergic signaling and overactive bladder-like symptoms associated with invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Minggang Wang,Yunglong Li,Qinggui Meng,Yong Tang,Haoyuan Lu,Wenchao Yu,Qiwei Cheng,You Li,Long Xu,Shaojun Jian,Yuexian Wu,Xianlin Yi,Keji Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB150531)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise