Rabbit Polyclonal Muscarinic Acetylcholine Receptor M5/CHRM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CHRM5.
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- Oncology letters 16:775-7842018Muscarinic cholinergic signaling and overactive bladder-like symptoms associated with invasive bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Wei et. al.PubMed 29963145
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CHRM5. The exact immunogen used to generate this antibody is proprietary information. Database link P08912
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Gorilla | Predicted |
| Monkey | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Gorilla | Dilution info - | Notes - |
Associated Products
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Target data
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Alternative names
Muscarinic acetylcholine receptor M5, CHRM5
Recommended products
Rabbit Polyclonal Muscarinic Acetylcholine Receptor M5/CHRM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CHRM5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CHRM5. The exact immunogen used to generate this antibody is proprietary information. Database link P08912
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Muscarinic Acetylcholine Receptor M5 also known as CHRM5 or the M5 receptor acts as a G protein-coupled receptor. This receptor binds acetylcholine a neurotransmitter and plays an important role in modulating neural activity. The M5 receptor has a molecular mass of approximately 61 kDa. Expression of the M5 receptor is found mainly in the brain areas such as the substantia nigra and midbrain dopaminergic neurons as well as in the vascular endothelium.
- Biological function summary
The M5 receptor influences various physiological processes through its receptor activity. It affects functions like dopamine release which is important for neural pathways involved in reward and addiction. The M5 receptor does not operate as part of a larger receptor complex but interacts functionally with other muscarinic receptor subtypes contributing to the modulation of central nervous system activities.
- Pathways
The M5 receptor plays a role in the dopaminergic signaling pathway and the phosphoinositide pathway. These pathways are important for regulating neurotransmission and vasodilation. Other proteins it interacts with in these pathways include D1-like dopamine receptors which collaborate in controlling neuronal excitability and blood flow.
- Associated diseases and disorders
The M5 receptor is linked to conditions such as addiction and schizophrenia. In addiction it interacts with the dopaminergic system while in schizophrenia altered M5 receptor function affects neural circuits. Furthermore its interaction with dopamine receptors highlights its potential involvement in these neurological disorders suggesting therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab150531)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain tissue (vessel) labeling Muscarinic Acetylcholine Receptor M5/CHRM5 with ab150531 at 6 μg/ml.
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