AB186830

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Name: Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab186830) | Abcam
Brand: Abcam Limited
SKU: AB186830
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M5/CHRM5 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHRM5 aa 200-450.

View Alternative Names

Muscarinic acetylcholine receptor M5, CHRM5

2 Images

Supplier Data

Western blot - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

All lanes:

Western blot - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (ab186830) at 1/500 dilution

Lane 1:

BT474 cell extract

Lane 2:

U251 cell extract

Lane 3:

Mouse liver extract

Predicted band size: 60 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186830. Blue DAPI for nuclear staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human CHRM5 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

P08912

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M5 also known as CHRM5 or the M5 receptor acts as a G protein-coupled receptor. This receptor binds acetylcholine a neurotransmitter and plays an important role in modulating neural activity. The M5 receptor has a molecular mass of approximately 61 kDa. Expression of the M5 receptor is found mainly in the brain areas such as the substantia nigra and midbrain dopaminergic neurons as well as in the vascular endothelium.

Biological function summary

The M5 receptor influences various physiological processes through its receptor activity. It affects functions like dopamine release which is important for neural pathways involved in reward and addiction. The M5 receptor does not operate as part of a larger receptor complex but interacts functionally with other muscarinic receptor subtypes contributing to the modulation of central nervous system activities.

Pathways

The M5 receptor plays a role in the dopaminergic signaling pathway and the phosphoinositide pathway. These pathways are important for regulating neurotransmission and vasodilation. Other proteins it interacts with in these pathways include D1-like dopamine receptors which collaborate in controlling neuronal excitability and blood flow.

The M5 receptor is linked to conditions such as addiction and schizophrenia. In addiction it interacts with the dopaminergic system while in schizophrenia altered M5 receptor function affects neural circuits. Furthermore its interaction with dopamine receptors highlights its potential involvement in these neurological disorders suggesting therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

See full target information CHRM5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Chemistry & biodiversity 22:e202402771 PubMed39776239

2025

Protocatechuic Acid Improves Alzheimer's Disease by Regulating the Cholinergic Synaptic Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Siwen Li,Songtao Li,Rasmané Semde,Hongbo Teng,Mengqi Shi,Liang Huang,Xinru Lou,Beining Jia,Hongyan Zhu,Yan Zhao

Medical principles and practice : international journal of the Kuwait University, Health Science Centre 33:56-65 PubMed38029727

2023

NSUN6 Regulates NM23-H1 Expression in an m5C Manner to Affect Epithelial-Mesenchymal Transition in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhansheng Lu,Bo Liu,Demiao Kong,Xiaojiang Zhou,Dengke Pei,Di Liu

Clinical and translational medicine 12:e890 PubMed35758323

2022

Phosphoproteome reveals molecular mechanisms of aberrant rhythm in neurotransmitter-mediated islet hormone secretion in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiang He,Qi Fu,Min Sun,Yu Qian,Yucheng Liang,Jie Zhang,Rui Gao,Hemin Jiang,Hao Dai,Yuwei Liu,Xinyu Xu,Heng Chen,Kuanfeng Xu,Tao Yang

Communications biology 5:495 PubMed35614315

2022

Nsun4 and Mettl3 mediated translational reprogramming of Sox9 promotes BMSC chondrogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Zhenxing Ren,Shenyu Yan,Ling Zhao,Jie Liu,Lijun Zhao,Zhen Li,Shanyu Ye,Aijun Liu,Xichan Li,Jiasong Guo,Wei Zhao,Weihong Kuang,Helu Liu,Dongfeng Chen

Investigative ophthalmology & visual science 62:19 PubMed34546325

2021

Novel Insights Into Muscarinic and Purinergic Responses in Primary Cultures of Rat Lacrimal Gland Myoepithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Dankis,Thomas Carlsson,Patrik Aronsson,Gunnar Tobin,Michael Winder

Scientific reports 10:6429 PubMed32286426

2020

Discovery of novel biomarkers for atherosclerotic aortic aneurysm through proteomics-based assessment of disease progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Yagi,Mitsuhiro Nishigori,Yusuke Murakami,Tsukasa Osaki,Sayaka Muto,Yutaka Iba,Kenji Minatoya,Yoshihiko Ikeda,Hatsue Ishibashi-Ueda,Takayuki Morisaki,Hitoshi Ogino,Hiroshi Tanaka,Hiroaki Sasaki,Hitoshi Matsuda,Naoto Minamino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Western blot - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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