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AB41171

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

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(6 Publications)

Rabbit Polyclonal Muscarinic Acetylcholine Receptor M5/CHRM5 antibody. Suitable for ELISA, WB, IHC-FoFr, IHC (PFA fixed) and reacts with Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Rat Chrm5.

View Alternative Names

Chrm-5, Muscarinic acetylcholine receptor M5

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-FoFr, ELISA, WB, IHC (PFA fixed)

applications

Immunogen

Synthetic Peptide within Rat Chrm5. The exact immunogen used to generate this antibody is proprietary information.

P08911

Specificity

Cross reactivity: M5 Muscarinic Receptor (519-531): 100% M5 Muscarinic Receptor: 75% No cross reactivity was observed in: M1 Muscarinic Receptor(451-460) M1 Muscarinic Receptor M2 Muscarinic Receptor(457-466) M2 Muscarinic Receptor M3 Muscarinic Receptor(580-589) M3 Muscarinic Receptor M4 Muscarinic Receptor(469-478) M4 Muscarinic Receptor

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Alternative Products

Primary Antibodies

AB150531

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB150531)

  • 0
  • 0
Primary Antibodies

AB186830

Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor M5/CHRM5 antibody (AB186830)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB87199

Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody (AB87199)

  • 1
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Biochemicals

AB120117

QX-314 bromide (N-Ethyllidocaine bromide), Na+ channel blocker

Chemical Structure - QX-314 bromide (N-Ethyllidocaine bromide), Na+ channel blocker (AB120117)

  • 0
  • 0
Primary Antibodies

AB77956

Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2]

Flow Cytometry - Anti-Muscarinic Acetylcholine Receptor M4/CHRM4 antibody [18C7.2] (AB77956)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Blocking with 5% goat or donkey serum significantly reduces background when compared with BSA or milk blocking.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in PBS, 10 mg/mL BSA, 0.01% sodium azide
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muscarinic Acetylcholine Receptor M5 also known as CHRM5 or the M5 receptor acts as a G protein-coupled receptor. This receptor binds acetylcholine a neurotransmitter and plays an important role in modulating neural activity. The M5 receptor has a molecular mass of approximately 61 kDa. Expression of the M5 receptor is found mainly in the brain areas such as the substantia nigra and midbrain dopaminergic neurons as well as in the vascular endothelium.
Biological function summary

The M5 receptor influences various physiological processes through its receptor activity. It affects functions like dopamine release which is important for neural pathways involved in reward and addiction. The M5 receptor does not operate as part of a larger receptor complex but interacts functionally with other muscarinic receptor subtypes contributing to the modulation of central nervous system activities.

Pathways

The M5 receptor plays a role in the dopaminergic signaling pathway and the phosphoinositide pathway. These pathways are important for regulating neurotransmission and vasodilation. Other proteins it interacts with in these pathways include D1-like dopamine receptors which collaborate in controlling neuronal excitability and blood flow.

The M5 receptor is linked to conditions such as addiction and schizophrenia. In addiction it interacts with the dopaminergic system while in schizophrenia altered M5 receptor function affects neural circuits. Furthermore its interaction with dopamine receptors highlights its potential involvement in these neurological disorders suggesting therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
See full target information Chrm5
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 15:662216 PubMed33897375

2021

Acetylcholine Receptor Stimulation Activates Protein Kinase C Mediated Internalization of the Dopamine Transporter.

Applications

Unspecified application

Species

Unspecified reactive species

Suzanne M Underhill,Susan G Amara

Nature communications 8:1676 PubMed29162816

2017

Impairments of spatial memory in an Alzheimer's disease model via degeneration of hippocampal cholinergic synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Houze Zhu,Huanhuan Yan,Na Tang,Xinyan Li,Pei Pang,Hao Li,Wenting Chen,Yu Guo,Shu Shu,You Cai,Lei Pei,Dan Liu,Min-Hua Luo,Hengye Man,Qing Tian,Yangling Mu,Ling-Qiang Zhu,Youming Lu

Data in brief 10:482-486 PubMed28054012

2017

Localization of muscarinic acetylcholine receptor 2 to the intestinal crypt stem cell compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Eleanor D Muise,Neeru Gandotra,John J Tackett,Michaela C Bamdad,Robert A Cowles

PloS one 10:e0139726 PubMed26431344

2015

Sympathetic Neurotransmitters Modulate Osteoclastogenesis and Osteoclast Activity in the Context of Collagen-Induced Arthritis.

Applications

IF

Species

Unspecified reactive species

Dominique Muschter,Nicole Schäfer,Hubert Stangl,Rainer H Straub,Susanne Grässel

Arthritis research & therapy 17:169 PubMed26104678

2015

Reactivity of rat bone marrow-derived macrophages to neurotransmitter stimulation in the context of collagen II-induced arthritis.

Applications

IF

Species

Unspecified reactive species

Dominique Muschter,Claudia Göttl,Mandy Vogel,Joachim Grifka,Rainer H Straub,Susanne Grässel

The European journal of neuroscience 38:2774-85 PubMed23773170

2013

Cholinergic control of morphine-induced locomotion in rostromedial tegmental nucleus versus ventral tegmental area sites.

Applications

IHC-FoFr

Species

Mouse

David I Wasserman,Haoran G Wang,Asim J Rashid,Sheena A Josselyn,John S Yeomans
View all publications
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Product promise

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