AB7813

Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker

Name: Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (ab7813) | Abcam
Brand: Abcam Limited
SKU: AB7813
Price: 485 USD
Availability: InStock

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(8 Publications)

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human ACTA1.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (AB7813)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (AB7813)

ab7813 staining human normal skeletal muscle. Staining is localized to the cytoplasm.
Left panel : with primary antibody diluted 1 : 4000. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (AB7813)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle labelling muscle Actin with ab7813.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HHF35

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human ACTA1.

P68133

Specificity

This antibody recognizes muscle specific alpha and gamma actin isomers but is non-reactive with beta isomers.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/75", "IHCP-species-notes": "<p>Prolonged fixation in buffered formalin can destroy the epitope.</p>" } } }

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Product details

This antibody stains myocardial, skeletal muscle and smooth muscle cells as well as myoepithelial cells, pericytes of small vessels. All other non-muscle cells were negative.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscle Actin also known as ACTA1 is a protein that plays a significant role in muscle contraction. It has a molecular weight of approximately 42 kDa. This protein is primarily found in skeletal muscle tissues. Muscle Actin exists in two forms: G-actin a globular monomer and F-actin a filamentous polymer that forms actin filaments. These filaments provide structural support and are involved in muscle contraction through their interaction with myosin.

Biological function summary

Muscle Actin interacts with myosin to facilitate muscle contraction. It is an important component of the thin filament within the sarcomere the basic unit of muscle fiber. Muscle Actin is part of a complex and this complex includes other important proteins like tropomyosin and troponin. These proteins regulate the interaction between actin and myosin enabling the muscle contraction process. Muscle Actin's polymerization and depolymerization dynamics are important for the regulation of cellular movement and structural integrity.

Pathways

Muscle Actin is essential for the actin-myosin contractile machinery in muscle cells which is part of the sarcomere assembly pathway. This process involves other proteins such as myosin heavy chain and tropomyosin which are important for muscle contraction. Another important pathway is the Rho GTPase signaling pathway which controls actin filament assembly and is involved in a variety of cellular processes including muscle contraction and cell motility.

Muscle Actin mutations link to certain muscle-related diseases such as nemaline myopathy and congenital myopathies. Nemaline myopathy involves the presence of rod-like structures in muscle fibers and it results from anomalies in actin filaments. Muscle Actin also associates with cardiac muscle conditions when there are mutations affecting cardiac actin structure or function. This reveals the connection between Muscle Actin tropomyosin and troponin in muscle contraction efficiency and structural integrity contributing to these diseases' pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Neurobiology of disease 194:106474 PubMed38518837

2024

Engrailed 1 deficiency induces changes in ciliogenesis during human neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Hembach,Sebastian Schmidt,Tanja Orschmann,Ingo Burtscher,Heiko Lickert,Florian Giesert,Daniela Vogt Weisenhorn,Wolfgang Wurst

Oncology letters 15:3937-3943 PubMed29456742

2018

miR302a inhibits the proliferation of esophageal cancer cells through the MAPK and PI3K/Akt signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Daokui Xia,Shumei Tian,Zhen Chen,Wenchao Qin,Quan Liu

Journal of cell science 127:583-98 PubMed24284066

2013

Live cell imaging reveals actin-cytoskeleton-induced self-association of the actin-bundling protein WLIM1.

Applications

Species

Human

Céline Hoffmann,Danièle Moes,Monika Dieterle,Katrin Neumann,Flora Moreau,Angela Tavares Furtado,Dominique Dumas,André Steinmetz,Clément Thomas

The Journal of clinical investigation 117:3248-57 PubMed17948129

2007

Derivation of sarcomas from mesenchymal stem cells via inactivation of the Wnt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Matushansky,Eva Hernando,Nicholas D Socci,Joslyn E Mills,Tulio A Matos,Mark A Edgar,Samuel Singer,Robert G Maki,Carlos Cordon-Cardo

The American journal of pathology 131:19-28 PubMed3354641

1988

Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins.

Applications

Unspecified application

Species

Unspecified reactive species

R A Schmidt,R Cone,J E Haas,A M Gown

The American journal of pathology 130:205-15 PubMed3276210

1988

Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors.

Applications

Unspecified application

Species

Unspecified reactive species

M Miettinen

The American journal of pathology 127:389-402 PubMed3555106

1987

HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues.

Applications

Unspecified application

Species

Unspecified reactive species

T Tsukada,M A McNutt,R Ross,A M Gown

The American journal of pathology 126:51-60 PubMed3544852

1987

HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.

Applications

Unspecified application

Species

Unspecified reactive species

T Tsukada,D Tippens,D Gordon,R Ross,A M Gown

View all publications

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Product promise

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Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (AB7813)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-muscle Actin antibody [HHF35] - Cytoskeleton Marker (AB7813)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise