AB216039

Anti-muscle Actin antibody [MSA/953] - Loading Control

Name: Anti-muscle Actin antibody [MSA/953] - Loading Control (ab216039) | Abcam
Brand: Abcam Limited
SKU: AB216039
Price: 495 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal alpha skeletal muscle Actin antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ACTA1.

View Alternative Names

ACTA, ACTA1, Alpha-actin-1

1 Images

Supplier Data

Western blot - Anti-muscle Actin antibody [MSA/953] - Loading Control (AB216039)

All lanes:

Western blot - Anti-muscle Actin antibody [MSA/953] - Loading Control (ab216039) at 1 µg/mL

All lanes:

hSKM cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MSA/953

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ACTA1. The exact immunogen used to generate this antibody is proprietary information.

P68133

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-5 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT).", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-5 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT).", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab216039 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Muscle Actin also known as ACTA1 is a protein that plays a significant role in muscle contraction. It has a molecular weight of approximately 42 kDa. This protein is primarily found in skeletal muscle tissues. Muscle Actin exists in two forms: G-actin a globular monomer and F-actin a filamentous polymer that forms actin filaments. These filaments provide structural support and are involved in muscle contraction through their interaction with myosin.

Biological function summary

Muscle Actin interacts with myosin to facilitate muscle contraction. It is an important component of the thin filament within the sarcomere the basic unit of muscle fiber. Muscle Actin is part of a complex and this complex includes other important proteins like tropomyosin and troponin. These proteins regulate the interaction between actin and myosin enabling the muscle contraction process. Muscle Actin's polymerization and depolymerization dynamics are important for the regulation of cellular movement and structural integrity.

Pathways

Muscle Actin is essential for the actin-myosin contractile machinery in muscle cells which is part of the sarcomere assembly pathway. This process involves other proteins such as myosin heavy chain and tropomyosin which are important for muscle contraction. Another important pathway is the Rho GTPase signaling pathway which controls actin filament assembly and is involved in a variety of cellular processes including muscle contraction and cell motility.

Muscle Actin mutations link to certain muscle-related diseases such as nemaline myopathy and congenital myopathies. Nemaline myopathy involves the presence of rod-like structures in muscle fibers and it results from anomalies in actin filaments. Muscle Actin also associates with cardiac muscle conditions when there are mutations affecting cardiac actin structure or function. This reveals the connection between Muscle Actin tropomyosin and troponin in muscle contraction efficiency and structural integrity contributing to these diseases' pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

Additional targets

ACTA2,ACTC1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 218: PubMed33779682

2021

Inhibition of macrophage histone demethylase JMJD3 protects against abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Frank M Davis,Lam C Tsoi,William J Melvin,Aaron denDekker,Rachael Wasikowski,Amrita D Joshi,Sonya Wolf,Andrea T Obi,Allison C Billi,Xianying Xing,Christopher Audu,Bethany B Moore,Steven L Kunkel,Alan Daugherty,Hong S Lu,Johann E Gudjonsson,Katherine A Gallagher

Orthopaedic surgery 13:616-622 PubMed33620145

2021

H3.3 K36M Mutation as a Clinical Diagnosis Method of Suspected Chondroblastoma Cases.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Mu,Yafei Jiang,Linghang Xue,Yingqi Hua,Jun Lin,Zhengdong Cai

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-muscle Actin antibody [MSA/953] - Loading Control

Western blot - Anti-muscle Actin antibody [MSA/953] - Loading Control (AB216039)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise