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AB249859

Anti-Muted antibody [EPR11081(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Muted antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

MUTED, BLOC1S5, Biogenesis of lysosome-related organelles complex 1 subunit 5, BLOC-1 subunit 5, Protein Muted homolog

2 Images
Immunoprecipitation - Anti-Muted antibody [EPR11081(B)] - BSA and Azide free (AB249859)
  • IP

Supplier Data

Immunoprecipitation - Anti-Muted antibody [EPR11081(B)] - BSA and Azide free (AB249859)

This data was developed using ab175205, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling Muted with ab175205 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Muted antibody [EPR11081(B)] (<a href='/en-us/products/primary-antibodies/muted-antibody-epr11081b-ab175205'>ab175205</a>)

Predicted band size: 22 kDa

false

Western blot - Anti-Muted antibody [EPR11081(B)] - BSA and Azide free (AB249859)
  • WB

Supplier Data

Western blot - Anti-Muted antibody [EPR11081(B)] - BSA and Azide free (AB249859)

This data was developed using ab175205, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Muted antibody [EPR11081(B)] (<a href='/en-us/products/primary-antibodies/muted-antibody-epr11081b-ab175205'>ab175205</a>) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 22 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11081(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249859 is the carrier-free version of ab175205.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Muted protein also known as MUTED plays a role in the trafficking and sorting of proteins in vesicles. This small protein with an approximate molecular mass of 22 kDa mainly expresses in melanocytes but researchers have also observed it in other cell types. It functions closely with other proteins involved in the regulation of melanosome biology. The expression pattern of Muted suggests it has specific roles in pigmentation and possibly related cellular processes.
Biological function summary

Muted influences important cellular functions by forming part of the biogenesis of lysosome-related organelles complex-1 (BLOC-1). This complex which includes eight subunits is vital for the generation of lysosome-related organelles such as melanosomes and platelet-dense granules. Muted's role in the complex affects the specialized organelles essential for pigmentation and other related cellular processes. Without proper functioning of BLOC-1 various cellular activities reliant on functional organelles face impairment.

Pathways

Muted and other molecules participate in the endosomal-lysosomal pathway and the melanosome biogenesis pathway. These pathways are important for the formation of lysosome-related organelles. Alongside proteins like Pallidin and Cappuccino Muted influences the movement and formation of vesicles required for transporting cargos to their destination within cells. Proper coordination within these pathways ensures cellular components reach the right location influencing pigmentation and other cell-specific functions.

Muted's malfunction associates with conditions such as Hermansky-Pudlak syndrome and oculocutaneous albinism type 2. These disorders result from defects in the biogenesis of lysosome-related organelles. Muted and associated proteins like Melanophilin and Myosin Va guide the transport mechanisms impaired in these disease states leading to characteristic symptoms like hypopigmentation and bleeding disorders. Understanding the Muted protein helps in deciphering the molecular basis of these conditions and aids in the development of potential interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes (PubMed : 32565547). In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking.
See full target information BLOC1S5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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