Anti-MVP antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody (AB226248)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for MVP using ab226248 at 1/5000 dilution in immunohistochemical analysis.

Detection : DAB staining.

• IP

Supplier Data

Immunoprecipitation - Anti-MVP antibody (AB226248)

MVP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226248 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226248 at 0.4 μg/ml.

Lane 1 : ab226248 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-MVP antibody (ab226248)

Predicted band size: 99 kDa

false

• WB

Supplier Data

Western blot - Anti-MVP antibody (AB226248)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-MVP antibody (ab226248) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 99 kDa

true

Exposure time: 10s