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Rabbit Recombinant Monoclonal MVP antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-MVP antibody [EPR13226(B)] (AB177145), expandable thumbnail
  • Western blot - Anti-MVP antibody [EPR13226(B)] (AB177145), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWB
Human
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Predicted
Rat
Not recommended
Not recommended
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Alternative names

LRP, MVP, Major vault protein, Lung resistance-related protein

Recommended products

Rabbit Recombinant Monoclonal MVP antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR13226(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145), expandable thumbnail

    Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)

    ab177145 Anti-MVP antibody [EPR13226(B)] was shown to specifically react with MVP in HeLa wild-type cells ( ab255448). Loss of signal was observed when knockout cell line Human MVP knockout HeLa cell line ab264817 (knockout cell lysate Human MVP knockout HeLa cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab177145 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

    Lane 1: Wild-type HeLa lysate at 20 µg

    Lane 2: MVP knockout HeLa lysate at 20 µg

    Lane 2: Western blot - Human MVP knockout HeLa cell line (Human MVP knockout HeLa cell line ab264817)

    Lane 3: A549 lysate at 20 µg

    Lane 4: MOLT-4 lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 99 kDa

    Observed band size: 99 kDa

  • Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145), expandable thumbnail

    Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145)

    All lanes: Western blot - Anti-MVP antibody [EPR13226(B)] (ab177145) at 1/1000 dilution

    Lane 1: Human spleen lysate at 10 µg

    Lane 2: Calu3 lysate at 10 µg

    Lane 3: A549 lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 99 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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