Anti-MVP antibody [EPR13227(B)] (ab175239)

Rabbit Recombinant Monoclonal MVP antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

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Images

Western blot - Anti-MVP antibody [EPR13227(B)] (AB175239), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50 - 1/350	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes For unpurified use at 1/10000 - 1/50000.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/150 - 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/500	Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

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3 products for Alternative Product

Target data

See full target information MVP

Function

Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.

Alternative names

LRP, MVP, Major vault protein, Lung resistance-related protein

Recommended products

Rabbit Recombinant Monoclonal MVP antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR13227(B)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)
ab175239 Anti-MVP antibody [EPR13227(B)] was shown to specifically react with MVP in HeLa wild-type cells ( ab255448). Loss of signal was observed when knockout cell line Human MVP knockout HeLa cell line ab264817 (knockout cell lysate Human MVP knockout HeLa cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. ab175239 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/2000 dilution
Lane 1: Wild-type HeLa lysate at 20 µg
Lane 2: MVP knockout HeLa lysate at 20 µg
Lane 2: Western blot - Human MVP knockout HeLa cell line (Human MVP knockout HeLa cell line ab264817)
Lane 3: A549 lysate at 20 µg
Lane 4: MOLT-4 lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] (ab175239)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MVP with purified ab175239 at a dilution of 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/2000 dilution
All lanes: Caco-2 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)
Blocking and dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/10000 dilution
Lane 1: Calu-3 whole cell lysate at 20 µg
Lane 2: A549 whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 53 kDa, 55 kDa, 73 kDa, 97 kDa, 99 kDa
Observed band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MVP with purified ab175239 at a dilution of 1/350. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] (ab175239)
Intracellular Flow Cytometry analysis of A549 cells labelling MVP with purified ab175239 at a dilution of 1/180 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)
All lanes: Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239) at 1/10000 dilution
Lane 1: A549 cell lysate at 10 µg
Lane 2: Calu-3 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 99 kDa
Observed band size: 110 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] (ab175239)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling MVP with unpurified ab175239 at a dilution of 1/50.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] (ab175239)
Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling MVP with unpurified ab175239 at a dilution of 1/250 (red) and DAPI staining (blue).
Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] (ab175239)
Intracellular flow cytometrical analysis of permeabilized A549 cells labeling MVP with unpurified ab175239 antibody at a dilution of 1/100 (red) compared to a negative control (Rabbit IgG, green).
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-MVP antibody [EPR13227(B)] (ab175239)
MVP western blot using anti-MVP antibody [EPR13227(B)] ab175239. Publication image and figure legend from Shen, W., Qiu, Y., et al., 2019, Cancer Med, PubMed 31044552.

ab175239 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab175239 please see the product overview.
IL‐25 regulates the NF‐κB signaling pathway activity. A, The change of NF‐κB signaling pathway in IL‐25 overexpressed A549 cells and IL‐25 silenced A549/CDDP cells. B, The effect of exogenous IL‐25 on the activity of NF‐κB signaling pathway in A549 cells. C, QNZ blocks the effect of exogenous IL‐25 on the expression of major vault protein (MVP) and the activity of NF‐κB signaling pathway in A549 cells. *P < 0.01 vs indicated controls, all figures represent the average of three sets of independent experiments