Rabbit Recombinant Monoclonal MVP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--western-blot-img6060.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184)")
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunocytochemistry-immunofluorescence-img66127.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18297.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184)")
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--flow-cytometry-intracellular-img113096.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63935.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (AB240184)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Associated Products
Select an associated product type
3 products for Alternative Product
8 products for Alternative Version
Target data
Function
Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Alternative names
LRP, MVP, Major vault protein, Lung resistance-related protein
Recommended products
Rabbit Recombinant Monoclonal MVP antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13227(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240184 is the carrier-free version of Anti-MVP antibody [EPR13227(B)] ab175239.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--western-blot-img6060.jpg "Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Western blot - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MVP antibody [EPR13227(B)] ab175239).
Lanes 1-4: Merged signal (red and green). Green - Anti-MVP antibody [EPR13227(B)] ab175239 observed at 110 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-MVP antibody [EPR13227(B)] ab175239 Anti-MVP antibody [EPR13227(B)] was shown to specifically react with MVP in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human MVP knockout HeLa cell line ab264817 (knockout cell lysate Human MVP knockout HeLa cell lysate ab257544) was used. Wild-type and MVP knockout samples were subjected to SDS-PAGE. Anti-MVP antibody [EPR13227(B)] ab175239 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-MVP antibody [EPR13227(B)] (Anti-MVP antibody [EPR13227(B)] ab175239) at 1/2000 dilution
Lane 1: Wild-type HeLa lysate at 20 µg
Lane 2: MVP knockout HeLa lysate at 20 µg
Lane 2: Western blot - Human MVP knockout HeLa cell line (Human MVP knockout HeLa cell line ab264817)
Lane 3: A549 lysate at 20 µg
Lane 4: MOLT-4 lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 110 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunocytochemistry-immunofluorescence-img66127.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MVP with purified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/150) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18297.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling MVP with purified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/350. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--flow-cytometry-intracellular-img113096.jpg "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Intracellular Flow Cytometry analysis of A549 cells labelling MVP with purified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/180 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img63935.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling MVP with unpurified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119163.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labeling MVP with unpurified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunocytochemistry-immunofluorescence-img1644.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Immunocytochemistry/Immunofluorescence analysis of A549 cells labeling MVP with unpurified Anti-MVP antibody [EPR13227(B)] ab175239 at a dilution of 1/250 (red) and DAPI staining (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--flow-cytometry-intracellular-img26868.jpg "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)
Intracellular flow cytometrical analysis of permeabilized A549 cells labeling MVP with unpurified Anti-MVP antibody [EPR13227(B)] ab175239 antibody at a dilution of 1/100 (red) compared to a negative control (Rabbit IgG, green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR13227(B)] ab175239).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119163.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--immunocytochemistry-immunofluorescence-img1644.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr13227b-bsa-and-azide-free-ab240184--flow-cytometry-intracellular-img26868.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR13227(B)] - BSA and Azide free (ab240184)")
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