Rabbit Recombinant Monoclonal MVP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--western-blot-img16969.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124264.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097)")
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunocytochemistry-immunofluorescence-img19828.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097)")
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--flow-cytometry-intracellular-img50700.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097)")
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunocytochemistry-immunofluorescence-img94907.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (AB273097)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Tested | Tested | Expected | Not recommended |
| Rat | Not recommended | Tested | Expected | Expected | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates IFNG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
Alternative names
LRP, MVP, Major vault protein, Lung resistance-related protein
Recommended products
Rabbit Recombinant Monoclonal MVP antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23594-106
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273097 is the carrier-free version of Anti-MVP antibody [EPR23594-106] ab273093.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--western-blot-img16969.jpg "Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Western blot - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
This data was developed using the same antibody clone in a different buffer formulation (Anti-MVP antibody [EPR23594-106] ab273093).
Lanes 1-3: Merged signal (red and green). Green - Anti-MVP antibody [EPR23594-106] ab273093 observed at 110 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
Anti-MVP antibody [EPR23594-106] ab273093 Anti-MVP antibody [EPR23594-106] was shown to specifically react with MVP in wild-type HeLa cells in Western blot. Significant decrease (8.7 % of intensity compared to the WT band) of signal was observed when MVP knockout cell line Human MVP knockout HeLa cell line ab264817 (knockout cell lysate Human MVP knockout HeLa cell lysate ab257544) was used.
Anti-MVP antibody [EPR23594-106] ab273093 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-MVP antibody [EPR23594-106] (Anti-MVP antibody [EPR23594-106] ab273093) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lane 2: MVP knockout HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lane 2: Western blot - Human MVP knockout HeLa cell line (Human MVP knockout HeLa cell line ab264817) with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
Lane 3: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 110 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124264.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue labeling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human ovary cancer (PMID: 23739867). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunocytochemistry-immunofluorescence-img19828.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 cells labelling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing cytoplasmic staining in A549 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--flow-cytometry-intracellular-img50700.jpg "Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Flow Cytometry (Intracellular) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/50 dilution (1μg) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunocytochemistry-immunofluorescence-img94907.jpg "Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Immunocytochemistry/ Immunofluorescence - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 μg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8278.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human lung cancer (PMID: 22117969). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102698.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MVP with Anti-MVP antibody [EPR23594-106] ab273093 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in endothelium of human cerebrum (PMID: 14636345). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-MVP antibody [EPR23594-106] ab273093).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img8278.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097), expandable thumbnail](https://content.abcam.com/products/images/mvp-antibody-epr23594-106-bsa-and-azide-free-ab273097--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102698.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MVP antibody [EPR23594-106] - BSA and Azide free (ab273097)")
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